Skip to main content
SpringerLink
Log in

Intracytoplasmic Morphologically Selected Sperm Injection

  • Chapter
  • First Online:
In Vitro Fertilization

Abstract

Intracytoplasmic morphologically selected sperm injection (IMSI) provides the opportunity to deselect morphologically abnormal spermatozoa presenting large vacuole-like structures (VLS), decreasing the risk of birth defects. According to the growing body of literature, VLS most likely reflect anomalies of sperm chromatin packaging rendering the DNA, at least in part, more vulnerable to reactive oxygen species (ROS). The importance of repackaging of the haploid genome before delivery in the oocyte is a crucial step. It is reported that VLS are related to alteration in the methylation pattern (hypermethylation) secondary to chromatin condensation failures. All these potential epigenetic pattern disturbances may represent the basis of numerous disorders in the offspring as well as for the future generation.

Debates and skepticism still exist about the efficiency of IMSI, mainly due to a low number of controlled randomized studies published yet. IMSI is indeed important: in women with advanced maternal age, possibly due to reduced correction and reparation capacity of spermatozoon defects in presence of aging oocyte, and in patients with failure of embryo development to the blastocyst stage in a previous in vitro fertilization (IVF) attempt.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Vawda A, Gunby J, Younglai E. Semen parameters as predictors of in-vitro fertilization: the importance of strict criteria morphology. Hum Reprod. 1996;11:1445–50.

    Article  CAS  PubMed  Google Scholar 

  2. Nikolettos N, Kiipker W, Demirel C, Schopper B, Blasig C, Sturm R, Felberbaum R, Bauer O, Diedrich K, Al-Hasani S. Fertilization potential of spermatozoa with abnormal morphology. Hum Reprod. 1999;14(Suppl. 1):47–70.

    Article  PubMed  Google Scholar 

  3. De Vos A, Van De Velde H, Joris H, Verheyen G, Devroey P, Van Steirteghem A. Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil Steril. 2003;79:42–8.

    Article  PubMed  Google Scholar 

  4. Garolla A, Fortini D, Menegazzo M, De Toni L, Nicoletti V, Moretti A, Selice R, Engl B, Foresta C. High power magnification microscopy and functional status analysis of sperm in the evaluation and selection before ICSI. Reprod Biomed Online. 2008;17:610–6.

    Article  PubMed  Google Scholar 

  5. Bartoov B, Berkovitz A, Eltes F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001;345:1067–8.

    Article  CAS  PubMed  Google Scholar 

  6. Coppola G, Di Caprio G, Wilding M, Ferraro P, Esposito G, Di Matteo L, Dale R, Coppola G, Dale B. Digital holographic microscopy for the evaluation of human sperm structure. Zygote. 2013:1–9.

    Google Scholar 

  7. Boitrelle F, Ferfouri F, Petit JM, Segretain D, Tourain C, Bergere M, Bailly M, Vialard F, Albert M, Selva J. Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod. 2011;26:1650–8.

    Article  CAS  PubMed  Google Scholar 

  8. Thomas Huser T, Orme C, Hollars CW, Corzett M, Balhorn R. Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. J Biophotonics. 2009;2:322–32.

    Article  PubMed  CAS  Google Scholar 

  9. Saidi R, Rives N, Gruel E, Mazurier S, Mousset-Simeon N, Mace B. New classification of spermatocytogram at high magnification. Med Repro Gyn Endo. 2008;10:315–24.

    Google Scholar 

  10. Perdrix A, Saïdi R, Ménard JF, Gruel E, Milazzo JP, Macé B, Rives N. Relationship between conventional sperm parameters and motile sperm organelle morphology examination (MSOME). Int J Androl. 2012;35:491–8.

    Article  CAS  PubMed  Google Scholar 

  11. Franco J, Baruffi R, Mauri A, Petersen C, Oliveira J, Vagnini L. Significance of large nuclear vacuoles in human spermatozoa: implications for ICSI. Reprod Biomed Online. 2008;17:42–5.

    Article  PubMed  Google Scholar 

  12. Vanderzwalmen P, Bach M, Baramsai B, Neyer A, Schwerda D, Stecher A, Wirleitner B, Zintz M, Lejeune B, Vanderzwalmen S, Cassuto G, Zech M, Zech NH. Intracytoplasmic Morphologically selected sperm injection. In: Nagy ZP, et al., editors. Practical manual of in vitro fertilization: advanced methods and Novel devices. Springer-Verlag New York: Springer Science; 2012. p. 229–40.

    Chapter  Google Scholar 

  13. Cassuto NG, Bouret D, Plouchart JM, Jellad S, Vanderzwalmen P, Balet R, Larue L, Barak Y. A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril. 2009;92:1616–25.

    Article  PubMed  Google Scholar 

  14. Auger J, Eustache F, Andersen A, Irvine D, Jørgensen N, Skakkebaek N, Suominen J, Toppari J, Vierula M, Jouannet P. Sperm morphological defects related to environment, lifestyle and medical history of 1001 male partners of pregnant women from four European cities. Hum Reprod. 2011;16:2710–7.

    Article  Google Scholar 

  15. Westbrook VA, Diekman AB, Klotz KL, Khole VV, von Kap-Herr C, Golden WL, Eddy RL, Shows TB, Stoler MH, Lee CY, Flickinger CJ, Herr JC. Spermatid-specific expression of the novel X-linked gene product SPAN-X localized to the nucleus of human spermatozoa. Biol Reprod. 2000;63:469–81.

    Article  CAS  PubMed  Google Scholar 

  16. Watanabe S, Tanaka A, Fujii S, Mizunuma H. No relationship between chromosome aberrations and vacuole-like structures on human sperm head. Hum Reprod. 2009;24:i96.

    Article  CAS  Google Scholar 

  17. Chemes HE, Alvarez SC. Tales of the tail and sperm head aches: changing concepts on the prognostic significance of sperm pathologies affecting the head, neck and tail. Asian J Androl. 2012;141:14–23.

    Article  Google Scholar 

  18. Auger J, Dadoune JP. Nuclear status of human sperm cells by transmission Electron microscopy and image cytometry: changes in nuclear shape and chromatin texture during Spermiogenesis and Epididymal transit. Biol Reprod. 1993;49:166–75.

    Article  CAS  PubMed  Google Scholar 

  19. Ward WS. Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod. 2010;16:30–6.

    Article  CAS  PubMed  Google Scholar 

  20. Zheng H, Stratton C, Morozumi K, Jin J, Yanagimachi R, Yan W. Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and male infertility. PNAS. 2007;104:6852–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Rousseaux S, Boussouar F, Gaucher J, Reynoird N, Montellier E, Curtet S, Vitte AL, Khochbin S. Molecular models for post-meiotic male genome reprogramming. Syst Biol Reprod Med. 2011;57:50–3.

    Article  PubMed  Google Scholar 

  22. Montellier E, Boussouar F, Rousseaux S, Zhang K, Buchou T, Fenaille F, Shiota H, Debernardi A, Héry P, Curtet S, Jamshidikia M, Barral S, Holota H, Bergon A, Lopez F, Guardiola P, Pernet K, Imbert J, Petosa C, Tan M, Zhao Y, Gérard M, Khochbin S. Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B. Genes Dev. 2013;27:1680–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Miller D, Brinkworth M, Iles D. Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics. Reproduction. 2010;139:287–301.

    Article  CAS  PubMed  Google Scholar 

  24. Tavalaee M, Razavi S, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril. 2009;91:1119–26.

    Article  CAS  PubMed  Google Scholar 

  25. Oliva R, Ballesca JL. Altered histone retention and epigenetic modifications in the sperm of infertile men. Asian J Androl. 2012;14:239–40.

    Article  CAS  PubMed  Google Scholar 

  26. Hammoud SS, Nix DA, Hammoud AO, Gibson M, Cairns BR, Carrell DT. Genome-wide analysis identifies changes in histone retention and epigenetic modifications at developmental and imprinted gene loci in the sperm of infertile men. Hum Reprod. 2011;26:2558–69.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Berkovitz A, Eltes F, Yaari S, Katz N, Barr I, Fishman A, Bartoov B. The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod. 2005;20:185–90.

    Article  PubMed  Google Scholar 

  28. Hazout A, Dumont-Hassan M, Junca AM, Cohen Bacrie P, Tesarik J. High-magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online. 2006;12:19–25.

    Article  PubMed  Google Scholar 

  29. Tanaka A, Nagayoshi M, Tanaka I, Kuzunoki H. Human sperm head vacuoles are physiological structures formed during the sperm development and maturation process. Fertil Stertil. 2012;98:315–20.

    Article  Google Scholar 

  30. Mundy AJ, Ryder TA, Edmonds DK. A quantitative study of sperm head ultrastructure in subfertile males with excess sperm precursors. Fertil Steril. 1994;61:751–4.

    Article  CAS  PubMed  Google Scholar 

  31. Sakkas D, Juan G, Alvarez JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril. 2010;93:1027–36.

    Article  CAS  PubMed  Google Scholar 

  32. Mahfouz R, Sharma R, Thiyagarajan A, Kale V, Gupta S, Sabanegh E, Agarwal A. Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species. Fertil Steril. 2010;94:2141–6.

    Article  CAS  PubMed  Google Scholar 

  33. Perdrix A, Travers A, Chelli MH, Escalier D, Do Rego JL, Milazzo JP, Mousset-Siméon N, Macé B, Rives N. Assessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles. Hum Reprod. 2011;26:47–58.

    Article  CAS  PubMed  Google Scholar 

  34. Cassuto NG, Hazout A, Hammoud I, Balet R, Bouret D, Barak Y, Jellad S, Plouchart JM, Selva J, Yazbeck C. Correlation between DNA defect and sperm-head morphology. Reprod Biomed Online. 2012;24:211–8.

    Article  PubMed  Google Scholar 

  35. Franco JG Jr, Mauri AL, Petersen CG, Massaro FC, Silva LF, Felipe V, Cavagna M, Pontes A, Baruffi RL, Oliveira JB, Vagnini LD. Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl. 2012;35:46–51.

    Article  PubMed  Google Scholar 

  36. Boitrelle F, Albert M, Petit J-M, Ferfouri F, Wainer R, Bergere M, Bailly M, Vialard F, Selva J. Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure. Reprod Biomed Online. 2013;27:201–11.

    Article  CAS  PubMed  Google Scholar 

  37. Wilding M, Coppola G, di Matteo L, Palagiano A, Fusco E, Dale B. Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet. 2011;28:253–62.

    Article  PubMed  Google Scholar 

  38. Hammoud I, Boitrelle F, Ferfouri F, Vialard F, Bergere M, Wainer B, Bailly M, Albert M, Selva J. Selection of normal spermatozoa with a vacuole-free head (×6300) improves selection of spermatozoa with intact DNA in patients with high sperm DNA fragmentation rates. Andrologia. 2013;45:163–70.

    Article  CAS  PubMed  Google Scholar 

  39. Cayli S, Jakab A, Ovari L, Delpiano E, Celik-Ozenci C, Sakkas D, Ward D, Huszar G. Biochemical markers of sperm function: male fertility and sperm selection for ICSI. Reprod Biomed Online. 2003;7:462–8.

    Article  CAS  PubMed  Google Scholar 

  40. Perdrix A, Travers A, Clatot F, Sibert L, Mitchell V, Jumeau F, Macé B, Rives N. Modification of chromosomal architecture in human spermatozoa with large vacuoles. Andrology. 2013;1:57–66.

    Article  CAS  PubMed  Google Scholar 

  41. Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. 2004;81:1289–95.

    Article  PubMed  Google Scholar 

  42. Hammadeh M, Nkemayim D, Georg T, et al. Sperm morphology and chromatin condensation before and after semen processing. Arch Androl. 2000;44:221–6.

    Article  CAS  PubMed  Google Scholar 

  43. Gopalkrishnan K, Padwal V, Meherji PK, et al. Poor quality of sperm as it affects repeated early pregnancy loss. Arch Androl. 2000;45:111–7.

    Article  CAS  PubMed  Google Scholar 

  44. Aitken RJ, De Iuliis GN. Origins and consequences of DNA damage in male germ cells. Reprod Biomed Online. 2007;14:727–33.

    Article  CAS  PubMed  Google Scholar 

  45. Tesarik J. MSOME and sperm DNA integrity: Biological and clinical consideration. In: Agarwal, et al., editors. Non-invasive sperm selection for in vitro fertilization: Novel concepts and methods. New York: Springer Science + Business Media; 2015. p. 137–47.

    Google Scholar 

  46. Kumar M, Kumar K, Jain S, Hassan T, Dada R. Novel insights into the genetic and epigenetic paternal contribution to the human embryo. Clinics. 2013;68(1):5–14.

    Article  PubMed  PubMed Central  Google Scholar 

  47. Shaoqin G, Zhenghui Z, Xueqian Z, Yuan H. Epigenetic modifications in human spermatozoon and its potential role in embryonic development. Yi Chuan. 2014;36:439–46.

    PubMed  Google Scholar 

  48. Schagdarsurengin U, Paradowska A, Steger K. Analysing the sperm epigenome: roles in early embryogenesis and assisted reproduction. Nat Rev Urol. 2012;9:609–19.

    Article  CAS  PubMed  Google Scholar 

  49. Feinberg J, Bakulski K, Jaffe A, Tryggvadottir R, Brown S, Goldman L, Croen L, Hertz-Picciotto I, Newschaffer C, Fallin M, Feinberg A. Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort. Int J Epidemiol. 2015;44:1199–210.

    Article  PubMed  PubMed Central  Google Scholar 

  50. Kobayashi H, Hiura H, John R, Sato A, Otsu E, Kobayashi N, Suzuki R, Suzuki F, Hayashi C, Utsunomiya T, Yaegashi N, Arima T. DNA methylation errors at imprinted loci after assisted conception originate in the parental sperm. Eur J Hum Genet. 2009;17:1582–91.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Montjean D, De La Grange P, Gentien D, Rapinat A, Belloc S, Cohen-Bacrie P, Menezo Y, Benkhalifa M. Sperm transcriptome profiling in oligozoospermia. J Assist Reprod Genet. 2012;29(1):3–10.

    Article  PubMed  Google Scholar 

  52. Marques CJ, Carvalho F, Sousa M, Barros A. Genomic imprinting in disruptive spermatogenesis. Lancet. 2004;363(9422):1700–2.

    Article  CAS  PubMed  Google Scholar 

  53. Houshdaran S, Cortessis VK, Siegmund K, Yang A, Laird PW, Sokol RZ. Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm. PLoS ONE. 2007;2(12):e1289.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  54. Barzideh J, Scott RJ, Aitken RJ. Analysis of the global methylation status of human spermatozoa and its association with the tendency of these cells to enter apoptosis. Andrologia. 2013;45:424–9.

    Article  CAS  PubMed  Google Scholar 

  55. Filipponi D, Feil R. Perturbation of genomic imprinting in oligozoospermia. Epigenetics. 2009;4(1):27–30.

    Article  CAS  PubMed  Google Scholar 

  56. Rajender S, Avery K, Agarwal A. Epigenetics, spermatogenesis and male infertility. Mutat Res. 2011;727(3):62–71.

    Article  CAS  PubMed  Google Scholar 

  57. Cassuto G, Montjean D, Siffroi JP, Bouret D, Marzouk F, Copin H, Benkhalifa M. Different levels of DNA methylation detected in human sperms after morphological selection using high magnification microscopy. Biomed Res Int. 2016;2016:1–7. https://doi.org/10.1155/2016/6372171.

    Article  CAS  Google Scholar 

  58. Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, Artzi S, Gross M, Barak Y. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003;80:1413–9.

    Article  PubMed  Google Scholar 

  59. Vanderzwalmen P, Hiemer A, Rubner P, Bach M, Neyer A, Stecher A, Uher P, Zintz M, Lejeune B, Vanderzwalmen S, Cassuto G, Zech NH. Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online. 2008;17:617–27.

    Article  PubMed  Google Scholar 

  60. Cassuto NG, Bouret D, Plouchart JM, Jellad S, Vanderzwalmen P, Balet R, Larue L, Barak Y. A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril. 2009;92:1616–25.

    Article  PubMed  Google Scholar 

  61. Knez K, Tomazevic T, Vrtacnik-Bokal E, Virant-Klun I. Developmental dynamics of IMSI-derived embryos: a time-lapse prospective study. Reprod Biomed Online. 2013;27(2):161–71.

    Article  PubMed  Google Scholar 

  62. Knez K, Zorn B, Tomazevic T, Vrtacnik-Bokal E, Virant-Klun I. The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: a comparative prospective randomized study. Reprod Biol Endocrinol. 2011;9:123.

    Article  PubMed  PubMed Central  Google Scholar 

  63. Neyer A, Zintz M, Stecher A, Bach M, Wirleitner B, Zech NH, Vanderzwalmen P. The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging-a retrospective observational study. J Assist Reprod Genet. 2015;32:1607–14.

    Article  PubMed  PubMed Central  Google Scholar 

  64. Simon L, Murphy K, Shamsi M, Liu L, Emery B, Aston K, Hotaling J, Carell D. Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod. 2014;29:2402–12.

    Article  CAS  PubMed  Google Scholar 

  65. Shoukir Y, Chardonnens D, Campana A, Sakkas D. Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence? Hum Reprod. 1998;13:1632–7.

    Article  CAS  PubMed  Google Scholar 

  66. Tesarik J, Greco E, Mendoza C. Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation. Hum Reprod. 2004;19:611–5.

    Article  CAS  PubMed  Google Scholar 

  67. Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y. Real-time fine morphology of motile human sperm cell is associated with IVF-ICSI outcome. J Androl. 2002;23:1–8.

    Article  PubMed  Google Scholar 

  68. Knez K, Zorn B, Tomazevic T, Vrtacnik–Bokal E, Virant-Klun I. The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: A comparative prospective randomized study. Reprod Biol Endocrinol. 2011;9:123–30.

    Article  PubMed  PubMed Central  Google Scholar 

  69. Larson KL, DeJonge CJ, Barnes AM, Jost LK, Evenson DP. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod. 2000;15:1717–22.

    Article  CAS  PubMed  Google Scholar 

  70. Nasr-Esfahani MH, Salehi M, Razavi S, Anjomshoa M, Rozbahani S, Moulavi F, Mardani M. Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI. Reprod Biomed Online. 2005;11:198–205.

    Article  CAS  PubMed  Google Scholar 

  71. Berkovitz A, Eltes F, Paul M. The chance of having a healthy normal child following intracytoplasmic morphologically-selected sperm injection (IMSI) treatment is higher compared to conventional IVF-ICSI treatment. Fertil Steril. 2007;88:S20.

    Article  Google Scholar 

  72. Cassuto NG, Hazout A, Bouret D, Balet R, Larue L, Benifla JL, Viot G. Low birth defects by deselecting abnormal spermatozoa before ICSI. Reprod Biomed Online. 2014;28:47–53.

    Article  PubMed  Google Scholar 

  73. Baccetti B, Burrini AG, Collodel G, Magnano AR, Piomboni P, Renieri T, Sensini C. Crater defect in human spermatozoa. Mol Reprod Develop. 1989;22:249–55.

    CAS  Google Scholar 

  74. Clermont Y. The cycle of the seminiferous epithelium in man. Am J Anat. 1963;112:35–51.

    Article  CAS  PubMed  Google Scholar 

  75. De Vos A, Van de Velde H, Bocken G, Eylenbosch G, Franceus N, Meersdom G, Tistaert S, Vankelecom A, Tournaye H, Verheyen G. Does intracytoplasmic morphologically selected sperm injection improve embryo development? A randomized sibling-oocyte study. Hum Reprod. 2013;28:617–26.

    Article  PubMed  CAS  Google Scholar 

  76. Silva L, Oliveira J, Petersen C, Mauri A, Massaro F, Cavagna M, Baruffi RL, Franco JG Jr. The effects of male age on sperm analysis by motile sperm organelle morphology examination (MSOME). Reprod Biol Endocrinol. 2012;10:19.

    Article  PubMed  PubMed Central  Google Scholar 

  77. De Almeida Ferreira Braga DP, Setti AS, Figueira RC, Nichi M, Martinhago CD, Iaconelli A Jr, Borges E Jr. Sperm organelle morphologic abnormalities: contributing factors and effects on intracytoplasmic sperm injection cycles outcomes. Urology. 2011;78:786–91.

    Article  PubMed  Google Scholar 

  78. Kacem O, Sifer C, Barraud-Lange V, Ducot B, De Ziegler D, Poirot C, Wolf J. Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin. Reprod Biomed Online. 2010;20:132–7.

    Article  CAS  PubMed  Google Scholar 

  79. Montjean D, Belloc S, Benkhalifa M, Dalleac A, Ménézo Y. Sperm vacuoles are linked to capacitation and acrosomal status. Hum Reprod 2012; 27:2927–32.

    Article  CAS  PubMed  Google Scholar 

  80. Gatimel N, Léandri RD, Foliguet B, Bujan L, Parinaud J. Sperm cephalic vacuoles: new arguments for their non acrosomal origin in two cases of total globozoospermia. Andrology. 2013;1:52–6.

    Article  CAS  PubMed  Google Scholar 

  81. Neyer A, Vanderzwalmen P, Bach M, Stecher A, Spitzer D, Zech N. Sperm head vacuoles are not affected by in-vitro conditions, as analysed by a system of sperm-microcapture channels. Reprod Biomed Online. 2013;26:368–77.

    Article  CAS  PubMed  Google Scholar 

  82. Perdrix A, Rives N. Motile sperm organelle morphology examination (MSOME) and sperm head vacuoles: state of the art in. Hum Reprod Update. 2013;19:527–41.

    Article  PubMed  Google Scholar 

  83. Berkovitz A, Eltes F, Lederman H, Peer S, Ellenbogen A, Feldberg B, Bartoov B. How to improve IVF-ICSI outcome by sperm injection? Reprod Biomed Online. 2006;12:634–8.

    Article  CAS  PubMed  Google Scholar 

  84. Greco E, Scarselli F, Fabozzi G, Colasante A, Zavaglia D, Alviggi E, Litwicka K, Varricchio MT, Minasi M, Tesarik J. Sperm vacuoles negatively affect outcomes in intracytoplasmic morphologically selected sperm injection in terms of pregnancy, implantation, and live-birth rates. Fertil. Steril. 2013;100:379–85.

    Article  PubMed  Google Scholar 

  85. Antinori M, Licata E, Dani G, Cerusico F, Versaci C, D'Angelo D, Antinori S. Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online. 2008;16:835–41.

    Article  PubMed  Google Scholar 

  86. Nadalini M, Tarozzi N, Distratis V, Scaravelli G, Borini A. Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome. Reprod Biomed Online. 2009;19:45–55.

    Article  PubMed  Google Scholar 

  87. Setti SA, Ferreira RC, Paes de Almeida Ferreira Braga D de Cassia Savio Figueira Rerreira Iaconelli A Jr., Borges E Jr. Intracytoplasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod Biomed Online 2010;21:450–455.

    Article  Google Scholar 

  88. Delaroche L, Yazbeck C, Gout C, Kahn V, Oger P, Rougier N. Intracytoplasmic morphologically selected sperm injection (IMSI) after repeated IVF or ICSI failures: a prospective comparative study. Eur J Obstet Gynecol Reprod Biol. 2013;167:76–80.

    Article  PubMed  Google Scholar 

  89. Mauri A, Petersen C, Oliveira J, Massaro F, Baruffi R, Franco JG Jr. Comparison of day 2 embryo quality after conventional ICSI versus intracytoplasmic morphologically selected sperm injection (IMSI) using sibling oocytes. Eur J Obstet Gynecol Reprod Biol. 2010;150:42–6.

    Article  PubMed  Google Scholar 

  90. Leandri RD, Gachet A, Pfeffer J, Celebi C, Rives N, Carre-Pigeon F, Kulski O, Mitchell V, Parinaud J. Is intracytoplasmic morphologically selected sperm injection (IMSI) beneficial in the first ART cycle? A multicentric randomized controlled trial. Andrology. 2013;5:692–7.

    Article  Google Scholar 

  91. Dam AH, Feenstra I, Westphal JR, Ramos L, van Golde RJ, Kremer JA. Globozoospermia revisited. Hum Reprod Update. 2007;13:63–75.

    Article  CAS  PubMed  Google Scholar 

  92. Cox L, Larman MG, Saunders CM, Hashimoto K, Swann K, Lai FA. Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes. Reproduction. 2002;124:611–23.

    Article  CAS  PubMed  Google Scholar 

  93. Kashir J, Heindryckx B, Jones C, De Sutter P, Parrington J, Coward K. Oocyte activation, phospholipase C zeta and human infertility. Hum Reprod Update. 2010;16:690–703.

    Article  CAS  PubMed  Google Scholar 

  94. Montag M, Köster M, van der Ven K, Bohlen U, van der Ven H. The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle. Reprod Biomed Online. 2012;24(5):521–6.

    Article  PubMed  Google Scholar 

  95. Stecher A, Bach M, Neyer A, Vanderzwalmen P, Zintz M, Zech NH. Case report: live birth following ICSI with non-vital frozen-thawed testicular sperm and oocyte activation with calcium ionophore. J Assist Reprod Genet. 2011;28:411–4.

    Article  PubMed  PubMed Central  Google Scholar 

  96. Kashir J, Sermondade N, Sifer C, Lin Oo S, Jones C, Mounce G, Turner K, Child T, McVeigh E, Coward K. Motile sperm organelle morphology evaluation-selected globozoospermic human sperm with an acrosomal bud exhibits novel patterns and higher levels of phospholipase C zeta. Human Reprod. 2012;27(11):3150–60.

    Article  CAS  Google Scholar 

  97. Sermondade N, Hafhouf E, Dupont C, Bechoua S, Palacios C, Eustache F, Poncelet C, Benzacken B, Le´vy R, Sifer C. Successful childbirth after intracytoplasmic morphologically selected sperm injection without assisted oocyte activation in a patient with globozoospermia. Hum Reprod. 2011;26:2944–9.

    Article  CAS  PubMed  Google Scholar 

  98. Setti A, Figueira R, Braga D, Aoki T, Iaconelli A, Borges E. Intracytoplasmic morphologically selected sperm injection is beneficial in cases of advanced maternal age: a prospective randomized study. Eur J Obs Gynec Reprod Biol. 2013;171:286–90.

    Article  CAS  Google Scholar 

  99. Ménézo Y, Dale B, Cohen M. DNA damage and repair in human oocytes and embryos: a review. Zygote. 2010;18:357–65.

    Article  PubMed  CAS  Google Scholar 

  100. Brandriff B, Pedersen RA. Repair of the ultraviolet-irradiated male genome in fertilized mouse eggs. Science. 1981;211:1431–3.

    Article  CAS  PubMed  Google Scholar 

  101. Gunes S, Al-Sadaan M, Spermatogenesis AA. DNA damage and DNA repair mechanisms in male infertility. Reprod Biomed Online. 2015;31:309–19.

    Article  CAS  PubMed  Google Scholar 

  102. Kobayashi H, Sato A, Otsu E, et al. Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum Mol Genet. 2007;16:2542–51.

    Article  CAS  PubMed  Google Scholar 

  103. Peer S, Eltes F, Berkovitz A, Yehuda R, Itsykson P, Bartoov B. Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37 degrees C°? Fertil Steril. 2007;88:1589–94.

    Article  PubMed  Google Scholar 

  104. Vanderzwalmen P, Zech N, Lejeune B, Neyer A, Perrier d’´Hauterive S, Puissant F, Stecher A, Vanderzwalmen S, Wirleitner B, Gaspard O. Sperm vacuoles: Origin and implications. In: Agarwal A, et al., editors. Non-invasive sperm selection for in vitro fertilization: Novel concepts and methods. New York: Springer Science + Business media; 2015. p. 111–21.

    Google Scholar 

  105. Schwarz C, Köster M, van der Ven K, Montag M. Temperature-induced sperm nuclear vacuolization is dependent on sperm preparation. Andrologia. 2012;44(Suppl 1):126–9.

    Article  PubMed  Google Scholar 

  106. Vanderzwalmen P, Bach M, Gaspard O, Lejeune B, Neyer A, Puissant F, Schuff M, Stecher A, Vanderzwalmen S, Wirleitner B, Zech NH. Morphological selection of gametes and embryos: Sperm. In: Montag M, editor. A practical guide to selecting gametes and embryos. United Kingdom: Cambridge University Press; 2014. p. 59–79.

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. IVF Center CHIREC, Braine l’Alleud-Waterloo, Belgium

    Pierre Vanderzwalmen, Romain Imbert, David Jareno Martinez, Anne Vansteenbrugge & Sabine Vanderzwalmen

  2. NextClinic IVF Centers. Prof. Zech, Bregenz, Austria

    Pierre Vanderzwalmen, Astrid Stecher & Maximillian Murtinger

  3. IVF Centers Prof. Zech, IVF Laboratory, Bregenz, Austria

    Barbara Wirleitner

Authors
  1. Pierre Vanderzwalmen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Romain Imbert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Jareno Martinez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Astrid Stecher
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anne Vansteenbrugge
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sabine Vanderzwalmen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Barbara Wirleitner
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Maximillian Murtinger
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Reproductive Biology Associates – Prelude, Atlanta, GA, USA

    Zsolt Peter Nagy

  2. Astra Fertility Group, Mississauga, ON, Canada

    Alex C. Varghese

  3. Cleveland Clinic, Cleveland, OH, USA

    Ashok Agarwal

Review Questions

Review Questions

  1. 1.

    You want to implement a low cost IMSI station: Which equipment is mandatory to observe precisely and easily spermatozoa with small or large vacuoles?

    High magnification system

    or

    Nomarski differential interference contrast (DIC) optics

  1. 2.

    Why should spermatozoa with large VLS be deselected for injection?

  2. 3.

    Why is repackaging of the haploid genome before delivery in the oocyte a crucial step?

  3. 4.

    In which situations is it better to apply IMSI?

    Previous attempt with no fertilization

    Previous attempt with fragmented embryo development

    Woman aged older than 38 years

  1. 5.

    You have to face the following situations: woman with 15 follicles and man with one million spermatozoa. How will you organize chronologically your work?

    Oocyte retrieval – sperm preparation – dish preparation – sperm selection and isolation of the best-looking spermatozoa – IMSI

    or

    Sperm preparation – dish preparation – sperm selection and isolation of the best-looking spermatozoa – oocyte retrieval – IMSI

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Vanderzwalmen, P. et al. (2019). Intracytoplasmic Morphologically Selected Sperm Injection. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) In Vitro Fertilization. Springer, Cham. https://doi.org/10.1007/978-3-319-43011-9_33

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-43011-9_33

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-43010-2

  • Online ISBN: 978-3-319-43011-9

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation