Abstract
Exposure to pathogens, toxins, and pollutants is a major public health concern. Often air, water, and food are the major sources. Efforts to develop and deploy highly sensitive detection and diagnostic platforms are of great importance to protect the health of consumers, military personnel, and civilians. Biosensors employing a proper transducer have the potential to digitally amplify signals for prompt analysis of small amounts of analyte and the consequent institution of remedial actions. Cell-based biosensor (CBB) using mammalian and microbial cells is referred to as a functional biosensor since it interrogates the interaction between the living cells and an analyte to provide physiologically relevant information regarding the functionality of an analyte. Thus, CBB is advantageous over immunosensor or nucleic acid-based biosensors, which may not assess functionality. However, the major drawback of CBB is the maintenance of viability of the cells during deployment in a rugged environment or in the point-of-care use.
References
Ames BN, Durston WE, Yamasaki E, Lee FD (1973) Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci U S A 70(8):2281–2285
Aronoff-Spencer E, Venkatesh AG, Sun A, Brickner H, Looney D, Hall DA (2016) Detection of Hepatitis C core antibody by dual-affinity yeast chimera and smartphone-based electrochemical sensing. Biosens Bioelectron 86:690–696
Banerjee P, Bhunia AK (2009) Mammalian cell-based biosensors for pathogens and toxins. Trends Biotechnol 27(3):179–188
Banerjee P, Bhunia AK (2010) Cell-based biosensor for rapid screening of pathogens and toxins. Biosens Bioelectron 26:99–106
Banerjee P, Lenz D, Robinson JP, Rickus JL, Bhunia AK (2008) A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins. Lab Investig 88(2):196–206
Banerjee P, Franz B, Bhunia A (2010) Mammalian cell-based sensor system. In: Belkin S, Gu MB (eds) Whole cell sensing systems I, vol 117. Springer, Berlin, Germany, pp 21–55
Banerjee P, Kintzios S, Prabhakarpandian B (2013) Biotoxin detection using cell-based sensors. Toxins 5(12):2366–2383
Bhunia AK (2014) One day to one hour: how quickly can foodborne pathogens be detected? Future Microbiol 9(8):935–946
Bhunia AK, Wampler JL (2005) Animal and cell culture models for foodborne bacterial pathogens. In: Fratamico P, Bhunia AK, Smith JL (eds) Foodborne pathogens: microbiology and molecular biology, vol 1532. Caiser Academic Press, Taylor and Francis, Boca Raton, FL
Bhunia AK, Westbrook DG (1998) Alkaline phosphatase release assay to determine cytotoxicity for Listeria species. Lett Appl Microbiol 26:305–310
Biran A, Yagur-Kroll S, Pedahzur R, Buchinger S, Reifferscheid G, Ben-Yoav H, Shacham-Diamand Y, Belkin S (2010) Bacterial genotoxicity bioreporters. Microb Biotechnol 3(4):412–427
Cho I-H, Radadia AD, Farrokhzad K, Ximenes E, Bae E, Singh AK, Oliver H, Ladisch M, Bhunia A, Applegate B, Mauer L, Bashir R, Irudayaraj J (2014) Nano/micro and spectroscopic approaches to food pathogen detection. Annu Rev Anal Chem 7:65–88
Curtis T, Naal RMZG, Batt C, Tabb J, Holowka D (2008) Development of a mast cell-based biosensor. Biosens Bioelectron 23(7):1024–1031
Feng J, Jester BW, Tinberg CE, Mandell DJ, Antunes MS, Chari R, Morey KJ, Rios X, Medford JI, Church GM, Fields S, Baker D (2015) A general strategy to construct small molecule biosensors in eukaryotes. elife 4:e10606
Fu E, Yager P, Floriano PN, Christodoulides N, McDevitt JT (2011) Perspective on diagnostics for global health. IEEE Pulse 2(6):40–50
Jiang D, Jiang H, Ji J, Sun X, Qian H, Zhang G, Tang L (2014) Mast-cell-based fluorescence biosensor for rapid detection of major fish allergen parvalbumin. J Agric Food Chem 62(27):6473–6480
Jiang D, Zhu P, Jiang H, Ji J, Sun X, Gu W, Zhang G (2015) Fluorescent magnetic bead-based mast cell biosensor for electrochemical detection of allergens in foodstuffs. Biosens Bioelectron 70:482–490
Jiang D, Ge P, Wang L, Jiang H, Yang M, Yuan L, Ge Q, Fang W, Ju X (2019) A novel electrochemical mast cell-based paper biosensor for the rapid detection of milk allergen casein. Biosens Bioelectron 130:299–306
Johnstone AF, Gross GW, Weiss DG, Schroeder OH, Gramowski A, Shafer TJ (2010) Microelectrode arrays: a physiologically based neurotoxicity testing platform for the 21st century. Neurotoxicology 31(4):331–350
Kaina B, Fritz G (2006) DNA damaging agents encyclopedic reference of genomics and proteomics in molecular medicine. Springer, Berlin/Heidelberg, pp 416–423
Konowalchuk J, Speirs JI, Stavric S (1977) Vero response to a cytotoxin of Escherichia coli. Infect Immun 18(3):775–779
Maldonado Y, Fiser JC, Nakatsu CH, Bhunia AK (2005) Cytotoxicity potential and genotypic characterization of Escherichia coli isolates from environmental and food sources. Appl Environ Microbiol 71(4):1890–1898
Mavrikou S, Flampouri E, Iconomou D, Kintzios S (2017) Development of a cellular biosensor for the detection of aflatoxin B1, based on the interaction of membrane engineered Vero cells with anti-AFB1 antibodies on the surface of gold nanoparticle screen printed electrodes. Food Control 73:64–70
Mayer M, Arrizabalaga O, Lieb F, Ciba M, Ritter S, Thielemann C (2018) Electrophysiological investigation of human embryonic stem cell derived neurospheres using a novel spike detection algorithm. Biosens Bioelectron 100:462–468
Ngamwongsatit P, Banada PP, Panbangred W, Bhunia AK (2008) WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J Microbiol Methods 73(3):211–215
O’Shaughnessy TJ, Gray SA, Pancrazio JJ (2004) Cultured neuronal networks as environmental biosensors. J Appl Toxicol 24(5):379–385
Perdikaris A, Alexandropoulos N, Kintzios S (2009) Development of a novel, ultra-rapid biosensor for the qualitative detection of hepatitis B virus-associated antigens and anti-HBV, based on “membrane-engineered” fibroblast cells with virus-specific antibodies and antigens. Sensors (Basel, Switzerland) 9(3):2176–2186
Perdikaris A, Vassilakos N, Yiakoumettis I, Kektsidou O, Kintzios S (2011) Development of a portable, high throughput biosensor system for rapid plant virus detection. J Virol Methods 177(1):94–99
Qu M, Boruah BM, Zhang W, Li Y, Liu W, Bi Y, Gao GF, Yang R, Liu D, Gao B (2013) A Rat Basophilic Leukaemia cell sensor for the detection of pathogenic viruses. Biosens Bioelectron 43:412–418
Quiñones B, Massey S, Friedman M, Swimley MS, Teter K (2009) Novel cell-based method to detect Shiga toxin 2 from Escherichia coli O157:H7 and inhibitors of toxin activity. Appl Environ Microbiol 75(5):1410–1416
Rider TH, Petrovick MS, Nargi FE, Harper JD, Schwoebel ED, Mathews RH, Blanchard DJ, Bortolin LT, Young AM, Chen J, Hollis MA (2003) A B cell-based sensor for rapid identification of pathogens. Science 301(5630):213–215
Roberts PH, Davis KC, Garstka WR, Bhunia AK (2001) Lactate dehydrogenase release assay from Vero cells to distinguish verotoxin producing Escherichia coli from non-verotoxin producing strains. J Microbiol Methods 43(3):171–181
Salic A, Mitchison TJ (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 105(7):2415
Scarlatos A, Cadotte AJ, DeMarse TB, Welt BA (2008) Cortical networks grown on microelectrode arrays as a biosensor for botulinum toxin. J Food Sci 73(3):E129–E136
Shimomura-Shimizu M, Karube I (2010) Yeast based sensors. In: Belkin S, Gu MB (eds) Whole cell sensing systems I: reporter cells and devices. Springer, Berlin/Heidelberg, pp 1–19
Shroyer M, Bhunia A (2003) Development of a rapid 1-h fluorescence-based cytotoxicity assay for Listeria species. J Microbiol Methods 55(1):35–40
Singh AK, Bhunia AK (2018) Optical biosensors in foodborne pathogen detection smart biosensor technology, 2nd edn. Caister Academic Press, Norfolk, UK, p 443
Singh A, Poshtiban S, Evoy S (2013) Recent advances in bacteriophage based biosensors for food-borne pathogen detection. Sensors 13(2):1763–1786
Smartt AE, Ripp S (2011) Bacteriophage reporter technology for sensing and detecting microbial targets. Anal Bioanal Chem 400(4):991–1007
Stephenson L, Benedick J (2010) Cell-based assays. In Biofiles (Sauk Village, IL: Sigma-Aldrich)
Teo SC, Wong LS (2014) Whole cell-based biosensors for environmental heavy metals detection. Annu Res Rev Biol 4:2663–2674
To CZ, Bhunia AK (2019) Three dimensional Vero cell-platform for rapid and sensitive screening of Shiga-toxin producing Escherichia coli. Front Microbiol 10:949
Velusamy V, Arshak K, Korostynska O, Oliwa K, Adley C (2010) An overview of foodborne pathogen detection: in the perspective of biosensors. Biotechnol Adv 28(2):232–254
Wang X-Q, Duan X-M, Liu L-H, Fang Y-Q, Tan Y (2005) Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochim Biophys Sin 37(6):379–385
Wang L, Wang R, Kong B-W, Jin S, Ye K, Fang W, Li Y (2015) B cells using calcium signaling for specific and rapid detection of Escherichia coli O157: H7. Sci Rep 5:10598
Xin W, Yao W, Gao X, You Z, Gao S, Kang L, Li Q, Zhou Y, Yang H, Jiang P, Wang J (2014) Development of aequorin-based mast cell nanosensor for rapid identification of botulinum neurotoxin type B. J Biomed Nanotechnol 10(11):3318–3328
Xu Z, Mulchandani A, Chen W (2003) Detection of benzene, toluene, ethyl benzene, and xylenes (BTEX) using toluene dioxygenase-peroxidase coupling reactions. Biotechnol Prog 19(6):1812–1815
Xu T, Close D, Smartt A, Ripp S, Sayler G (2014) Detection of organic compounds with whole-cell bioluminescent bioassays. Adv Biochem Eng Biotechnol 144:111–151
Ye Y, Guo H, Sun X (2019) Recent progress on cell-based biosensors for analysis of food safety and quality control. Biosens Bioelectron 126:389–404
Zamani P, Sajedi RH, Hosseinkhani S, Zeinoddini M (2016) Hybridoma as a specific, sensitive, and ready to use sensing element: a rapid fluorescence assay for detection of Vibrio cholerae O1. Anal Bioanal Chem 408(23):6443–6451
Zhang D, Coronel-Aguilera CP, Romero PL, Perry L, Minocha U, Rosenfield C, Gehring AG, Paoli GC, Bhunia AK, Applegate B (2016) The use of a novel NanoLuc-based reporter phage for the detection of Escherichia coli O157:H7. Sci Rep 6:33235
Acknowledgments
This material is based upon work supported by the US Department of Agriculture; Agricultural Research Service, under Agreement No. 59-8072-6-001; the USDA National Institute of Food and Agriculture (Hatch accession no. 1016249); and the Center for Food Safety Engineering at Purdue University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Section Editor information
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this entry
Cite this entry
To, C., Banerjee, P., Bhunia, A.K. (2020). Cell-Based Biosensor for Rapid Screening of Pathogens and Toxins. In: Thouand, G. (eds) Handbook of Cell Biosensors. Springer, Cham. https://doi.org/10.1007/978-3-319-47405-2_102-1
Download citation
DOI: https://doi.org/10.1007/978-3-319-47405-2_102-1
Received:
Accepted:
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-47405-2
Online ISBN: 978-3-319-47405-2
eBook Packages: Springer Reference Chemistry and Mat. ScienceReference Module Physical and Materials ScienceReference Module Chemistry, Materials and Physics