Skip to main content

Extraction, Purification, and Modification of Natural Polymers

  • Chapter
  • First Online:
Natural Polymers

Abstract

Natural polymers gelatine, chitin, chitosan, cellulose, starch, and pectin are reviewed here with focus on their extraction methods, looking at how they are purified and the extent of modifications in their extraction and utilization. Various techniques exist for the extraction of these polymers from their natural sources which are mainly plants, insects, or fish. Within the chapter the acid and the alkaline processes usually used to extract gelatine are discussed. The gelatin prepared by the acid process is called type A gelatin, whereas that prepared by the alkaline process is called type B gelatine. Sorghum and melon bugs provide an alternative source acceptable for Halal products and serve as an alternative for markets concerned about bovine spongiform encephalopathy (BSE). Chitin extraction involves demineralization and deproteinization steps, which can be conducted by chemical or biological method. The chemical extraction requires the use of acids and bases, while the biological method involves microorganisms. Chitosan is synthesized by deacetylation of chitin using sodium hydroxide to remove the proteins in the source followed by demineralization to remove the carbon and other salts present in crude form. These and others are discussed within the chapter.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Álvarez SPO, Cadavid DAR, Sierra DME, Orozco CPO, Vahos DFR, Ocampo PZ, Atehortúa L (2014). Comparison of extraction methods of chitin from Ganoderma lucidum mushroom obtained in submerged culture. Bio Med Res Int Volume 2014, Article ID 169071, 7 p

    Google Scholar 

  • Aravantinos-Zafiris G, Oreopoulou V (1991) The effect of nitric acid extraction variables on orange pectin. J Sci Food Agri 60:127–129

    Article  Google Scholar 

  • Arbia W, Arbia L, Adour L, Amrane A (2013) Chitin recovery using biological methods. Food Technol Biotechnol 51(1):12–25

    Google Scholar 

  • Arguelles-Monal W, Goycoolea FM, Lizardi J, Peniche C, Higuera-Ciapara I (2003). Chitin and chitosan in gel network systems. In: Bohidar HB, Dubin P, Osada Y, (eds) Polymer gels. ACS Symposium Series No. 833. American Chemical Society, Washington D.C. pp 102–22

    Google Scholar 

  • Belitz HD, Grosch W, Schieberle P (2009) Food chemistry 4th revised and extended ed. Springer, Berlin

    Google Scholar 

  • Brendel O, Iannetta PPM, Stewart D (2000) A rapid and simple method to isolate pure alpha-cellulose. Phytochem Anal 11:7–10

    Article  CAS  Google Scholar 

  • Bryant CM, Hamaker BR (1997) Effect of lime on gelatinization of corn flour and starch. Cereal Chern 74:171–175

    Article  CAS  Google Scholar 

  • Buice RG Jr, Gold TB, Lodder RA, Digenis GA (1995) Determination of moisture in intact gelatin capsules by near-infrared spectroscopy. J Pharm Res 12(1):161–163

    Article  CAS  Google Scholar 

  • Campbell M (2006). Extraction of pectin from watermelon rind. M.Sc. thesis Oklahoma State University, Stillwater. Oklahoma, USA

    Google Scholar 

  • Campus-BaypoJi ON, Rosas-Burgos Ec, Torres-Chavez PI, RamirezWong B, Serna-Saldivar So (1999) Physicochemical changes of starch during maize tortilla production. Starch/Starke 51: 173–177

    Google Scholar 

  • Chatterjee S, Adhya M, Guha AK, Chatterjee BP (2005) Chitosan from Mucor rouxii: production and physico-chemical characterization. Process Biochem 40:395–400

    Article  CAS  Google Scholar 

  • Cho SM, Kwak KS, Park DC, Gu YS, Ji CI, Jang DH, Lee YB, Kim SB (2005) Processing optimization and functional properties of gelatin from shark (Isurus oxyrinchus) cartilage. J Food Hydrocolloids 18(4):573–579

    Article  Google Scholar 

  • Chenite A, Buschmann M, Wang D, Chaput C, Kandani N (2001) Rheological characterization of thermogelling chitosan/glycerol-phosphate solutions. Carbohydr Polym 46:39–47

    Article  CAS  Google Scholar 

  • Cole CGB, Roberts eJJ (1996) Changes in the molecular composition of gelatin due to the manufacturing process and animal age, as shown by electrophoresis. J Soc Leather Technol Chem, 80(5): 136–141

    Google Scholar 

  • Cole CGB (2000) Gelatin. Francis FJ (ed) Encyclopedia of food science and technology, 2nd edn. John Wiley & Sons, New York. 4 vols, pp 1183–1188

    Google Scholar 

  • Einbu A, Naess SN, Elgsaeter A, Varum KM (2004) Solution properties of chitin in alkali. Biomacromolecules 5:2048–2054

    Article  CAS  Google Scholar 

  • Fernandez-Diaz MD, Montero P, Gomez-Guillen MC (2001) Gel properties of collagens from skins of cod (Gadus morhua) and hake (Merluccius merluccius) and their modification by the co-enhancers magnesium sulphate, glycerol and transglutaminase. Food Chem 74:161–167

    Article  CAS  Google Scholar 

  • Gao W, Lai JCK, Leung SW (2012). Functional enhancement of chitosan and nanoparticles in cell culture, tissue engineering, and pharmaceutical applications. Front Physiol 3:321

    Google Scholar 

  • Gomez MH, Waniska RD, Rooney LW (1991) Starch characterization of nixtamalized com flour. Cereal Chern 68:578–582

    CAS  Google Scholar 

  • Gomez MH, Lee JK, McDonough CM, Waniska RD, Rooney LW (1992) Com starch changes during tortilla and tortilla chip processing. Cereal Chern 69:275–279

    CAS  Google Scholar 

  • Gomez MH, Rooney Lw, Waniska RD, and Pflugfelder RL (1987) Dry com masa flours for tortilla and snack processing. Cereal Foods World 32:372–377

    Google Scholar 

  • Gaudinski JB, Dawson TE, Quideau S, Schuur EAG, Roden JS, Trumbore SE, Sandquist DR, Oh S-W, Wasylishen RE (2005) Comparative analysis of cellulose preparation techniques for use with 13C, 14C and 18O isotopic measurements. Anal Chem 77(22):7212–7224

    Article  CAS  Google Scholar 

  • Gimenez B, Turnay J, Lizarbe MA, Montero P, Gomez-Guillen MC (2005) Use of lactic acid for extraction of fish skin gelatin. J Food Hydrocolloids 19:941–950

    Article  CAS  Google Scholar 

  • Gómez-Guillén MC, Montero P (2001) Extraction of gelatin from megrim (Lepidorhombus boscii) skins with several organic acids. J Food Sci 66(213–6):26

    Google Scholar 

  • Gomez-Guillen MC, Turnay J, Fernandez-Diaz MD, Ulmo N, Lizarbe MA, Montero P (2002) Structural and physical properties of gelatin extracted from different marine species: a comparative study. J Food Hydrocolloids 16:25–34

    Article  CAS  Google Scholar 

  • Gudmundsson M (2002a) Rheological properties of fish gelatins. J Food Sci 67:2172–2175

    Article  CAS  Google Scholar 

  • Gudmundsson M (2002b) Rheological properties of fish gelatins. J Food Sci 67:2172–2175

    Article  CAS  Google Scholar 

  • Gudmundsson M, Hafsteinsson H (1997) Gelatin from cod skins as affected by chemical treatments. J Food Sci 62(1):3937–3947

    Article  Google Scholar 

  • Han X-Z, Benmoussa M, Gray JA, BeMiller JN, Hamaker BR (2005) Detection of proteins in starch granule channels. Cereal Chern. 82:351–355

    Article  CAS  Google Scholar 

  • Hao S, Li L, Yang X, Cen J, Shi H, Bo Q et al. (2009) The characteristics of gelatin extracted from sturgeon (Acipenser baeri) skin using various pretreatments. Food Chem, 115:124e128

    Google Scholar 

  • IPPA: International Pectin Producers Association (2001) What is pectin? Available online at URL http://www.ippa.info/commercial production of pectin.htm. Accessed Mar 2015

  • Jamilah B, Harvinder KG (2002) Properties of gelatins from skins of fish—black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food Chem 77:81–84

    Article  CAS  Google Scholar 

  • Joesten MD, Wood JL (1996). The World of Chemistry, 2nd edn. Fort Worth, TX: Saunders College. Read more: http://www.chemistryexplained.com/Pl-Pr/Polymers-Natural.html#ixzz3WV1myXMB

  • Jusila J (2004) Preparing ballistic gelatin—review and proposal for standard method. J Forensic Sci Int 14:91–98

    Article  Google Scholar 

  • Kilpelainen I, Xie H, King A, Granstrom M, Heikkinen S, Argyropoulos DS (2007) J Agric Food Chem 55:9142–9148

    Article  Google Scholar 

  • Khule R, Mahale NB, Shelar DS, Rokade MM, Chaudhari SR (2012) Extraction of pectin from citrus fruit peel and use as natural binder in paracetamol tablet Nilesh. Der Pharmacia Lettre 4(2):558–564

    CAS  Google Scholar 

  • Kubota N, Eguchi Y (1997) Facile preparation of water-soluble N-acetylated chitosan and molecular weight dependence of its water-solubility. Polym J 29:123–127

    Article  CAS  Google Scholar 

  • Leavitt SW, Danzer SR (1993) Method for processing small wood samples to holocellulose for stable-carbon isotope analysis. Anal Chem 65(1):87–90

    Article  CAS  Google Scholar 

  • Lim S-T, Lee J-H, Dong-Hoon SK, Lim HS (1999) Comparison of protein extraction solutions for rice starch isolation and effects of residual protein content on starch pasting properties. Starch/Stärke 51(4):120–125

    Article  CAS  Google Scholar 

  • Liu Y, Liu X, Wang X (2011) Biomimetic synthesis of gelatin polypeptideassisted noble-metal nanoparticles and their interaction study. Nanoscale Res Lett 6:22

    Google Scholar 

  • Maningat CC, Juliano BO (1979). Properties of lintnerized starch granules from rices differing in amylose content and gelatinization temperature. Starch/Stärke 31:5–10

    Google Scholar 

  • Mariod AA, Abdel-Wahab SI, Ain NM (2011a) Proximate amino acid, fatty acid and mineral composition of two Sudanese edible pentatomid insects. Int J Trop Insect Sci 31(3):145–153

    Article  Google Scholar 

  • Mariod AA, Abdelwahab SI, Ibrahim MY, Mohan S, Abd Elgadir M, Ain NM (2011b) Preparation and characterization of gelatins from two sudanese edible insects. J Food Sci Eng 1:45–55

    CAS  Google Scholar 

  • Montero P, Gomez-Guillen MC (2000) Extracting conditions for megrim (Lepidorhombus boscii) skin collagen affect functional properties of the resultant gelatin. Food Sci 65:434–438

    Article  CAS  Google Scholar 

  • Monteil-Rivera F, Huang GH, Paquet L, Deschamps S, Beaulieu C, Hawari J (2012) Microwave-assisted extraction of lignin from triticale straw: optimization and microwave effects. Bioresour Technol 104:775–782

    Article  CAS  Google Scholar 

  • Nishimoto M, Sakamoto R, Mizuta S, Yoshinaka R (2005) Identification and characterization of molecular species of collagen in ordinary muscle and skin of the Japanese flounder Paralichthys olivaceus. Food Chem 90:151–156

    Article  CAS  Google Scholar 

  • Norland RE (1990) Fish gelatin. In: Voight MN, Botta JK (eds) Advances in fisheries technology and biotechnology for increased profitability. Technomic Publishing Co., Lancaster, Pa., pp 325–333

    Google Scholar 

  • Paul S, Jayan A, Sasikumar CS, Cherian SM (2014) Extraction and purification of chitosan from chitin isolated from sea prawn Fenneropenaeus indicus. Asian J Pharm Clin Res 7(4):201–204

    Google Scholar 

  • Pałasiński M (2005) Technology of carbohydrate processing (in Polish). Polish Society of Food Technologists, Małopolska Branch, Kraków, pp 63–76

    Google Scholar 

  • Puvvada YS, Vankayalapati S, Sukhavasi S (2012) Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. Int Curr Pharm J 1(9):258–263

    Article  CAS  Google Scholar 

  • Pu Y, Jiang N, Ragauskas AJ (2007) J Wood Chem Technol 27:23–33

    Article  CAS  Google Scholar 

  • Pye EK, Lora JH (1991) The Al cell process. A proven alternative to kraft pulping. Tappi J 74:113–118

    CAS  Google Scholar 

  • Phatak L, Chang KC, Brown G (1988) Isolation and characterization of pectin in sugarbeet pulp. J Food Sci 53(3):830–833

    Article  CAS  Google Scholar 

  • Pinkert A, Goeke DF, Marsh KN, Pang S (2011) Extracting wood lignin without dissolving or degrading cellulose: investigations on the use of food additive-derived ionic liquids. Green Chem 13:3124

    Article  CAS  Google Scholar 

  • Ratnayake WS, Wassinger AB, Jackson DS (2007) Extraction and characterization of starch from alkaline cooked corn masa. Cereal Chern 84(4):415–422)

    Google Scholar 

  • Rinaudo M (2006) Chitin and chitosan: properties and applications. Prog Polym Sci 31(2006):603–632

    Article  CAS  Google Scholar 

  • Samal SK, Dash M, Van Vlierberghe S, Kaplan DL, Chiellini E, Van Blitterswijk C, Moronid L, Dubruel P (2012) Cationic polymers and their therapeutic potential. Chem. Soc. 41:7147–7194

    Google Scholar 

  • Schrieber R, Gareis H (2007) From Collagen to Gelatine, in Gelatine Handbook: Theory and Industrial Practice. Wiley-VCH Verlag GmbH & Co, KGaA, Weinheim, Germany

    Book  Google Scholar 

  • Shahidi F, Xiao-Qing H, Synowiecki J (1995) Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem 53:285–293

    Article  CAS  Google Scholar 

  • Stainsby G (1987) Gelatin gels. In: Pearson AM, Dutson TR, Bailey AJ (eds) Advances in meat research, vol 4 Collagen as a food. Van Nostrand Reinhold Co. Inc., New York, pp 209–222. Ms. 20000125

    Google Scholar 

  • Serna-Saldivar SO, Gomez MH, Rooney LW (1990) Technology, chemistry, and nutritional value of alkaline-cooked com products, pp 243–307. In: Pomeranz Y (ed) Advances in cereal science and technology, vol X. AACC International, St. Paul, MN

    Google Scholar 

  • Shahidi Fereidoon, Synowiecki Jozef (1991) Isolation and characterization of nutrients and value-added products from snow crab (Chionoecetes opilio) and shrimp (Pandalus borealis) processing discards. J Agric Food Chem 39(8):1527–1532

    Article  CAS  Google Scholar 

  • Song C, Yu H, Zhang M, Yang Y, Zhang G (2013) Physicochemical properties and antioxidant activity of chitosan from the blowfly Chrysomya megacephala larvae. Int J Biol Macromol 60:347–354

    Article  CAS  Google Scholar 

  • Thomas K, Susanne R, Maya T (2005) “Trimethylated chitosans as non-viral gene delivery vectors: cytotoxicity and transfection efficiency”. J Control Release 103(3):643–653)

    Google Scholar 

  • Wang X, Uchiyama S (2013). Polymers for biosensors constructions. State of the art in biosensors-general aspects, pp 68–86

    Google Scholar 

  • Wu Y, Lin QL, Chen ZX, Wu W, Xiao HX (2011). Preparation of chitosan oligomers COS and their effect on the retrogradation of intermediate amylose rice starch. J Food Sci Technol. doi:10.1007/s13197-010-0210-2

    Google Scholar 

  • Xia Z, Wu Sh, Chen J (2013) Preparation of water soluble chitosan by hydrolysis using hydrogen peroxide. Int J Biol Macromol 59(2013):242–245

    Article  CAS  Google Scholar 

  • Zapata PA, Rojas DF, Atehortúa L (2012) Production of biomass, polysaccharides, and ganoderic acid using non-conventional carbon sources under submerged culture of the lingzhi or reishi medicinal mushroom, Ganoderma lucidum (W.Curt.:Fr.) P. Karst., (higher basidiomycetes). Int J Med Mushrooms 12(2):197–203

    Google Scholar 

  • Zhou P, Regenstein JM (2004) Optimization of extraction conditions for Pollock skin gelatin. J Food Sci 69:393–398

    Google Scholar 

  • Zoia L, King TAW, Argyropoulos DS (2011). Molecular weight distributions and linkages in lignocellulosic materials derivatized from ionic liquid media. J Agric Food Chem 59:829–838

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Abdalbasit Adam Mariod .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Mariod, A.A. (2016). Extraction, Purification, and Modification of Natural Polymers. In: Olatunji, O. (eds) Natural Polymers. Springer, Cham. https://doi.org/10.1007/978-3-319-26414-1_3

Download citation

Publish with us

Policies and ethics