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Recombination and Gene Targeting in Neurospora

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Genetic Transformation Systems in Fungi, Volume 1

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Genetic manipulation, especially targeted gene replacement, is a potential powerful tool for gene functional research and industrial engineering in filamentous fungi. However, low frequency of gene targeting in most filamentous fungi has hampered research on the molecular mechanisms of these species. In this chapter, we describe the relationship between exogenous DNA integration events and cellular DNA double-strand repair machinery in one of the model filamentous fungi, Neurospora crassa. Based on the molecular mechanism, it has been proven that the gene-targeting frequency is dramatically increased when nonhomologous end-joining, that promotes chromosomal random integration, was deficient in Neurospora and various other fungi. This technique has opened a new avenue for genetic manipulation in filamentous fungi.

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Authors and Affiliations

  1. Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University, Saitama, Japan

    Keiichiro Suzuki Ph.D.

  2. Regulation Biology, Faculty of Science, Saitama University, Saitama, Japan

    Hirokazu Inoue Ph.D.

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  1. Keiichiro Suzuki Ph.D.
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  2. Hirokazu Inoue Ph.D.
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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Suzuki, K., Inoue, H. (2015). Recombination and Gene Targeting in Neurospora . In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_24

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