Abstract
The increasing resistance of pathogens to a number of antibiotics has been the subject of many research reports from the European Antibiotic Resistance Surveillance Network (Ears-Net) and The World Health Organization (The WHO). The aim of this work was to study the effects of nanomaterial dispersions as Selenium (Se), Gold (Au), Iron oxide (Fe2O3), Silicon dioxide (SiO2) and Graphene oxide (GO) on bacteria like Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus and two strains of Escherichia coli. Two classical methods were used to investigate the antibacterial effect of the nanoparticles (NPs): Spot and Well diffusion tests in agar medium. The tested nanoparticles were active against Gram-positive bacteria in concentrations between 3.0 and 1.5 mg/mL but they were not active against Gram-negative bacteria such as E. coli. Among tested nanomaterials, SeNPs express the strongest antimicrobial effect. Gold nanoparticles with Polyvinylpyrolidone (Au-PVP NPs) were more active against bacteria than pure AuNPs. Lower concentrations (1.0 mg/mL and 0.5 mg/mL) of Se, GO and the two types of Gold nanoparticles did not show activity against all test microorganisms. Fe2O3 NPs as well as SiO2 NPs had no effect on any test bacteria in the mentioned concentrations.
In conclusion, the most cytotoxic for tested bacteria were SeNPs, followed by Au-PVP and AuNPs. GONPs also showed a certain cytotoxic effect, especially on B. cereus 1095.
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References
European Antimicrobial Resistance Surveillance Network (EARS-Net). https://www.ecdc.europa.eu/en/about-us/partnerships-and-networks/disease-and-laboratory-networks/ears-net
WHO Report (2014). http://www.who.int/drugresistance/documents/surveillancereport/en
Cabral, R.M., Baptista, P.V.: The chemistry and biology of gold nanoparticle-mediated photothermal therapy: promises and challenges. Nano LIFE 03(03), 1330001 (2013)
Thambirajoo, M., et al.: Potential of nanoparticles integrated with antibacterial properties in preventing biofilm and antibiotic resistance. Antibiotics 10(11), 1338 (2021)
Ivanova, I., Tsacheva, I.: Microbial sensors based on nanostructures. Recent Pat. Nanomed. 5(1), 59–65 (2015)
Aasi, A., et al.: A density functional theory study on the interaction of toluene with transition metal decorated carbon nanotubes: a promising platform for early detection of lung cancer from human breath. Nanotechnology 31(41), 415707 (2020)
Lu, A.-H., et al.: Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew. Chem. Int. Ed. 46(8), 1222–1244 (2007)
Pelgrift, R.Y., Friedman, A.J.: Nanotechnology as a therapeutic tool to combat microbial resistance. Adv. Drug Deliv. Rev. 65(13–14), 1803–1815 (2013)
Gentile, F., et al.: DNA damage by lipid peroxidation products: implications in cancer, inflammation and autoimmunity. AIMS Genet. 04(02), 103–137 (2017)
Tang, L., Cheng, J.: Nonporous silica nanoparticles for nanomedicine application. Nano Today 8(3), 290–312 (2013)
Gangwar, R.K., et al.: Curcumin conjugated silica nanoparticles for improving bioavailability and its anticancer applications. J. Agric. Food Chem. 61(40), 9632–9637 (2013)
Jeelani, P.G., Mulay, P., Venkat, R., Ramalingam, C.: Multifaceted application of silica nanoparticles. A review. SILICON 12(6), 1337–1354 (2019). https://doi.org/10.1007/s12633-019-00229-y
Feicht, P., Eigler, S.: Defects in graphene oxide as structural motifs. ChemNanoMat 4(3), 244–252 (2018)
Gutiérrez-Portocarrero, S., et al.: Study of structural defects on reduced graphite oxide generated by different reductants. Diam. Relat. Mater. 92, 219–227 (2019)
Sun, L., et al.: A force-engineered lint roller for superclean graphene. Adv. Mater. 31, 1902978 (2019)
Liu, Y., et al.: Investigation on fluorescence quenching of dyes by graphite oxide and graphene. Appl. Surf. Sci. 257(13), 5513–5518 (2011)
Liu, Y., et al.: Biocompatible graphene oxide-based glucose biosensors. Langmuir 26(9), 6158–6160 (2010)
Gupta, D.K., Rajaura, R.S., Sharma, K.: Synthesis and characterization of graphene oxide nanoparticles and their antibacterial activity. Int. J. Environ. Sci. Technol. 1(1), 16–24 (2015)
Verissimo, T.V., et al.: In vitro cytotoxicity and phototoxicity of surface-modified gold nanoparticles associated with neutral red as a potential drug delivery system in phototherapy. Mater. Sci. Eng. C 65, 199–204 (2016)
Shakibaie, M., et al.: Biosynthesis and recovery of selenium nanoparticles and the effects on matrix metalloproteinase-2 expression. Biotechnol. Appl. Biochem. 56(1), 7–15 (2010)
Wang, H., et al.: Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radic. Biol. Med. 42(10), 1524–1533 (2007)
Iranifam, M., et al.: A novel selenium nanoparticles-enhanced chemiluminescence system for determination of dinitrobutylphenol. Talanta 107, 263–269 (2013)
Wang, Q., Webster, T.J.: Nanostructured selenium for preventing biofilm formation on polycarbonate medical devices. J. Biomed. Mater. Res. A 100(12), 3205–3210 (2012)
Tran, P.A., Webster, T.J.: Antimicrobial selenium nanoparticle coatings on polymeric medical devices. Nanotechnology 24(15), 155101 (2013)
Boroumand, S., et al.: Selenium nanoparticles: synthesis, characterization and study of their cytotoxicity, antioxidant and antibacterial activity. Mater. Res. Express 6(8), 0850d8 (2019)
Choi, O., et al.: The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Res. 42(12), 3066–3074 (2008)
Vargas-Reus, M.A., et al.: Antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens. Int. J. Antimicrob. Agents 40(2), 135–139 (2012)
Suchak, N.M., et al.: Study on the concentration of gold nanoparticles for antibacterial activity. Adv. Nat. Sci. Nanosci. Nanotechnol. 12(3), 035003 (2021)
Ge, H., et al.: Preparation of organic–inorganic hybrid silica nanoparticles with contact antibacterial properties and their application in UV-curable coatings. Prog. Org. Coat. 106, 20–26 (2017)
Camporotondi, D., et al.: Antimicrobial properties of silica modified nanoparticles. Microb. Pathog. Strat. Combating Sci. Technol. Educ. 2, 283–290 (2013)
Mosselhy, D., et al.: Silica-gentamicin nanohybrids: synthesis and antimicrobial action. Materials 9(3), 170 (2016)
Acknowledgments
This study was financially supported from the National Science Fund of Bulgaria by Grant КP-06-H58/6-19.11.2021. The authors thank the Bulgarian Ministry of Education and Science for support: Scientific Infrastructure on Cell Technologies in Biomedicine (SICTB) D01-154/28/08/2018 and “National Center for Biomedical Photonics” D01-392/2020, part of Bulgarian National Roadmap for Scientific Infrastructures 2020–2027.
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Ivanova, I. et al. (2023). Stress Response of Gram-Positive and Gram-Negative Bacteria Induced by Metal and Non-metal Nanoparticles. In Search of Smart Antimicrobial Agents. In: Sotirov, S., Pencheva, T., Kacprzyk, J., Atanassov, K.T., Sotirova, E., Ribagin, S. (eds) Recent Contributions to Bioinformatics and Biomedical Sciences and Engineering. BioInfoMed 2022. Lecture Notes in Networks and Systems, vol 658. Springer, Cham. https://doi.org/10.1007/978-3-031-31069-0_15
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