Abstract
A sensitive diagnostic method to detect anti-Leishmania antibodies in urine samples has been developed. This simple method uses plastic plates coated with anti-human IgG. Urine samples are applied to the plate and then colored Leishmania donovani promastigotes are added serving as direct agglutination test (DAT) antigens. If the urine contains IgG to Leishmania, the DAT antigen attaches to the plate; sediment will be observed if there is no specific IgG. Among 56 urine samples from patients with visceral leishmaniasis (VL), 55 (98.2 %) were positive with this method. However, 13 endemic normals and 24 Japanese heathy controls were negative. As the results can be observed by the naked eye, no detection equipment is required, and only the antibody coated plate and DAT antigen which are stable at least for one year under ambient temperature , needed for the test. This method is considered useful for active surveys of visceral leishmaniasis at late stages of the control program.
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Acknowledgments
Research underlying this chapter, conducted by the authors, was partially supported by JST/JICA, SATREPS, and also by a Grant-in-Aid for Scientific Research (B) No. 18406013 from the Japan Society for the Promotion of Science.
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Nagaoka, F., Takagi, H., Noiri, E., Itoh, M. (2016). Antibody Capture Direct Agglutination Test (abcDAT) for Diagnosis of Visceral Leishmaniasis with Urine. In: Noiri, E., Jha, T. (eds) Kala Azar in South Asia. Springer, Cham. https://doi.org/10.1007/978-3-319-47101-3_13
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DOI: https://doi.org/10.1007/978-3-319-47101-3_13
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