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International Conference “New Technologies, Development and Applications”

NT 2018: New Technologies, Development and Application pp 480–490Cite as

The Effect of Concentration of Methanol as a Solvent on the Antioxidative Activity of Sage Extract

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Part of the book series: Lecture Notes in Networks and Systems ((LNNS,volume 42))

Abstract

The production of high quality plant extracts is the basis for the production of herbal preparations and the isolation of bioactive ingredients. Recently, there is a tendency of replacing synthetic antioxidants, due to their toxic and potentially cancerous effects, with natural antioxidants. For this reason, it is necessary to intensify research of antioxidative and biological effects of plant extracts, as well as the influence of the process conditions of extraction on the mentioned effects and the yield of extraction. In this paper, the antioxidative activity of sage extract was tested, depending on the concentration of methanol as a solvent in the process of maceration with occasional mixing. Total phenolic and flavonoid content in obtained sage extracts was determined spectrophotometrically. The radical scavenging capacity was determined by the DPPH method, where the extract concentration required for the neutralization of 50% of the initial concentration of the DPPH radical was also determined.

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References

  1. Beattie, S.D.: Bioenergetics and oxidative metabolism. In: Devlin, T.M. (ed.) Textbook of Biochemistry with Clinical Correlations, 6th edn, pp. 978–989. Wiley-Liss, New York (2006)

    Google Scholar 

  2. Kancheva, V.D.: Phenolic antioxidants – radical-scavenging and chainbreaking activity: a comparative study. Eur. J. Lipid Sci. Technol. 111, 1072–1089 (2009)

    Article  Google Scholar 

  3. Shi, H., Noguchi, N., Niki, E.: Introducing natural antioxidants. In: Pokorny, J., Yanishlieva, N., Gordon, M. (eds.) Antioxidants in Food, Practical Applications, 1st edn, pp. 22–70. Woodhead Publishing Limited, Cambridge (2001)

    Google Scholar 

  4. Reische, D.W., Lillard, D.A., Eitenmiller, R.R.: Antioxidants. In: Akoh, C.C., Min, D.B. (eds.) Food Lipids: Chemistry, Nutrition and Biotechnology, 3rd edn, pp. 410–413. CRC Press, Boca Raton (2008)

    Google Scholar 

  5. Halliwell, B., Murcia, M.A., Chrico, S., Aruoma, O.I.: Free radicals and antioxidants in food and in vivo: what they do and how they work. Food Sci. Nutr. 35, 7–20 (1995)

    Google Scholar 

  6. Lobo, V., Patil, A., Phatak, A., Chandra, N.: Free radicals, antioxidants and functional foods: impact on human health. Pharm. Rev. 4(8), 118–126 (2010)

    Article  Google Scholar 

  7. Pokorný, J., Yanishlieva, N.V., Gordon, H.: Antioxidants in Food – Practical Applications, pp. 22–70. Woodhead Publishing, Cambridge (2001)

    Google Scholar 

  8. Dai, J., Mumper, R.: Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15, 7313–7352 (2010)

    Article  Google Scholar 

  9. Cushnie, T.P., Lamb, A.J.: Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents 26, 343–356 (2005)

    Article  Google Scholar 

  10. Katalinić, V., Smole, M.S., Skroza, D., Generalić, I., Abramović, H., Miloš, M., Ljubenkov, I., Piskernik, S., Pezo, I., Terpinc, P., Boban, M.: Polyphenolic profile, antioxidant properties and antimicrobal activity of grape skin extracts of 14 Vitis vinifera varieties grown in Dalmatia (Croatia). Food Chem. 119, 715–723 (2010)

    Article  Google Scholar 

  11. Castellano, G., Tena, J., Torrens, F.: Classification of phenolic compounds by chemical structural indicators and its relation to antioxidant properties of Posidonia Oceanica (L.) Delile. MATCH Commun. Math. Comput. Chem. 67, 231–250 (2012)

    Google Scholar 

  12. Heim, K.E., Tagliafero, A.R., Bobilya, D.J.: Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 13, 572–584 (2002)

    Article  Google Scholar 

  13. Mandal, S.M., Chakraborty, D., Dey, S.: Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signal. Behav. 5(4), 359–368 (2010)

    Article  Google Scholar 

  14. Zhang, Y., Seeram, N.P., Lee, R., Feng, L., Hebe, D.: Isolation and identification of strawberry phenolics with antioxidant and human cancer cell antiproliferative properties. J. Agric. Food Chem. 56, 670–675 (2008)

    Article  Google Scholar 

  15. Harborne, J.B., Baxter, H. (eds.): The Handbook of Natural Flavonoids, vol. 2. John Wiley, Chichester (1999)

    Google Scholar 

  16. Buhler, D., Miranda, C.: Antioxidant action of flavonoids. Micronutrients Information Center, Linus Pauling Institute, London (2000)

    Google Scholar 

  17. Xu, B.J., Chang, S.K.: A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci. 77(2), 159–166 (2007)

    Article  Google Scholar 

  18. Albu, S., Joyce, E., Paniwnyk, L., Lorimer, J.P., Mason, T.J.: Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry. Ultrason. Sonochem. 11, 261–265 (2004)

    Article  Google Scholar 

  19. Bimakr, M., Rahman, R.A., Taip, F.S., Ganjloo, A., Md Salleh, L., Selamat, J., Hamid, A., Zaidul, I.S.M.: Comparision of different extraction methods for the extraction of major flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioprod. Process. 89, 67–72 (2011)

    Article  Google Scholar 

  20. Ross, K.A., Beta, T., Arntfield, S.D.: A comparative study on the phenolic acids identified and quantified in dry beans using HPLC as affected by different extraction and hydrolysis methods. Food Chem. 113, 336–344 (2009)

    Article  Google Scholar 

  21. Altiok, E., Baycin, D., Bayraktar, O., Ülkü, S.: Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin. Sep. Purif. Technol. 62, 342–348 (2008)

    Article  Google Scholar 

  22. Shi, J., Nawaz, H., Pohorly, J., Mittal, G., Kakuda, Y., Jiang, Y.: Extraction of polyphenolics from plant material for functional foods-engineering and technology. Food Rev. Int. 21, 139–166 (2005)

    Article  Google Scholar 

  23. Shan, B., Cai, Y.Z., Sun, M., Corke, H.: Antioxidant capacity of 26 spices extracts and characterization of their phenolic constituents. J. Agric. Food Chem. 53, 7749–7759 (2005)

    Article  Google Scholar 

  24. Guyot, S., Marnet, N., Drilleau, J.: Thiolysis-HPLC characterization of apple procyanidins covering a large range of polymerization states. J. Agric. Food Chem. 49, 14–20 (2001)

    Article  Google Scholar 

  25. Mattila, P., Kumpulainen, J.J.: Determination of free and total phenolic acids in plant derived foods by HPLC and diode array detection. J. Agric. Food Chem. 50(13), 3660–3667 (2002)

    Article  Google Scholar 

  26. Robards, K.: Strategies for the determination of bioactive phenols in plants, fruit and vegetables. J. Chromatogr. A 2003, 657–691 (1000)

    Google Scholar 

  27. Areias, F.M., Valentäo, P., Andrade, P.B., Ferreres, F., Seabra, R.M.: Flavonoids and phenolic acids of sage: influence of some agricultural factors. J. Agric. Food Chem. 48, 6081–6084 (2000)

    Article  Google Scholar 

  28. Durling, N.E., Grey, O.J., Webby, R.F., Mitchell, K.A., Foo, L.Y., Perry, N.B.: Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol-water mixtures. Food Chem. 101, 1417–1424 (2007)

    Article  Google Scholar 

  29. McDonald, S., Prenzler, P.D., Antolovich, M., Robards, K.: Phenolic content and antioxidant activity of olive extract. Food Chem. 73, 73–84 (2001)

    Article  Google Scholar 

  30. Stanković, M.: Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. J. Sci. 33, 63–72 (2011)

    Google Scholar 

  31. Pourmorad, F., Hosseinimehr, S.J., Shahabimajd, N.: Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. J. Biotechnol. 5(11), 1142–1145 (2006)

    Google Scholar 

  32. Pontis, J.A., da Costa, L.A.M.A., da Silva, S.J.R., Flach, A.: Color, phenolic, and flavonoid content and antioxidant activity of honey from Roraima, Brazil. Food Sci. Technol. 34(1), 69–73 (2014)

    Article  Google Scholar 

  33. Brighente, I.M.C., Dias, M., Verdi, L.G., Pizzolatti, M.G.: Antioxidant activity and total phenolic content of some Brazilian species. Pharm. Biol. 45(2), 156–161 (2007)

    Article  Google Scholar 

  34. Naczk, M., Shahidi, F.: Extraction and analysis of phenolics in food. J. Chromatogr. A 1054(1–2), 95–111 (2004)

    Article  Google Scholar 

  35. Chan, S.W., Lee, C.Y., Yap, C.F., Wan Aida, W.M., Ho, C.W.: Optimisation of extraction conditions for phenolic compounds from limau purut (Citrus hystrix) peels. Int. Food Res. J. 16, 203–219 (2009)

    Google Scholar 

  36. Chirinos, R., Rogez, H., Campos, D., Pedreschi, R., Larondelle, Y.: Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz and Pavón) tubers. Sep. Purif. Technol. 55(2), 217–225 (2007)

    Article  Google Scholar 

  37. Bouterfas, K., Mehdadi, Z., Benmansour, D., Khaled, M.B., Bouterfas, M., Latreche, A.: Optimization of extraction conditions of some phenolic compounds from white horehound (Marrubium vulgare L.) Leaves. Int. J. Org. Chem. 4, 292–308 (2014)

    Article  Google Scholar 

  38. Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., Ju, Y.H.: Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J. Food Drug Anal. 22, 296–302 (2014)

    Article  Google Scholar 

  39. Złotek, U., Mikulska, S., Nagajek, M., Świeca, M.: The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi J. Biol. Sci. 23(5), 628–633 (2016)

    Article  Google Scholar 

  40. Kim, I.S., Yang, M.R., Lee, O.H., Kang, S.H.: Antioxidant activities of hot water extracts from various spices. Int. J. Mol. Sci. 12(6), 4120–4131 (2011)

    Article  Google Scholar 

  41. Butsat, S., Siriamornpun, S.: Effect of solvent types and extraction times on phenolic and flavonoid contents and antioxidant activity in leaf extracts of Amomum chinense C. Int. Food Res. J. 23(1), 180–187 (2016)

    Google Scholar 

  42. Teixeira, T.S., Vale, R.C., Almeida, R.R., Ferreira, T.P.S., Guimarães, L.G.L.: Antioxidant potential and its correlation with the contents of phenolic compounds and flavonoids of methanolic extracts from different medicinal plants. Revista Virtual de Química 9(4), 1546–1559 (2017)

    Article  Google Scholar 

  43. Mohammedi, Z., Atik, F.: Impact of solvent extraction type on total polyphenols content and biological activity from Tamarix Aphylla (L.) Karst. Int. J. Pharma Bio Sci. 2(1), 609–615 (2011)

    Google Scholar 

  44. Turkmen, N., Sari, F., Velioglu, Y.S.: Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem. 99, 835–841 (2006)

    Article  Google Scholar 

  45. Zhang, H.Y., Sun, Y.M., Wang, X.L.: Substituent effects on O-H bond dissociation enthalpies and ionization potentials of catechols: a DFT study and its implications in the rational design of phenolic antioxidants and elucidation of structure–activity relationships for flavonoid antioxidants. Chem. Eur. J. 9(2), 502–508 (2003)

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. University of Tuzla, 75000, Tuzla, Bosnia and Herzegovina

    Selma Osmić, Sabina Begić & Vladan Mićić

  2. University of East Sarajevo, 75400, Zvornik, Bosnia and Herzegovina

    Sabina Begić & Vladan Mićić

Authors
  1. Selma Osmić
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  2. Sabina Begić
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  3. Vladan Mićić
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Correspondence to Selma Osmić .

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Editors and Affiliations

  1. Technical Faculty Bihać, University of Bihać, Bihać, Bosnia and Herzegovina

    Isak Karabegović

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Osmić, S., Begić, S., Mićić, V. (2019). The Effect of Concentration of Methanol as a Solvent on the Antioxidative Activity of Sage Extract. In: Karabegović, I. (eds) New Technologies, Development and Application. NT 2018. Lecture Notes in Networks and Systems, vol 42. Springer, Cham. https://doi.org/10.1007/978-3-319-90893-9_56

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