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Derivation of Dopaminergic Neurons from Human Pluripotent Stem Cells Using a Defined System and/or Small Molecules

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Working with Stem Cells

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Abstract

The ability of pluripotent stem cells (PSC), such as embryonic stem cells and induced pluripotent stem cells, to assume the identity of any other cell type makes them a reliable way to derive cells for disease modeling and cell therapy. Reprogramming patient somatic cells into neurons and other therapeutically relevant cell types offers new strategies for the treatment of neurodegenerative diseases, like Parkinson’s disease. The lineage-specific differentiation of PSCs to produce a large quantity of healthy and/or genetically engineered neurons is the ultimate goal of stem cell therapy for patients with neurodegenerative disorders. Here, we describe two established protocols for the efficient derivation of dopaminergic neurons from PSCs using defined media and/or small molecules. Dopaminergic neurons can be efficiently generated using either of these protocols on a large scale.

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  1. Department of Neurosurgery, School of Medicine, Stanford University, 1050 Arastradero Road, Building A, Room A119, Palo Alto, CA, 94304, USA

    Atossa Shaltouki

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  1. Atossa Shaltouki
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Shaltouki, A. (2016). Derivation of Dopaminergic Neurons from Human Pluripotent Stem Cells Using a Defined System and/or Small Molecules. In: Working with Stem Cells. Springer, Cham. https://doi.org/10.1007/978-3-319-30582-0_12

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