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Some Techniques to Elaborate Plant–Microbe Interactions

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Beneficial Plant-Bacterial Interactions

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Abstract

With the advent of recombinant DNA technology in the early 1970s and the subsequent development of a host of other technologies including DNA sequencing, the polymerase chain reaction (PCR), and DNA synthesis, it is possible to isolate, characterize, amplify, and sequence virtually any gene of interest. Moreover, the more recently developed techniques of transcriptomics, proteomics, and metabolomics may be employed to understand in considerable detail changes in plants that occur as a consequence of an interaction with PGPB as well as changes in PGPB that result from interactions with plants. The use of these newer techniques is enabling researchers to develop a more profound understanding of the detailed nature of plant–bacterial interactions, an important step in the development of new and more efficacious PGPB strains. Here, as a starting point, a number of those techniques are examined and discussed.

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  1. Department of Biology, University of Waterloo, Waterloo, ON, Canada

    Bernard R. Glick

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Correspondence to Bernard R. Glick .

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Glick, B.R. (2015). Some Techniques to Elaborate Plant–Microbe Interactions. In: Beneficial Plant-Bacterial Interactions. Springer, Cham. https://doi.org/10.1007/978-3-319-13921-0_4

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