The Research of Ascorbic Acid-Loaded Gelatin Nanoparticles and Cell Uptake Studies with NCTC Clone 929 Cell Line

Abstract

One of the reason caused the production of scar is the excessive sediment or the overtime precipitation during the wounds healing. So far amount all the medical supplies, hydrocolloid dressings which used to repair the burn wounds and the normal trauma are bass on promotion of wound healing. Nevertheless there are no medical supplies combine hydrocolloid dressings with anti-pigmentation drugs; therefor our research has focused on preparing gelatin nanoparticle. First, prepared different size of particles by controlling the parameters. Second, loaded various concentrations of ascorbic acid into particles, analyzed the drug entrapment efficiency and the rate of drug release. Last, co-culture nanoparticle with fibroblast, research the effect of nanoparticle on the fibroblast, in order to prepare a successful anti pigmentation gelatin nanoparticle. Under distinct condition, particle size 150nm ~ 300nm of gelatin nanoparticle could be prepared, furthermore the effective concentration of ascorbic acid 0.3%~10% is successfully completed and the drug-loaded nanoparticles are able to extend the life-span of the fibroblast.