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Free radical scavenger properties of EGB 761 on functional disorders induced by experimental diabetic retinopathy

  • Conference paper
Oxidative Stress and Aging

Part of the book series: Molecular and Cell Biology Updates ((MCBU))

Summary

Several experiments clearly show a release of oxygenated free radicals in diabetic retinas, linked to a marked increase of the lipoperoxidation. To protect retina against diabetic disorders, we tested Ginkgo biloba extract (EGb 761, IPSEN, France) a potent free radical scavenger (O −2 , OH·). On two studies, we measured EGb 761 protective effects (i) on electroretinogram (ERG) and (ii) ion shifts disturbances (Na2+, Ca2+ and K+) after ischemia-reperfusion. We experimented on male albino Sprague-Dawley rats. Diabetes was induced by injection of a single dose of streptozotocin. In a first experiment, we tested EGb 761 on retinal functional disorders by recording ERG. EGb 761 was orally given in daily administration beginning 15 days before streptozotocin administration at the dose of 100 mg/kg. In the second experiment, on diabetic rats, we induced ischemiareperfusion, by clamping the retinal artery for 90 min, followed by reperfusion for 24 h. We tested EGb 761 orally, given at different doses (25, 50 and 100 mg/kg) in daily administration beginning 10 days before ischemiareperfusion. We measured ion shifts by atomic absorption spectrophotometry. Our results indicated that (i) experimental diabetes recorded after 12 or 14 weeks was responsible for a marked impairment of ERG profile: b wave was delayed and asymmetric. These ERG typical modifications were totally absent after EGb 761 treatment. (ii) Ischemia-reperfusion on diabetic rats resulted in ionic imbalance more important than in normal rats. We noted Na+, Ca2+ tissue accumulation and K+ loss. According to a dose-response effect, EGb 761 significantly attenuated these ion disturbances. In conclusion, it appears that oxygenated free radicals may be implicated in retinal disorders due to diabetes. A free radical scavenger, such as EGb 761, may contribute to the prevention of the ERG disturbances and to the recovery of the retinal function even after ischemia-reperfusion.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, IPSEN Institute, 24 rue Erlanger, F-75016, Paris, France

    M.-T. Droy-Lefaix

  2. Department of Surgery, The School of Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut, 06030-1110, USA

    E. Szabo-Tosaki

  3. INSERM U. 71, Biophysics Laboratory, 28 Place Henri Dunant, F-63001, Clermont-Ferrand, France

    M. Doly

Authors
  1. M.-T. Droy-Lefaix
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  2. E. Szabo-Tosaki
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  3. M. Doly
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Editor information

Editors and Affiliations

  1. Gerontology Research Center, National Institute on Aging, NIH, 21224, Baltimore, MD, USA

    R. G. Cutler

  2. Dept. Molecular and Cell Biology, 251 Life Science Addition, Membrane Bioenergetics Group, 94720, Berkeley, CA, USA

    L. Packer

  3. Cancer Research Center of Hawaii, Molecular Oncology, University of Hawaii at Manoa, 1236 Lauhala Street, 96813, Honolulu, HI, USA

    J. Bertram

  4. Dept. of Neuroscience, Institute of Cellular and Molecular Medicine, Okayama University, 2-5-1 Shikatacho, Okayama 700, Japan

    A. Mori

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© 1995 Birkhäuser Verlag Basel/Switzerland

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Droy-Lefaix, MT., Szabo-Tosaki, E., Doly, M. (1995). Free radical scavenger properties of EGB 761 on functional disorders induced by experimental diabetic retinopathy. In: Cutler, R.G., Packer, L., Bertram, J., Mori, A. (eds) Oxidative Stress and Aging. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7337-6_26

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  • DOI: https://doi.org/10.1007/978-3-0348-7337-6_26

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7339-0

  • Online ISBN: 978-3-0348-7337-6

  • eBook Packages: Springer Book Archive

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