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Thiazole Orange-Tethered Nucleic Acids and ECHO Probes for Fluorometric Detection of Nucleic Acids

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Modified Nucleic Acids

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 31))

Abstract

Thiazole orange (TO) is a well-known fluorescent dye that emits strong fluorescence when it binds to nucleic acid. A number of TO-tethered nucleic acids have been developed for the fluorometric detection of nucleic acids. This chapter summarizes several TO-tethered nucleic acids and shows how the diverse range of such fluorescence-modified nucleic acids enriches the methodology available for DNA sequence typing and RNA monitoring. In addition, the chapter highlights recent advances in exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probes, which have a fluorescence-labeled nucleotide in which two TO subunits are linked covalently. ECHO probes have enabled fluorometric distinction between the probe distribution and target RNA localization in living cells. Further modifications of ECHO probes have made possible a variety of practical applications for intracellular RNA imaging, with the most recent derivatives possessing diverse abilities such as nuclease resistance, photoactivation, self-avoidance, binding to higher-ordered structures, and multicoloring.

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Acknowledgements

The author thanks the members of the Okamoto Laboratory for helpful discussions. The research on ECHO probes in Okamoto Laboratory was supported by the Funding Program for Next Generation World-Leading Researchers, Japan (LR036).

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  1. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan

    Akimitsu Okamoto

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Correspondence to Akimitsu Okamoto .

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  1. The Institute of Scientific and Industri, Osaka University, Ibaraki, Osaka, Japan

    Kazuhiko Nakatani

  2. University of California, San Diego, La Jolla, California, USA

    Yitzhak Tor

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Okamoto, A. (2016). Thiazole Orange-Tethered Nucleic Acids and ECHO Probes for Fluorometric Detection of Nucleic Acids. In: Nakatani, K., Tor, Y. (eds) Modified Nucleic Acids. Nucleic Acids and Molecular Biology, vol 31. Springer, Cham. https://doi.org/10.1007/978-3-319-27111-8_3

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