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Measurement of Retinal Vessel Diameters

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References

  1. Bengtsson B, Krakau CE (1992) Correction of optic disc measurements on fundus photographs. Graefes Arch Clin Exp Ophthalmol 230:24–28

    Article  PubMed  CAS  Google Scholar 

  2. Blum M, Bachmann K, Wintzer D, Riemer T, Vilser W, Strobel J (1999) Noninvasive measurement of the Bayliss effect in retinal autoregulation. Graefes Arch Clin Exp Ophthalmol 237:296–300

    Article  PubMed  CAS  Google Scholar 

  3. Blum M, Brandel C, Muller UA (2005) Myogenic response reduction by high blood glucose levels in human retinal arterioles. Eur J Ophthalmol 15:56–61

    PubMed  CAS  Google Scholar 

  4. Bracher D, Dozzi M, Lotmar W (1979) Measurement of vessel width on fundus photographs. Albrecht Von Graefes Arch Klin Exp Ophthalmol 211:35–48

    PubMed  CAS  Google Scholar 

  5. Brinchmann-Hansen O, Engvold O (1986) Microphotometry of the blood column and the light streak on retinal vessels in fundus photographs. Acta Ophthalmol Scand 179(suppl):9–19

    Google Scholar 

  6. Chen H, Patel V, Wiek J, Rassam S, Kohner E (1994) Vessel diameter changes during the cardiac cycle. Eye (Lond) 8:97–103

    Article  Google Scholar 

  7. Delori FC, Fitch KA, Feke GT, Deupree DM, Weiter JJ (1988) Evaluation of micrometric and microdensitometric methods for measuring the width of retinal vessel images on fundus photographs. Graefes Arch Clin Exp Ophthalmol 226:393–399

    Article  PubMed  CAS  Google Scholar 

  8. Dorner GT, Polska E, Garhofer G, Zawinka C, Frank B, Schmetterer L (2002) Calculation of the diameter of the central retinal artery from noninvasive measurements in humans. Curr Eye Res 25:341–345

    Article  PubMed  Google Scholar 

  9. Dumskyj M, Aldington S, Doré C, Kohner E (1996) The accurate assessment of changes in retinal vessel diameter using multiple frame electrocardiograph synchronised fundus photography. Curr Eye Res 15:625–632

    Article  PubMed  CAS  Google Scholar 

  10. Feke GT, Goger DG, Tagawa H, Delori FC (1987) Laser Doppler technique for absolute measurement of blood speed in retinal vessels. IEEE Trans Biomed Eng 34:673–680

    Article  PubMed  CAS  Google Scholar 

  11. Feke GT, Tagawa H, Deupree DM, Goger DG, Sebag J, Weiter JJ (1989) Blood flow in the normal human retina. Invest Ophthalmol Vis Sci 30:58–65

    PubMed  CAS  Google Scholar 

  12. Formaz F, Riva C, Geiser M (1997) Diffuse luminance flicker increases retinal vessel diameter in humans. Curr Eye Res 16:1252–1257

    Article  PubMed  CAS  Google Scholar 

  13. Frederiksen CA, Jeppesen P, Knudsen ST, Poulsen PL, Mogensen CE, Bek T (2006) The blood pressure-induced diameter response of retinal arterioles decreases with increasing diabetic maculopathy. Graefes Arch Clin Exp Ophthalmol 244:1255–1261

    Article  PubMed  Google Scholar 

  14. Garhofer G, Resch H, Weigert G, Lung S, Simader C, Schmetterer L (2005) Short-term increase of intraocular pressure does not alter the response of retinal and optic nerve head blood flow to flicker stimulation. Invest Ophthalmol Vis Sci 46:1721–1725

    Article  PubMed  Google Scholar 

  15. Garhofer G, Zawinka C, Huemer KH, Schmetterer L, Dorner GT (2003) Flicker light induced vasodilatation in the human retina – Effect of lactate and changes in mean arterial pressure. Invest Ophthalmol Vis Sci 44:5309–5314

    Article  PubMed  Google Scholar 

  16. Garhofer G, Zawinka C, Resch H, Huemer KH, Schmetterer L, Dorner GT (2004) Response of ­retinal vessel diameters to flicker stimulation in patients with early open angle glaucoma. J Glaucoma 13:340–344

    Article  PubMed  CAS  Google Scholar 

  17. Garhofer G, Zawinka C, Resch H, Kothy P, Schmetterer L, Dorner GT (2004) Reduced response of retinal vessel diameters to flicker stimulation in patients with diabetes. Br J Ophthalmol 88:887–891

    Article  PubMed  CAS  Google Scholar 

  18. Grunwald JE, Riva CE, Brucker AJ, Sinclair SH, Petrig BL (1984) Altered retinal vascular response to 100% oxygen breathing in diabetes mellitus. Ophthalmology 91:1447–1452

    PubMed  CAS  Google Scholar 

  19. Grunwald JE, Riva CE, Sinclair SH, Brucker AJ, Petrig BL (1986) Laser Doppler velocimetry study of retinal circulation in diabetes mellitus. Arch Ophthalmol 104:991–996

    Article  PubMed  CAS  Google Scholar 

  20. Guan K, Hudson C, Flanagan JG (2003) Variability and repeatability of retinal blood flow measurements using the Canon Laser Blood Flowmeter. Microvasc Res 65:145–151

    Article  PubMed  Google Scholar 

  21. Gugleta K, Zawinka C, Rickenbacher I, Kochkorov A, Katamay R, Flammer J, Orgul S (2006) Analysis of retinal vasodilation after flicker light stimulation in relation to vasospastic propensity. Invest Ophthalmol Vis Sci 47:4034–4041

    Article  PubMed  Google Scholar 

  22. Hodge JV, Parr JC, Spears GF (1969) Comparison of methods of measuring vessel widths on retinal ­photographs and the effect of fluorescein injection on apparent retinal vessel calibers. Am J Ophthalmol 68:1060–1068

    PubMed  CAS  Google Scholar 

  23. Hogan MJ, Feeney L (1963) The ultrastructure of the retinal blood vessels. I. The large vessels. J Ultrastruct Res 39:10–28

    Article  PubMed  CAS  Google Scholar 

  24. Hubbard LD, Brothers RJ, King WN, Clegg LX, Klein R, Cooper LS, Sharrett AR, Davis MD, Cai J (1999) Methods for evaluation of retinal microvascular abnormalities associated with hypertension/sclerosis in the Atherosclerosis Risk in Communities Study. Ophthalmology 106:2269–2280

    Article  PubMed  CAS  Google Scholar 

  25. Ikram MK, de Jong FJ, Vingerling JR, Witteman JC, Hofman A, Breteler MM, de Jong PT (2004) Are retinal arteriolar or venular diameters associated with markers for cardiovascular disorders? The Rotterdam study. Invest Ophthalmol Vis Sci 45:2129–2134

    Article  PubMed  Google Scholar 

  26. Ikram MK, de Voogd S, Wolfs RC, Hofman A, Breteler MM, Hubbard LD, de Jong PT (2005) Retinal vessel diameters and incident open-angle glaucoma and optic disc changes: the Rotterdam study. Invest Ophthalmol Vis Sci 46:1182–1187

    Article  PubMed  Google Scholar 

  27. Jonas JB, Nguyen XN, Naumann GO (1989) Parapapillary retinal vessel diameter in normal and glaucoma eyes. I. Morphometric data. Invest Ophthalmol Vis Sci 30:1599–1603

    PubMed  CAS  Google Scholar 

  28. Klein R, Klein BE, Knudtson MD, Wong TY, Tsai MY (2006) Are inflammatory factors related to retinal vessel caliber? The Beaver Dam Eye Study. Arch Ophthalmol 124:87–94

    Article  PubMed  Google Scholar 

  29. Klein R, Sharrett AR, Klein BE, Chambless LE, Cooper LS, Hubbard LD, Evans G (2000) Are retinal arteriolar abnormalities related to atherosclerosis?: The Atherosclerosis Risk in Communities Study. Arterioscler Thromb Vasc Biol 20:1644–1650

    Article  PubMed  CAS  Google Scholar 

  30. Knudtson MD, Klein BE, Klein R, Wong TY, Hubbard LD, Lee KE, Meuer SM, Bulla CP (2004) Variation associated with measurement of retinal vessel diameters at different points in the pulse cycle. Br J Ophthalmol 88:57–61

    Article  PubMed  CAS  Google Scholar 

  31. Knudtson MD, Lee KE, Hubbard LD, Wong TY, Klein R, Klein BE (2003) Revised formulas for summarizing retinal vessel diameters. Curr Eye Res 27:143–149

    Article  PubMed  Google Scholar 

  32. Kolodjaschna J, Berisha F, Lung S, Schaller G, Polska E, Jilma B, Wolzt M, Schmetterer L (2004) LPS-induced microvascular leukocytosis can be assessed by blue-field entoptic phenomenon. Am J Physiol Heart Circ Physiol 287:H691–H694

    Article  PubMed  CAS  Google Scholar 

  33. Leung H, Wang JJ, Rochtchina E, Wong TY, Klein R, Mitchell P (2004) Impact of current and past blood pressure on retinal arteriolar diameter in an older population. J Hypertens 22:1543–1549

    Article  PubMed  CAS  Google Scholar 

  34. Li H, Hsu W, Lee ML & Wong TY (2005) Automatic grading of retinal vessel caliber. IEEE Trans Biomed Eng 52:1352–1355.

    Google Scholar 

  35. Liew G, Sharrett AR, Kronmal R, Klein R, Wong TY, Mitchell P, Kifley A, Wang JJ (2007) Measurement of retinal vascular caliber: issues and alternatives to using the arteriole to venule ratio. Invest Ophthalmol Vis Sci 48:52–57

    Article  PubMed  Google Scholar 

  36. Littmann H (1982) Zur Bestimmung der wahren Größe eines Objektes auf dem Hintergrund des lebenden Auges. Klin Monatsbl Augenheilkd 180:286–289

    Article  PubMed  CAS  Google Scholar 

  37. Lobeck E (1935) Über Messungen am Augenhintergrund. Graefes Arch Clin Exp Ophthalmol 133:152–156

    Article  Google Scholar 

  38. Lobeck E (1938) Die Breite der Netzhautgefäße als Differentialdiagnosticum bei Hochdruck- und Nierenkrankheiten. Klin Monatsbl Augenheilkd 1938:765

    Google Scholar 

  39. Lowell J, Hunter A, Steel D, Basu A, Ryder R, Kennedy RL (2004) Measurement of retinal vessel widths from fundus images based on 2-D modeling. IEEE Trans Med Imaging 23:1196–1204

    Article  PubMed  Google Scholar 

  40. Luksch A, Garhofer G, Imhof A, Polak K, Polska E, Dorner GT, Anzenhofer S, Wolzt M, Schmetterer L (2002) Effect of inhalation of different mixtures of O(2) and CO(2) on retinal blood flow. Br J Ophthalmol 86:1143–1147

    Article  PubMed  CAS  Google Scholar 

  41. Mandecka A, Dawczynski J, Blum M, Muller N, Kloos C, Wolf G, Vilser W, Hoyer H, Muller UA (2007) Influence of flickering light on the retinal vessels in diabetic patients. Diabetes Care 30:3048–3052

    Article  PubMed  Google Scholar 

  42. Mikuni M (1959) Eine Methode zur Messung der Netzhautgefäßweite. Klin Monatsbl Augenheilkd 135:205–211

    CAS  Google Scholar 

  43. Mitchell P, Leung H, Wang JJ, Rochtchina E, Lee AJ, Wong TY, Klein R (2005) Retinal vessel diameter and open-angle glaucoma: the Blue Mountains Eye Study. Ophthalmology 112:245–250

    Article  PubMed  Google Scholar 

  44. Nagel E, Vilser W, Lanzl I (2004) Age, blood pressure, and vessel diameter as factors influencing the arterial retinal flicker response. Invest Ophthalmol Vis Sci 45:1486–1492

    Article  PubMed  Google Scholar 

  45. Nagel E, Vilser W, Lanzl IM, Lanzi IM (2001) Retinal vessel reaction to short-term IOP elevation in ocular hypertensive and glaucoma patients. Eur J Ophthalmol 11:338–344

    PubMed  CAS  Google Scholar 

  46. Parr JC, Spears GF (1974) General caliber of the retinal arteries expressed as the equivalent width of the central retinal artery. Am J Ophthalmol 77:472–477

    PubMed  CAS  Google Scholar 

  47. Parr JC, Spears GF (1974) Mathematic relationships between the width of a retinal artery and the widths of its branches. Am J Ophthalmol 77:478–483

    PubMed  CAS  Google Scholar 

  48. Polak K, Dorner GT, Kiss B, Polska E, Findl O, Rainer G, Eichler HG, Schmetterer L (2000) Evaluation of the Zeiss retinal vessel analyser. Br J Ophthalmol 84:1285–1290

    PubMed  Google Scholar 

  49. Polak K, Schmetterer L, Riva CE (2002) Influence of flicker frequency on flicker induced changes of retinal vessel diameters. Invest Ophthalmol Vis Sci 43:2721–2726

    PubMed  Google Scholar 

  50. Quigley M, Cohen S (1999) A new pressure attenuation index to evaluate retinal circulation. A link to protective factors in diabetic retinopathy. Arch Ophthalmol 117:84–89

    Article  PubMed  CAS  Google Scholar 

  51. Rader J, Feuer WJ, Anderson DR (1994) Peripapillary vasoconstriction in the glaucomas and the anterior ischemic optic neuropathies. Am J Ophthalmol 117:72–80

    PubMed  CAS  Google Scholar 

  52. Riva CE, Grunwald JE, Sinclair SH (1983) Laser Doppler Velocimetry study of the effect of pure oxygen breathing on retinal blood flow. Invest Ophthalmol Vis Sci 24:47–51

    PubMed  CAS  Google Scholar 

  53. Riva CE, Logean E, Falsini B (2005) Visually evoked hemodynamical response and assessment of neurovascular coupling in the optic nerve and retina. Prog Retin Eye Res 24:183–215

    Article  PubMed  Google Scholar 

  54. Riva CE, Salgarello T, Logean E, Colotto A, Galan EM, Falsini B (2004) Flicker-evoked response measured at the optic disc rim is reduced in ocular hypertension and early glaucoma. Invest Ophthalmol Vis Sci 45:3662–3668

    Article  PubMed  Google Scholar 

  55. Sandor T, Rhie FH, Soeldner JS, Gleason RE, Rand LI (1981) Reproducibility of the densitometric analysis of fluorescein angiograms. Int J Biomed Comput 12:401–418

    Article  PubMed  CAS  Google Scholar 

  56. Scheie HG (1953) Evaluation of ophthalmoscopic changes of hypertension and arteriolar sclerosis. AMA Arch Ophthalmol 49:117–138

    Article  PubMed  CAS  Google Scholar 

  57. Sharrett AR, Hubbard LD, Cooper LS, Sorlie PD, Brothers RJ, Nieto FJ, Pinsky JL, Klein R (1999) Retinal arteriolar diameters and elevated blood pressure: the Atherosclerosis Risk in Communities Study. Am J Epidemiol 150:263–270

    Article  PubMed  CAS  Google Scholar 

  58. Vilser W (1987) New diagnostic possibilities with a retinal measuring system. Jena Rev 32:76–78

    Google Scholar 

  59. Vilser W, Königsdörffer E, Brandt H (1979) Längenmessungen mittels Planplattenmikrometrie am menschlichen Augenhintergrund. Graefes Arch Clin Exp Ophthalmol 212:109–115

    Article  CAS  Google Scholar 

  60. Vilser W, Tirsch P, Münch K, Kleen W, Klein S (1990) Automatische Gefäßweitenmessung. Folia Ophthalmol 15:297–303

    Google Scholar 

  61. Vilser W, Münch K, Saleh K, Kassner Ch, Seifert BU, Henning G (2004) Vessel model for the validation of retinal vessel analysis. Biomed Tech, 49: (Erg.-Bd.2,T.2)–816

    Google Scholar 

  62. Wang JJ, Liew G, Klein R, Rochtchina E, Knudtson MD, Klein BE, Wong TY, Burlutsky G, Mitchell P (2007) Retinal vessel diameter and cardiovascular mortality: pooled data analysis from two older populations. Eur Heart J 28:1984–1992

    Article  PubMed  CAS  Google Scholar 

  63. Wang L, Wong TY, Sharrett AR, Klein R, Folsom AR, Jerosch-Herold M (2008) Relationship between retinal arteriolar narrowing and myocardial perfusion: multi-ethnic study of atherosclerosis. Hypertension 51:119–126

    Article  PubMed  CAS  Google Scholar 

  64. Wong TY, Hubbard LD, Klein R, Marino EK, Kronmal R, Sharrett AR, Siscovick DS, Burke G, Tielsch JM (2002) Retinal microvascular abnormalities and blood pressure in older people: the Cardiovascular Health Study. Br J Ophthalmol 86:1007–1013

    Article  PubMed  CAS  Google Scholar 

  65. Wong TY, Klein R, Couper DJ, Cooper LS, Shahar E, Hubbard LD, Wofford MR, Sharrett AR (2001) Retinal microvascular abnormalities and incident stroke: the Atherosclerosis Risk in Communities Study. Lancet 358:1134–1140

    Article  PubMed  CAS  Google Scholar 

  66. Wong TY, Klein R, Sharrett AR, Duncan BB, Couper DJ, Tielsch JM, Klein BE, Hubbard LD (2002) Retinal arteriolar narrowing and risk of coronary heart disease in men and women. The Atherosclerosis Risk in Communities Study. JAMA 287:1153–1159

    Article  PubMed  Google Scholar 

  67. Wong TY, Knudtson MD, Klein R, Klein BE, Meuer SM, Hubbard LD (2004) Computer-assisted measurement of retinal vessel diameters in the Beaver Dam Eye Study: methodology, correlation between eyes, and effect of refractive errors. Ophthalmology 111:1183–1190

    Article  PubMed  Google Scholar 

  68. Wong TY, Shankar A, Klein R, Klein BE, Hubbard LD (2004) Prospective cohort study of retinal vessel diameters and risk of hypertension. BMJ 329:79

    Article  PubMed  Google Scholar 

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Garhöfer, G., Vilser, W. (2012). Measurement of Retinal Vessel Diameters. In: Schmetterer, L., Kiel, J. (eds) Ocular Blood Flow. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-69469-4_6

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