Summary
Escherichia coli transformed by a composite plasmid-containing α-amino-ε-caprolactam racemase structural gene, downstream to the tac promoter, showed a high productivity, which made the enzyme purification and crystallization easier. Complete nucleotide sequence was determined by collaboration of shotgun DNA-sequencing and automatic protein sequencing of lysylendopeptidase digest, which revealed that the racemase consists of 435 amino acids.
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© 1987 Birkhäuser Verlag Basel
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Naoko, N., Oshihara, W., Yanai, A. (1987). α-Amino-ε-Caprolactam Racemase for L-Lysine Production. In: Korpela, T.K., Christen, P. (eds) Biochemistry of Vitamin B6 . Birkhäuser Congress Reports. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-9308-4_81
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DOI: https://doi.org/10.1007/978-3-0348-9308-4_81
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-9989-5
Online ISBN: 978-3-0348-9308-4
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