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Inhibition of Cardiomyocyte Automaticity by Electrotonic Application of Inward Rectifier Current from Kir2.1 Expressing Cells

  • Chapter
Biopacemaking

Part of the book series: Series in Biomedical Engineering ((BIOMENG))

Abstract

A biological pacemaker might be created by generation of a cellular construct consisting of cardiac cells that display spontaneous membrane depolarization, and that are electrotonically coupled to surrounding myocardial cells by means of gap junctions. Depending on the frequency of the spontaneously beating cells, frequency regulation might be required. We hypothesized that application of Kir2.1 expressing non-cardiac cells, which provide I K1 to spontaneously active neonatal cardiomyocytes (NCMs) by electrotonic coupling in such a cellular construct, would generate an opportunity for pacemaker frequency control. Non-cardiac Kir2.1 expressing cells were co-cultured with spontaneously active rat NCMs. Electrotonic coupling between the two cell types resulted in hyperpolarization of the cardiomyocyte membrane potential and silencing of spontaneous activity. Either blocking of gap-junctional communication by halothane or inhibition of I K1 by BaCl2 restored the original membrane potential and spontaneous activity of the NCMs. Our results demonstrate the power of electrotonic coupling for the application of specific ion currents into an engineered cellular construct such as a biological pacemaker.

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Authors and Affiliations

  1. Department of Medical Physiology, Heart Lung Center Utrecht, University Medical Center Utrecht, Yalelaan 50, 3584, Utrecht, The Netherlands

    Teun P. de Boer, Toon A. B. van Veen, Marien J. C. Houtman, John A. Jansen, Marc A. Vos & Marcel A. G. van der Heyden

  2. Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands

    Shirley C. M. van Amersfoorth

  3. Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands

    Pieter A. Doevendans

  4. Interuniversity Cardiology Institute of the Netherlands, Utrecht, The Netherlands

    Teun P. de Boer, Shirley C. M. van Amersfoorth & Marcel A. G. van der Heyden

Authors
  1. Teun P. de Boer
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  2. Toon A. B. van Veen
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  3. Marien J. C. Houtman
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  4. John A. Jansen
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  5. Shirley C. M. van Amersfoorth
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  6. Pieter A. Doevendans
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  7. Marc A. Vos
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  8. Marcel A. G. van der Heyden
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Editor information

Editors and Affiliations

  1. Academic Medical Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands

    J. A. E Spaan

  2. Department of Experimental Cardiology, Academic Medical Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands

    Ruben Coronel

  3. Department of Experimental Cardiology Center for Heart Failure Research, Academic Medical Center, Meibergdreef 9, 1105 AZ, Amsterdam

    Jacques M. T. de Bakker

  4. The Heart Lung Center, University Medical Center, Utrecht

    Jacques M. T. de Bakker

  5. The Interuniversity Cardiology, Institute of the Netherlands, Utrecht, The Netherlands

    Jacques M. T. de Bakker

  6. Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy

    Antonio Zaza

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© 2007 Springer-Verlag Berlin Heidelberg

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de Boer, T.P. et al. (2007). Inhibition of Cardiomyocyte Automaticity by Electrotonic Application of Inward Rectifier Current from Kir2.1 Expressing Cells. In: Spaan, J.A.E., Coronel, R., de Bakker, J.M.T., Zaza, A. (eds) Biopacemaking. Series in Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-72110-9_7

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  • DOI: https://doi.org/10.1007/978-3-540-72110-9_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-72109-3

  • Online ISBN: 978-3-540-72110-9

  • eBook Packages: EngineeringEngineering (R0)

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