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Current Advances in Fern Research pp 99–120Cite as

Morphogenic Events in Ferns: Single and Multicellular Explants In Vitro

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Abstract

In spite of the recent description of somatic embryogenesis for ferns, there are a lot of evidences that the morphogenic potential of their cells was the objective of numerous studies. The chapter tries to give the summary of the information of morphogenic potential of both fern generations. The fern body provides a source of various explants, both single-celled and multicellular. Under various plant growth regulator regimes, plant regeneration occurs; however, it is stressed that for some of developmental stages, hormone-free medium is required. For numerous species, the system of sporophyte regeneration was presented and discussed. The attention is paid for callus and cell suspension, and their initiation and culture are maintained as the stage of the great potential which might be explored for fern somatic cell manipulation. Protoplasts are the objective of somatic cell genetic manipulation in plants; however, for ferns the number of papers involving protoplast isolation and their subsequent culture is scant. Independently, the attention is paid for green globular bodies, multicellular structures with unlimited shoot regeneration potential.

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Abbreviations

0.5MS medium:

½ Strength MS medium

2,4,5-T:

2,4,5-Trichlorophenoxyacetic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

2-iP:

N6-(2-Isopentenyl adenine)

AC:

Active charcoal

BA:

6-Benzylaminopurine

CH:

Casein hydrolysate

CM:

Coconut milk

CPW solution:

Mineral salt complex (Frearson et al. 1973)

GA3 :

Gibberellic acid

JA:

Jasmonic acid

MFMM:

Murashige Fern Multiplication Medium

MS medium:

Murashige and Skoog medium (1962)

NAA:

α-Naphthaleneacetic acid

SE:

Somatic embryo

SERK :

Somatic embryogenesis receptor kinase gene

TDZ:

Thidiazuron-1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea

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Authors and Affiliations

  1. Department of Experimental Botany, Polish Academy of Sciences, Botanical Garden – Center for Biological Diversity Conservation in Warsaw, Warsaw, Poland

    Jan Jarosław Rybczyński, Karolina Tomiczak, Małgorzata Grzyb & Anna Mikuła

Authors
  1. Jan Jarosław Rybczyński
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  2. Karolina Tomiczak
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  3. Małgorzata Grzyb
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  4. Anna Mikuła
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Correspondence to Jan Jarosław Rybczyński .

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  1. Department of Organisms and Systems Biology, Oviedo University, Oviedo, Spain

    Helena Fernández

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Rybczyński, J.J., Tomiczak, K., Grzyb, M., Mikuła, A. (2018). Morphogenic Events in Ferns: Single and Multicellular Explants In Vitro. In: Fernández, H. (eds) Current Advances in Fern Research. Springer, Cham. https://doi.org/10.1007/978-3-319-75103-0_5

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