Abstract
In this study we demonstrated the presence of a kinase-independent function for cyclin E. Specifically, weobserved that a kinase-deficient cyclin E1mutant can reconstitute cyclin Eās function in cyclin E-null cells. Kinase-deficient cyclin E1 is loaded onto chromatin during G0 ā S progression, it restores MCM incorporation and it facilitates S phase entry of cyclin E-null cells. We also observed that, in wild-type cells, cyclin E is loaded onto DNA during the G0 ā S transition, and it co-localizes with MCM on chromatin. We demonstrated a physical interaction between cyclin E and MCM. We propose that the DNA-bound fraction of cyclin E facilitates MCM loading in a kinase-independent fashion. Our work indicates that, in addition to their well-established function as activators of cyclin-dependent kinases, E-cyclins play a kinase-independent function in cell cycle progression.
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Geng, Y. et al. (2008). A Novel Function for Cyclin E in Cell Cycle Progression. In: Melmed, S., Rochefort, H., Chanson, P., Christen, Y. (eds) Hormonal Control of Cell Cycle. Research and Perspectives in Endocrine Interactions. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-73855-8_4
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DOI: https://doi.org/10.1007/978-3-540-73855-8_4
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