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The Hope for iPSC in Lung Stem Cell Therapy and Disease Modeling

  • Chapter
Lung Stem Cells in the Epithelium and Vasculature

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

The discovery of induced pluripotent stem cell technology has provided revolutionary access to human biology. Studying human lung disease is notoriously difficult; iPSC provide the potential for the generation of reproducible systems for investigating the cellular, molecular, and genetic mechanisms that lead to the development and pathogenesis of human lung disease. In the future, this technology holds great potential for the development of novel screening techniques and even cell-based therapeutic approaches for human lung disease. This chapter discusses how iPSC and gene editing technologies are being applied to study human lung disease.

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Abbreviations

3D:

Three dimensional

AAV:

Adeno-associated virus

AD:

Alzheimer’s disease

AFE:

Anterior foregut endoderm

ALI:

Air–liquid interface

ATI/II:

Alveolar type I /II cells

BMP4:

Bone morphogenic protein 4

Bromo cAMP:

Bromo cyclin adenosine monophosphate

CC10:

Club cell 10 kDa protein

CD54:

Cluster of differentiation factor 54 (aka: ICAM-1 (intercellular adhesion molecule 1))

CDX2:

Caudal type homeobox 2

CFTR:

Cystic fibrosis transmembrane regulator

ChIP:

Chromatin immuno precipitation

CK5:

Cytokeratin 5

COPD:

Chronic obstructive pulmonary disease

CRISPR:

Clustered regularly interspersed short palindromic repeats

DE:

Definitive endoderm

DSB:

Double-stranded break

EGF:

Epidermal growth factor

ESCs:

Embryonic stem cells

FD:

Familial dysautonomia

FGF10:

Fibroblast growth factor 10

FOXA2:

Forkhead box A2

FOXJ1:

Forkhead box J1

gRNA:

Guide ribonucleic acid

HR:

Homologous recombination

iPSC:

Induced pluripotent stem cells

KGF:

Keratinocyte growth factor (aka: FGF7)

KLF4:

Kruppel-like factor 4

MAPK/ERK:

Mitogen-activated protein kinase/extracellular signal-regulated kinase

MCC:

Multiciliated cell

MUC5AC:

Mucin 5 AC

NHEJ:

Non-homologous end joining

Nkx2.1:

NK2 homeobox 1 (aka: TTF1 thyroid transcription factor 1)

OCT4:

Octamer-binding transcription factor 4 (aka: POU5F1 (POU domain class 5, transcription factor 1))

PCD:

Primary ciliary dyskinesia

RA:

Retinoic acid

SCZD:

Schizophrenia

SHH:

Sonic hedgehog

SMA:

Spinal muscular atrophy

SOX2:

Sex-determining region Y box2

T-1α:

aka: Podoplanin

TALEN:

Transcription activator-like effector nuclease

TGFβ:

Transforming growth factor beta

Wnt3a:

Wingless-type MMTV integration site family member 3A

ZFNs:

Zinc finger nucleases

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Authors and Affiliations

  1. Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Tushar Menon & Amy L. Firth Ph.D.

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  1. Tushar Menon
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  2. Amy L. Firth Ph.D.
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Correspondence to Amy L. Firth Ph.D. .

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Editors and Affiliations

  1. Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, California, USA

    Amy Firth

  2. Department of Medicine, The Univsersity of Arizona, Tucson, Arizona, USA

    Jason X.-J. Yuan

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Menon, T., Firth, A.L. (2015). The Hope for iPSC in Lung Stem Cell Therapy and Disease Modeling. In: Firth, A., Yuan, JJ. (eds) Lung Stem Cells in the Epithelium and Vasculature. Stem Cell Biology and Regenerative Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-16232-4_7

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