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Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle

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Genetic Transformation Systems in Fungi, Volume 1

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Abstract

Capability of taking up exogenous DNA of yeast Saccharomyces cerevisiae cells was studied by lipophilic cations accumulation and FT-IR spectroscopy. The increase in permeability of the yeast cells treated with Li+ ions was observed. The changes in the cell wall structure detected by FT-IR spectra were associated with transformability of yeast cells. The presence of a population of cells with buds with a higher permeability to TPP cations was determined. Relationship between transformation efficiency of yeast Saccharomyces cerevisiae and cell cycle was determined. Yeast cells in the S-phase of cell cycle showed an enhanced perception to exogenous DNA. An increase was associated with physiological and permeability properties of yeast cells. The phenomenon of natural competence is supposed to occur in yeast cells.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Vilnius University, Vilnius, Lithuania

    Aurelijus Zimkus Ph.D.

  2. Center for Physical Sciences and Technology, Vilnius, Lithuania

    Aurelijus Zimkus Ph.D. & Arūnas Ramanavičius Ph.D.

  3. Department of Organic Chemistry, Center for Physical Sciences and Technology, Vilnius, Lithuania

    Audrius Misiūnas Ph.D.

  4. Department of Biopharmaceutical, Centre of Innovative Medicine, Vilnius, Lithuania

    Audrius Misiūnas Ph.D.

  5. Center of Nanotechnology and Materials Science—NanoTechnas, Faculty of Chemistry, Vilnius University, Vilnius, Lithuania

    Arūnas Ramanavičius Ph.D.

  6. Department of Bioelectrochemistry and Biospectroscopy Institute of Biochemistry, Vilnius University, Vilnius, Lithuania

    Larisa Chaustova Ph.D.

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Correspondence to Aurelijus Zimkus Ph.D. .

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  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Zimkus, A., Misiūnas, A., Ramanavičius, A., Chaustova, L. (2015). Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_23

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