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Comprehensive Spatial Lipidomics of Formalin-Fixed Paraffin-Embedded Tissue Guided by Mass Spectrometry-Imaging

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A Practical Guide to Metabolomics Applications in Health and Disease

Part of the book series: Learning Materials in Biosciences ((LMB))

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Abstract

This chapter aims to provide a practical guide to performing comprehensive spatial lipidomics of formalin-fixed paraffin-embedded (FFPE) tissue guided by Matrix Assisted Laser Desorption/Ionisation-mass spectrometry-imaging (MALDI-MSI), presenting an overview of the key methodological aspects as well as the type of data that can be obtained when using this approach. Moreover, it also aims to highlight the more extensive and reliable lipid identifications that can be obtained when an additional trapped ion mobility spectrometry (TIMS) dimension is employed.

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Acknowledgements

The work presented in this chapter was facilitated by Fondazione Gigi & Pupa Ferrari Onlus and Regione Lombardia: programma degli interventi per la ripresa economica: sviluppo di nuovi accordi di collaborazione con le università per la ricerca, l’innovazione e il trasferimento tecnologico.

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Authors and Affiliations

  1. University of Milano-Bicocca, School of Medicine and Surgery, Vedano al Lambro, Italy

    Vanna Denti, Martin Piazza, Andrew Smith & Giuseppe Paglia

Authors
  1. Vanna Denti
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  2. Martin Piazza
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  4. Giuseppe Paglia
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Correspondence to Giuseppe Paglia .

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Editors and Affiliations

  1. Faculty of Biology & Medicine, University of Lausanne, Lausanne, Switzerland

    Julijana Ivanisevic

  2. Center for Proteomics & Metabolomics, Leiden University Medical Center, Leiden, The Netherlands

    Martin Giera

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Denti, V., Piazza, M., Smith, A., Paglia, G. (2023). Comprehensive Spatial Lipidomics of Formalin-Fixed Paraffin-Embedded Tissue Guided by Mass Spectrometry-Imaging. In: Ivanisevic, J., Giera, M. (eds) A Practical Guide to Metabolomics Applications in Health and Disease. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-031-44256-8_14

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  • DOI: https://doi.org/10.1007/978-3-031-44256-8_14

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