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Polymeric Cryogels pp 245–281Cite as

Cryogels for Biotechnological Applications

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Part of the book series: Advances in Polymer Science ((POLYMER,volume 263))

Abstract

Cryogels are formed in a semifrozen state when the solvent is frozen, but solutes are still soluble. The ice crystals are porogens and, upon thawing the system, pores appear where the frozen solvent was found earlier. Such gels have large pores, are elastic, and offer interesting opportunities in biotechnology. Cryogels with their large pores can meet demands that traditional chromatographic media cannot. This also opens up opportunities for the separation of cells because upon passage through the gel cells may interact with specific groups on the pore walls, thereby becoming retarded and/or captured. A range of applications have been studied: isolation of microbial cells, capturing of cancer cells, and use of cryogels as matrices for immobilized cell reactors. Furthermore, the robustness of the gels allows new applications, for example in environmental separation.

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Abbreviations

AAm:

Acrylamide

BSA:

Bovine serum albumin

ECS:

Extra capillary space

HFR:

Hollow fiber reactor

HMs:

Heavy metal ions

HSA:

Human serum albumin

ICS:

Intra capillary space

IDA:

Immuno-diacetic-acid

MIP:

Molecularly imprinted polymer

MPG:

Macroporous gel particle

NIP:

Non-imprinted polymer

NIPA:

N-Isopropyl acrylamide

PAAm:

Polyacrylamide

PEG:

Polyethylene glycol

PHEMA:

Poly(2-hydroxyethyl methacrylate)

PVA:

Polyvinyl alcohol

SEM:

Scanning electron microscope

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Acknowledgements

This work was supported by The Swedish Research Council.

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Authors and Affiliations

  1. Department of Biotechnology, Lund University, Lund, Sweden

    Bo Mattiasson

  2. Indienz AB, c/o Department of Chemistry, Lund University, Lund, Sweden

    Bo Mattiasson

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  1. Bo Mattiasson
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Correspondence to Bo Mattiasson .

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  1. Department of Chemistry, Istanbul Technical University, Istanbul, Turkey

    Oguz Okay

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Mattiasson, B. (2014). Cryogels for Biotechnological Applications. In: Okay, O. (eds) Polymeric Cryogels. Advances in Polymer Science, vol 263. Springer, Cham. https://doi.org/10.1007/978-3-319-05846-7_7

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