Abstract
Brassica napus was formed through recent hybridization between Brassica oleracea and Brassica rapa and is an important source of edible oil. The genomic characterization of the families of genes involved in oil biosynthesis was undertaken in the B. napus genome assembly to assess the potential impact of selection breeding on gene content and function. We compared oil biosynthesis genes and found that the genes number has a huge difference in 14 different species. There are 2482 homologs in B. napus cv. ZS11 and only 120 homologs in Jatropha curcas. There is a 4.1 fold expansion over Arabidopsis thaliana and >20 fold expansion over J. curcas. However, the distributions of the gene number in the acyl metabolism pathway are highly similar in all the 14 species. The fatty acid elongation and wax biosynthesis pathway, the phospholipid signaling pathway, and the galactolipid, sulfolipid, and phospholipid synthesis pathway are the top pathways in terms of the gene number. A total of 19 positive selection genes were identified in B. napus. Among them, 5 genes are in the phospholipid signaling pathway and 5 genes in the triacylglycerol and fatty acid degradation pathway. These results will help better understand the mechanism and evolution of oil biosynthesis genes.
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References
Baud S, Lepiniec L (2009) Regulation of de novo fatty acid synthesis in maturing oilseeds of Arabidopsis. Plant Physiol Biochem. (PPB)/Societe francaise de physiologie vegetale 47:448–455
Baud S, Lepiniec L (2010) Physiological and developmental regulation of seed oil production. Prog Lipid Res 49:235–249
Baud S, Mendoza MS, To A, Harscoet E, Lepiniec L, Dubreucq B (2007) WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis. Plant J Cell Mol Biol 50:825–838
Cernac A, Benning C (2004) WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis. Plant J Cell Mol Biol 40:575–585
Chalhoub B, Denoeud F, Liu S, Parkin IA, Tang H, Wang X, Chiquet J, Belcram H, Tong C, Samans B, Correa M, Da Silva C, Just J, Falentin C, Koh CS, Le Clainche I, Bernard M, Bento P, Noel B, Labadie K, Alberti A, Charles M, Arnaud D, Guo H, Daviaud C, Alamery S, Jabbari K, Zhao M, Edger PP, Chelaifa H, Tack D, Lassalle G, Mestiri I, Schnel N, Le Paslier MC, Fan G, Renault V, Bayer PE, Golicz AA, Manoli S, Lee TH, Thi VH, Chalabi S, Hu Q, Fan C, Tollenaere R, Lu Y, Battail C, Shen J, Sidebottom CH, Wang X, Canaguier A, Chauveau A, Berard A, Deniot G, Guan M, Liu Z, Sun F, Lim YP, Lyons E, Town CD, Bancroft I, Wang X, Meng J, Ma J, Pires JC, King GJ, Brunel D, Delourme R, Renard M, Aury JM, Adams KL, Batley J, Snowdon RJ, Tost J, Edwards D, Zhou Y, Hua W, Sharpe AG, Paterson AH, Guan C, Wincker P (2014) Plant genetics. Early allopolyploid evolution in the post-neolithic Brassica napus oilseed genome. Science 345:950–953
Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandager L, Ronne H, Stymne S (2000) Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97:6487–6492
Goffman FD, Alonso AP, Schwender J, Shachar-Hill Y, Ohlrogge JB (2005) Light enables a very high efficiency of carbon storage in developing embryos of rapeseed. Plant Physiol 138:2269–2279
Hasan M, Friedt W, Pons-Kuhnemann J, Freitag NM, Link K, Snowdon RJ (2008) Association of gene-linked SSR markers to seed glucosinolate content in oilseed rape (Brassica napus ssp. napus). TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 116:1035–1049
Hu ZY, Hua W, Zhang L, Deng LB, Wang XF, Liu GH, Hao WJ, Wang HZ (2013) Seed structure characteristics to form ultrahigh oil content in rapeseed. PLoS ONE 8:e62099
Hua W, Li RJ, Zhan GM, Liu J, Li J, Wang XF, Liu GH, Wang HZ (2012) Maternal control of seed oil content in Brassica napus: the role of silique wall photosynthesis. Plant J Cell Mol Biol 69:432–444
Mu J, Tan H, Zheng Q, Fu F, Liang Y, Zhang J, Yang X, Wang T, Chong K, Wang XJ, Zuo J (2008) LEAFY COTYLEDON1 is a key regulator of fatty acid biosynthesis in Arabidopsis. Plant Physiol 148:1042–1054
Pouvreau B, Baud S, Vernoud V, Morin V, Py C, Gendrot G, Pichon JP, Rouster J, Paul W, Rogowsky PM (2011) Duplicate maize Wrinkled1 transcription factors activate target genes involved in seed oil biosynthesis. Plant Physiol 156:674–686
Ruuska SA, Schwender J, Ohlrogge JB (2004) The capacity of green oilseeds to utilize photosynthesis to drive biosynthetic processes. Plant Physiol 136:2700–2709
Sakhno LA (2010) Fatty acid composition variability of rapeseed oil: classical selection and biotechnology. Tsitol Genet 44:70–80
Santos-Mendoza M, Dubreucq B, Baud S, Parcy F, Caboche M, Lepiniec L (2008) Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis. Plant J Cell Mol Biol 54:608–620
Schwender J, Ohlrogge JB, Shachar-Hill Y (2003) A flux model of glycolysis and the oxidative pentosephosphate pathway in developing Brassica napus embryos. J Biol Chem 278:29442–29453
Schwender J, Shachar-Hill Y, Ohlrogge JB (2006) Mitochondrial metabolism in developing embryos of Brassica napus. J Biol Chem 281:34040–34047
Schwender J, Goffman F, Ohlrogge JB, Shachar-Hill Y (2004) Rubisco without the Calvin cycle improves the carbon efficiency of developing green seeds. Nature 432:779–782
Snyder CL, Yurchenko OP, Siloto RM, Chen X, Liu Q, Mietkiewska E, Weselake RJ (2009) Acyltransferase action in the modification of seed oil biosynthesis. New Biotechnol 26:11–16
Stahl U, Carlsson AS, Lenman M, Dahlqvist A, Huang B, Banas W, Banas A, Stymne S (2004) Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiol 135:1324–1335
Sun F, Fan G, Hu Q, Zhou Y, Guan M, Tong C, Li J, Du D, Qi C, Jiang L, Liu W, Huang S, Chen W, Yu J, Mei D, Meng J, Zeng P, Shi J, Liu K, Wang X, Wang X, Long Y, Liang X, Hu Z, Huang G, Dong C, Zhang H, Li J, Zhang Y, Li L, Shi C, Wang J, Lee SM, Guan C, Xu X, Liu S, Liu X, Chalhoub B, Hua W, Wang H (2017) The high-quality genome of Brassica napus cultivar ‘ZS11’ reveals the introgression history in semi-winter morphotype. Plant J Cell Mol Biology 92:452–468
Wang H, Guo J, Lambert KN, Lin Y (2007) Developmental control of Arabidopsis seed oil biosynthesis. Planta 226:773–783
Weselake RJ, Taylor DC, Rahman MH, Shah S, Laroche A, McVetty PB, Harwood JL (2009) Increasing the flow of carbon into seed oil. Biotechnol Adv 27:866–878
Yurchenko OP, Weselake RJ (2011) Involvement of low molecular mass soluble acyl-CoA-binding protein in seed oil biosynthesis. New Biotechnol 28:97–109
Acknowledgements
This work was supported by the National 863 Plan projects (2013AA102602 and 2012AA101107), National Key Basic Research Program of China (2015CB150200), the Industry Technology System of Rapeseed in China (CARS-12), and the Hubei Agricultural Science and Technology Innovation Center of China.
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Hu, Z., Hua, W. (2018). Case Study for Trait-Related Gene Evolution: Oil Biosynthesis Genes. In: Liu, S., Snowdon, R., Chalhoub, B. (eds) The Brassica napus Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-43694-4_11
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DOI: https://doi.org/10.1007/978-3-319-43694-4_11
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