Abstract
Tissue engineering is intended for the reconstruction of lost tissue due to destructive diseases such as inflammation and tumors, and requires three major elements: cells, signalling molecules and scaffolds. A scaffold is an extracellular artificial matrix with three dimensions; it is highly porous, 3D printed, and able to host and guide cells in their growth and in tissue regeneration. Currently, collagen hydrogels are the most popular material for cellular scaffolds, used successfully in clinical practice. Graphene oxide, graphene´s precursor in its synthesis, is a graphite oxide monolayer; even though the study of its properties for tissue engineering is still in its early stages, big results are expected. The project aims at designing, 3D printing and evaluating the features of collagen and graphene oxide scaffolds, for potential applications in tissue engineering. The ink obtained from these materials allowed loyal printings, respecting structures and dimensions. The incorporation of graphene oxide in collagen scaffolds showed variations in their properties, keeping swelling ratio, decreasing porosity and solubility. Using a scanning electron microscope (SEM), we distinguished pure graphene oxide from collagen in the scaffold, where we observed the combination of both materials in micro-scale.
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Acknowledgements
The authors acknowledge the contribution of IIByT (CONICET-UNC), Departamento de Química Orgánica y Biológica (FCEFyN-UNC), Departamento de Química (FCEFQyN-UNRC), Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (FCQ-UNC), and LAMARX (FAMAF-UNC) to carry out this project.
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Cáceres, M.V., Fernández, P.A., Morales, G., Salvatierra, N.A., Comín, R., Cid, M.P. (2024). Design and Evaluation of Graphene Oxide/Collagen Scaffolds for Application in Tissue Engineering. In: Lopez, N.M., Tello, E. (eds) Advances in Bioengineering and Clinical Engineering. SABI 2022. IFMBE Proceedings, vol 105. Springer, Cham. https://doi.org/10.1007/978-3-031-51723-5_5
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