Skip to main content

Dentin Basic Structure, Composition, and Function

  • Chapter
  • First Online:
The Root Canal Anatomy in Permanent Dentition

Abstract

Dentin is the largest structural component of the human tooth. Dentin provides support to enamel, preventing enamel fractures during occlusal loading. It also protects the pulp from microbial and other potentially harmful stimuli. As vital tissue, dentin is not only a passive mechanical barrier between the oral environment and the pulp tissue but, in many ways, participates in the overall protection of the continuum of the hard and soft tissue often referred as the dentin-pulp complex. For example, dentin contains several growth factors that may be liberated during wear or caries and participate in the regulation of the defensive reaction at the dentin-pulp border or the pulp proper. Odontoblasts project their cell processes into dentinal tubules, and also therefore the division of the “vital” pulp and “dead” mineralized dentin is artificial. Different parts of the dentin in a particular tooth may also qualitatively differ from each other, which enables it to meet the requirements of that specific location.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 199.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Tjäderhane L, Carrilho MR, Breschi L, Tay FR, Pashley DH. Dentin basic structure and composition – an overview. Endod Topics. 2012;20:3–29.

    Article  Google Scholar 

  2. Mitsiadis TA, Graf D. Cell fate determination during tooth development and regeneration. Birth Defects Res C Embryo Today. 2009;87:199–211.

    Article  Google Scholar 

  3. Tjäderhane L, Koivumäki S, Pääkkönen V, Ilvesaro J, Soini Y, Salo T, Metsikkö K, Tuukkanen J. Polarity of mature human odontoblasts. J Dent Res. 2013;92:1011–6.

    Article  Google Scholar 

  4. Tjäderhane L, Haapasalo M. Dentin-pulp border: dynamic interface between hard and soft tissues. Endod Topics. 2012;20:52–84.

    Article  Google Scholar 

  5. Goldberg M, Septier D, Bourd K, Hall R, Jeanny JC, Jonet L, Colin S, Tager F, Chaussain-Miller C, Garabédian M, George A, Goldberg H, Menashi S. The dentino-enamel junction revisited. Connect Tissue Res. 2002;43:482–9.

    Article  Google Scholar 

  6. Gallagher RR, Demos SG, Balooch M, Marshall GW Jr, Marshall SJ. Optical spectroscopy and imaging of the dentin-enamel junction in human third molars. J Biomed Mater Res A. 2003;64:372–7.

    Article  Google Scholar 

  7. Marshall SJ, Balooch M, Habelitz S, Balooch G, Gallagher R, Marshall GW. The dentino-enamel junction – a natural, multilevel interface. J Eur Ceram Soc. 2003;23:2897–904.

    Article  Google Scholar 

  8. Radlanski RJ, Renz H. Insular dentin formation pattern in human odontogenesis in relation to the scalloped dentino-enamel junction. Ann Anat. 2007;189:243–50.

    Article  Google Scholar 

  9. Arsenault AL, Robinson BW. The dentino-enamel junction: a structural and microanalytical study of early mineralization. Calcif Tissue Int. 1989;45:111–21.

    Article  Google Scholar 

  10. Hayashi Y. High resolution electron microscopy in the dentino-enamel junction. J Electron Microsc. 1992;41:387–91.

    Google Scholar 

  11. Diekwisch TG, Berman BJ, Genters S, Slavkin HC. Initial enamel crystals are not spatially associated with mineralized dentin. Cell Tissue Res. 1998;279:149–67.

    Article  Google Scholar 

  12. Lin CP, Douglas WH, Erlandsen SL. Scanning electron microscopy of type I collagen at the dentin-enamel junction of human teeth. J Histochem Cytochem. 1993;41:381–8.

    Article  Google Scholar 

  13. Ohsaki Y, Nagata K. Type III collagen is a major component of interodontoblastic fibers of the developing mouse molar root. Anat Rec. 1994;240:308–13.

    Article  Google Scholar 

  14. Tjäderhane L, Hietala E-L, Larmas M. Mineral element analyses of carious and intact dentin by electron probe microanalyser combined with back-scattered electron image. J Dent Res. 1995;74:1770–4.

    Article  Google Scholar 

  15. Wang RZ, Weiner S. Strain-structure relations in human teeth using Moiré fringes. J Biomech. 1998;31:135–41.

    Article  Google Scholar 

  16. Tesch W, Eidelman N, Roschger P, Goldenberg F, Klaushofer K, Fratzl P. Graded microstructure and mechanical properties of human crown dentin. Calcif Tissue Int. 2001;69:147–57.

    Article  Google Scholar 

  17. Zaslansky P, Zabler S, Fratzl P. 3D variations in human crown dentin tubule orientation: a phase-contrast microtomography study. Dent Mater. 2010;26:e1–10.

    Article  Google Scholar 

  18. Zaslansky P, Friesem AA, Weiner S. Structure and mechanical properties of the soft zone separating bulk dentin and enamel in crowns of human teeth: insight into tooth function. J Struct Biol. 2006;153:188–99.

    Article  Google Scholar 

  19. Johannessen LB. Dentine apposition in the mandibular first molars of albino rats. Arch Oral Biol. 1961;5:81–91.

    Article  Google Scholar 

  20. Becker J, Schuppan D, Benzian H, Bals T, Hahn EG, Cantaluppi C, Reichart P. Immunohistochemical distribution of collagens types IV, V and VI and of pro-collagen types I and III in human alveolar bone and dentine. J Histochem Cytochem. 1986;34:1417–29.

    Article  Google Scholar 

  21. De Coster PJ. Dentin disorders: anomalies of dentin formation and structure. Endod Topics. 2012;21:41–61.

    Article  Google Scholar 

  22. Karjalainen S, Söderling E, Pelliniemi L, Foidart JM. Immunohistochemical localization of types I and III collagen and fibronectin in the dentine of carious human teeth. Arch Oral Biol. 1986;31:801–6.

    Article  Google Scholar 

  23. Magloire H, Joffre A, Hartman DJ. Localization and synthesis of type III collagen and fibronectin in human reparative dentine. Immunoperoxidase and immunogold staining. Histochemistry. 1988;88:141–9.

    Article  Google Scholar 

  24. Mjör IA, Nordahl I. The density and branching of dentinal tubules in human teeth. Arch Oral Biol. 1996;41:401–12.

    Article  Google Scholar 

  25. Nalbandian J, Gonzales F, Sognnaes RF. Sclerotic age changes in root dentin of human teeth as observed by optical, electron, and X-ray microscopy. J Dent Res. 1960;39:598–607.

    Article  Google Scholar 

  26. Gotliv BA, Veis A. Peritubular dentin, a vertebrate apatitic mineralized tissue without collagen: role of a phospholipid-proteolipid complex. Calcif Tissue Int. 2007;81:191–205.

    Article  Google Scholar 

  27. Smith AJ, Scheven BA, Takahashi Y, Ferracane JL, Shelton RM, Cooper PR. Dentine as a bioactive extracellular matrix. Arch Oral Biol. 2012;57:109–21.

    Article  Google Scholar 

  28. Carrigan PJ, Morse DR, Furst ML, Sinai IH. A scanning electron microscopic evaluation of human dentinal tubules according to age and location. J Endod. 1984;10:359–63.

    Article  Google Scholar 

  29. Schellenberg U, Krey G, Bosshardt D, Nair PN. Numerical density of dentinal tubules at the pulpal wall of human permanent premolars and third molars. J Endod. 1992;18:104–9.

    Article  Google Scholar 

  30. Mjör IA, Smith MR, Ferrari M, Mannocci F. The structure of dentine in the apical region of human teeth. Int Endod J. 2001;34:346–53.

    Article  Google Scholar 

  31. Harrán Ponce E, Canalda Sahli C, Vilar Fernandez JA. Study of dentinal tubule architecture of permanent upper premolars: evaluation by SEM. Aust Endod J. 2001;27:66–72.

    Article  Google Scholar 

  32. Vasiliadis L, Darling AI, Levers BG. The amount and distribution of sclerotic human root dentine. Arch Oral Biol. 1983;28:645–9.

    Article  Google Scholar 

  33. Paqué F, Luder HU, Sener B, Zehnder M. Tubular sclerosis rather than the smear layer impedes dye penetration into the dentine of endodontically instrumented root canals. Int Endod J. 2006;39:18–25.

    Article  Google Scholar 

  34. Thaler A, Ebert J, Petschelt A, Pelka M. Influence of tooth age and root section on root dentine dye penetration. Int Endod J. 2008;41:1115–22.

    Article  Google Scholar 

  35. Montoya C, Arango-Santander S, Peláez-Vargas A, Arola D, Ossa EA. Effect of aging on the microstructure, hardness and chemical composition of dentin. Arch Oral Biol. 2015;60:1811–20.

    Article  Google Scholar 

  36. Arola D, Ivancik J, Majd H, Fouad A, Bajaj D, Zhang X-Y, Eidelman N. Microstructure and mechanical behavior of radicular and coronal dentin. Endod Topics. 2012;20:30–51.

    Article  Google Scholar 

  37. Bajaj D, Sundaram N, Nazari A, Arola D. Age, dehydration and fatigue crack growth in dentin. Biomaterials. 2006;27:2507–17.

    Article  Google Scholar 

  38. Arola D, Reprogel RK. Effects of aging on the mechanical behavior of human dentin. Biomaterials. 2005;26:4051–61.

    Article  Google Scholar 

  39. Martin-De Las Heras S, Valenzuela A, Overall CM. The matrix metalloproteinase gelatinase A in human dentine. Arch Oral Biol. 2000;45:757–65.

    Article  Google Scholar 

  40. Tersariol IL, Geraldeli S, Minciotti CL, Nascimento FD, Pääkkönen V, Martins MT, Carrilho MR, Pashley DH, Tay FR, Salo T, Tjäderhane L. Cysteine cathepsins in human dentin-pulp complex. J Endod. 2010;36:475–81.

    Article  Google Scholar 

  41. Nascimento FD, Minciotti CL, Geraldeli S, Carrilho MR, Pashley DH, Tay FR, Nader HB, Salo T, Tjäderhane L, Tersariol ILS. Cysteine cathepsins in human carious dentin. J Dent Res. 2011;90:506–11.

    Article  Google Scholar 

  42. Tjäderhane L. Dentin bonding: can we make it last? Oper Dent. 2015;40:4–18.

    Article  Google Scholar 

  43. Tjäderhane L, Buzalaf MAR, Carrilho M, Chaussain C. Matrix metalloproteinases and other matrix proteinases in relation to cariology: the era of “Dentin Degradomics”. Caries Res. 2015;49:193–208.

    Article  Google Scholar 

  44. Do D, Orrego S, Majd H, Ryou H, Mutluay MM, Xu HH, Arola DD. Accelerated fatigue of dentin with exposure to lactic acid. Biomaterials. 2013;34:8650–9.

    Article  Google Scholar 

  45. Orrego S, Xu H, Arola D. Degradation in the fatigue crack growth resistance of human dentin by lactic acid. Mater Sci Eng C Mater Biol Appl. 2017;73:716–25.

    Article  Google Scholar 

  46. Ivancik J, Neerchal NK, Romberg E, Arola D. The reduction in fatigue crack growth resistance of dentin with depth. J Dent Res. 2011;90:1031–6.

    Article  Google Scholar 

  47. Goracci G, Mori G, Baldi M. Terminal end of the human odontoblast process: a study using SEM and confocal microscopy. Clin Oral Investig. 1999;3:126–32.

    Article  Google Scholar 

  48. Ito S, Saito T, Tay FR, Carvalho RM, Yoshiyama M, Pashley DH. Water content and apparent stiffness of non-caries versus caries-affected human dentin. J Biomed Mater Res B Appl Biomater. 2005;72:109–16.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Tjäderhane, L. (2019). Dentin Basic Structure, Composition, and Function. In: Versiani, M., Basrani, B., Sousa-Neto, M. (eds) The Root Canal Anatomy in Permanent Dentition. Springer, Cham. https://doi.org/10.1007/978-3-319-73444-6_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-73444-6_2

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-73443-9

  • Online ISBN: 978-3-319-73444-6

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics