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Single Enzyme Kinetics: A Study of the Yeast Enzyme Candida Antarctica Lipase B

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Single Molecules and Nanotechnology

Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 12))

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Enzymes are a class of (bio)molecules that function as catalysts of (bio)chemical reactions. They highly accelerate the rate of (bio)chemical reactions (Fersht 1985). During the past decades, our knowledge about enzymes has greatly increased. Enzymes are almost always proteins which are very complex structures having many degrees of motional freedom. Much insight into the structures of these and other biomolecules is obtained by X-ray crystallography (Fersht 1985), nuclear magnetic resonance (NMR; Matthews and Van Holde (1996)), and molecular dynamics simulations (MDS) studies (Jensen et al. 2002). Most of the information available on enzymes has been primarily obtained by means of ensemble measurements. Unfortunately, these data do not give further insight in the static and dynamic heterogeneities. This is important as enzymes are not static molecules. The primary structure can fold into many slightly different conformations with different chemical activity (Frauenfelder et al. 1991). A well-studied process, for example, is the binding of carbon monoxide (CO) to myoglobin (Mb).

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Authors and Affiliations

  1. Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium

    Wendy Verheijen, Davey Loos, Frans C. De Schryver & Johan Hofkens

  2. Unité Chimie des Matériaux Inorganiques et Organiques, Université Catholique de Louvain, Bâtiment Lavoisier Place L. Pasteur 1, 1348, Louvain-la-Neuve, Belgium

    Johan Hofkens

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  1. Wendy Verheijen
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  2. Davey Loos
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  3. Frans C. De Schryver
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  4. Johan Hofkens
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Editors and Affiliations

  1. Laboratory of Medical Biophysics, MBB, Karolinska Institute, S-171 77, Stockolm, Sweden

    R. Rigler

  2. Laboratory of Physical Chemistry of Polymers and Membranes Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology (EPFL), CH-1015, Lausanne, Switzerland

    H. Vogel

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Verheijen, W., Loos, D., De Schryver, F.C., Hofkens, J. (2008). Single Enzyme Kinetics: A Study of the Yeast Enzyme Candida Antarctica Lipase B. In: Rigler, R., Vogel, H. (eds) Single Molecules and Nanotechnology. Springer Series in Biophysics, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-73924-1_7

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