Abstract
Protein is a vital component of a healthy diet and products derived from plant-based protein sources are being produced and consumed at an increasing rate. The presence of anti-nutritional factors, which limit bioavailability of plant protein, and a lack of sufficient essential amino acids to meet human nutritional requirements are concerning attributes for plant-based protein products and their nutritional quality. Of particular interest are the Protein Digestibility Corrected Amino Acid Score (PDCAAS), an indication of protein and amino acid bioavailability, and the Protein Efficiency Ratio (PER), a growth rate measurement based on quantity of protein consumed. Green methods for processing plant material have been garnering interest from product developers as well as consumers for their reduced environmental impact as well as enhancing the quantity and/or quality of the final protein produced. This chapter presents, where available, a discussion on the content and nutritional quality of plant proteins as determined by PER or PDCAAS following extraction by alkaline extraction and isoelectric precipitation, air classification, high-pressure processing, enzyme-assisted extraction, microwave-assisted extraction, reverse micelle protein extraction, and ultrasound-assisted extraction.
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References
Akyüz A, Ersus S (2021) Optimization of enzyme assisted extraction of protein from the sugar beet (Beta vulgaris L.) leaves for alternative plant protein concentrate production. Food Chem 335:127673
Barac M, Pesic M, Stanojevic S, Kostic A, Cabrilo S (2015) Techno-functional properties of pea (Pisum sativum) protein isolates-a review. Acta Periodica Technologica 46:1–17
Barba FJ, Poojary MM, Wang J, Olsen K, Orlien V (2017) Effect of high pressure processing and storage on the free amino acids in seedlings of Brussels sprouts. Innovative Food Sci Emerg Technol 41:188–192
Bayraktar E, Kocapiçak Ö, Mehmetoğlu Ü, Parlaktuna M, Mehmetoğlu TJCER, Design. (2008) Recovery of amino acids from reverse micellar solution by gas hydrate. Chem Eng Res Des 86(2):209–213
Benhammouche T, Melo A, Martins Z, Faria MA, Pinho SCM, Ferreira IMLPVO, Zaidi F (2021) Nutritional quality of protein concentrates from Moringa Oleifera leaves and in vitro digestibility. Food Chem 348:128858
Byanju B, Rahman MM, Hojilla-Evangelista MP, Lamsal BP (2020) Effect of high-power sonication pretreatment on extraction and some physicochemical properties of proteins from chickpea, kidney bean, and soybean. Int J Biol Macromol 145:712–721
Carbonaro M, Cappelloni M, Nicoli S, Lucarini M, Carnovale E (1997) Solubility−digestibility relationship of legume proteins. J Agric Food Chem 45(9):3387–3394
Charlebois S, Somogyi S, Music J (2018) Plant-based dieting and meat attachment: protein wars and the changing Canadian consumer (preliminary results), In edn. Dalhousie University, Halifax
Cheng F, Shu G, Chen L, Dai C, Wan H, Chen H, Dong X (2021) Ultrasound-microwave assisted extraction of proteins from Moringa oleifera leaves: comparative optimization study and LC-MS analysis of the protein concentrate. J Food Process Preserv 45(6)
Dhakad AK, Ikram M, Sharma S, Khan S, Pandey VV, Singh A (2019) Biological, nutritional, and therapeutic significance of<i>Moringa oleifera</i>lam. Phytother Res 33(11):2870–2903
Dong X, Zhao M, Shi J, Yang B, Li J, Luo D, Jiang G, Jiang Y (2011) Effects of combined high-pressure homogenization and enzymatic treatment on extraction yield, hydrolysis and function properties of peanut proteins. Innovative Food Sci Emerg Technol 12(4):478–483
Emkani M, Oliete B, Saurel R (2021) Pea protein extraction assisted by lactic fermentation: impact on protein profile and thermal properties. Foods 10(3):549
FAO (2013) Dietary protein quality evaluation in human nutrition: paper 92. Food and Agriculture Organization of the United Nations and The World Health Organization, Rome, Italy
FAO, & WHO (1991) Protein quality evaluation: report of the joint FAO/WHO expert consultation, FAO food and nutrition: paper 51. Food and Agriculture Organization of the United Nations, and the World Health Organization, Rome, Italy
Flórez N, Conde E, Domínguez H (2015) Microwave assisted water extraction of plant compounds. J Chem Technol Biotechnol 90(4):590–607
Gao Z, Shen P, Lan Y, Cui L, Ohm J-B, Chen B, Rao J (2020) Effect of alkaline extraction pH on structure properties, solubility, and beany flavor of yellow pea protein isolate. Food Res Int 131:109045
Ge Y, Sun A, Ni Y, Cai T (2000) Some nutritional and functional properties of defatted wheat germ protein. J Agric Food Chem 48(12):6215–6218
Gençdağ E, Görgüç A, Yılmaz FM (2021) Recent advances in the recovery techniques of plant-based proteins from agro-industrial by-products. Food Rev Intl 37(4):447–468
Gharibzahedi SMT, Smith B (2021) Effects of high hydrostatic pressure on the quality and functionality of protein isolates, concentrates, and hydrolysates derived from pulse legumes: a review. Trends Food Sci Technol 107:466–479
Golly MK, Ma H, Yuqing D, Dandan L, Quaisie J, Tuli JA, Mintah BK, Dzah CS, Agordoh PD (2020) Effect of multi-frequency countercurrent ultrasound treatment on extraction optimization, functional and structural properties of protein isolates from walnut (Juglans regiaL.) meal. J Food Biochem 44(6):e13210
Görgüç A, Özer P, Yılmaz FM (2020) Microwave-assisted enzymatic extraction of plant protein with antioxidant compounds from the food waste sesame bran: comparative optimization study and identification of metabolomics using LC/Q-TOF/MS. J Food Process Preserv 44(1)
Government of Canada (2020) Measuring the protein quality of foods. In: Ottawa, vol 2022. Health Canada, ON
Government of the United States of America (2018) Electronic code of federal regulations: Title 21 Food and drugs part 101 food labeling–101.13 Nutrient content claims–general principles. In, vol. 2018. Washington D.C
Government of the United States of America (2020) Electronic code of Federal Regulations: title 21 food and drugs part 101.9 nutrition labelling of food, vol 2020, Washington D.C
Gunawardena CK, Zijlstra RT, Beltranena E (2010) Characterization of the nutritional value of air-classified protein and starch fractions of field pea and zero-tannin faba bean in grower pigs. J Anim Sci 88(2):660–670
Health Canada. (2016). Health Canada Position–High Pressure Processing (HPP) Is No Longer a Novel Process. In, vol. 2022). Ottawa, Ontario Canada: Government of Canada
HealthCanada (1981) Determination of protein rating FO-1. In H. Canada (Ed.). http://www.hc-sc.gc.ca/fn-an/alt_formats/hpfbdgpsa/pdf/res-rech/fo-1-eng.pdf
International Food Information Council Foundation (2015) What's your health worth? Food and health survey 2015. In. Washington DC
International Food Information Council Foundation (2018) 2018 foods and health survey. In. Washington DC
International Food Information Council Foundation (2021) Plant and animal protein choices: consumer viewpoints and purchasing behaviors. In. Washington DC
Kentish S, Feng H (2014) Applications of power ultrasound in food Processing. Annu Rev Food Sci Technol 5(1):263–284
Khazaei H, Subedi M, Nickerson M, Martínez-Villaluenga C, Frias J, Vandenberg A (2019) Seed protein of lentils: current status, Progress, and food applications. Foods 8(9):391
Konieczny D, Stone AK, Nosworthy MG, House JD, Korber DR, Nickerson MT, Tanaka T (2020) Nutritional properties of pea protein-enriched flour treated with different proteases to varying degrees of hydrolysis. Cereal Chem 97(2):429–440
Kovács Á, Ganzler K, Simon-Sarkadi L (1998) Microwave-assisted extraction of free amino acids from foods. Zeitschrift für Lebensmitteluntersuchung und–Forschung A 207(1):26–30
Kumitch HM, Stone A, Nosworthy MG, Nickerson MT, House JD, Korber DR, Tanaka T (2020) Effect of fermentation time on the nutritional properties of pea protein-enriched flour fermented by aspergillus oryzae and aspergillus Niger. Cereal Chem 97(1):104–113
Latif S, Diosady LL, Anwar F (2008) Enzyme-assisted aqueous extraction of oil and protein from canola (Brassica napus L.) seeds. Eur J Lipid Sci Technol 110(10):887–892
Leser ME, Mrkoci K, Luisi PL (1993) Reverse micelles in protein separation: the use of silica for the back-transfer process. Biotechnol Bioeng 41(4):489–492
Marinangeli CPF, House JD (2017) Potential impact of the digestible indispensable amino acid score as a measure of protein quality on dietary regulations and health. Nutr Rev 75(8):658–667
Musielak G, Mierzwa D, Kroehnke J (2016) Food drying enhancement by ultrasound–a review. Trend Food Sci Technol 56:126–141
Nadar SS, Rao P, Rathod VK (2018) Enzyme assisted extraction of biomolecules as an approach to novel extraction technology: a review. Food Res Int 108:309–330
Náthia-Neves G, Alonso E (2021) Valorization of sunflower by-product using microwave-assisted extraction to obtain a rich protein flour: recovery of chlorogenic acid, phenolic content and antioxidant capacity. Food Bioprod Process 125:57–67
Nosworthy M, Tulbek M, House JD (2017) Does the concentration, isolation, or deflavoring of pea, lentil, and faba bean protein alter protein quality. Cereal Foods World 62(4):139–142
Nosworthy MG, House JD (2017) Factors influencing the quality of dietary proteins: implications for pulses. Cereal Chem 94(1):49–57
Ochoa-Rivas A, Nava-Valdez Y, Serna-Saldívar SO, Chuck-Hernández C (2017) Microwave and ultrasound to enhance protein extraction from Peanut flour under alkaline conditions: effects in yield and functional properties of protein isolates. Food Bioprocess Technol 10(3):543–555
Osborne TB, Mendel LB, Ferry EL (1919) A method of expressing numerically the growth-promoting value of proteins. J Biol Chem 37(2):223–229
Pelgrom PJM, Vissers AM, Boom RM, Schutyser MAI (2013) Dry fractionation for production of functional pea protein concentrates. Food Res Int 53(1):232–239
Perović MN, Knežević Jugović ZD, Antov MG (2020) Improved recovery of protein from soy grit by enzyme-assisted alkaline extraction. J Food Eng 276:109894
Phongthai S, Lim S-T, Rawdkuen S (2016) Optimization of microwave-assisted extraction of rice bran protein and its hydrolysates properties. J Cereal Sci 70:146–154
Poojary MM, Orlien V, Passamonti P, Olsen K (2017) Enzyme-assisted extraction enhancing the umami taste amino acids recovery from several cultivated mushrooms. Food Chem 234:236–244
Potin F, Lubbers S, Husson F, Saurel R (2019) Hemp (Cannabis sativa L.) protein extraction conditions affect extraction yield and protein quality. J Food Sci 84(12):3682–3690
Preece KE, Hooshyar N, Krijgsman AJ, Fryer PJ, Zuidam NJ (2017) Intensification of protein extraction from soybean processing materials using hydrodynamic cavitation. Innovative Food Sci Emerg Technol 41:47–55
Puri M, Sharma D, Barrow CJ (2012) Enzyme-assisted extraction of bioactives from plants. Trends Biotechnol 30(1):37–44
Rempel C, Li X, Geng X, Liu Q, Zhang Y (2020) Manufacture of defatted canola meal with enhanced nutritive composition by air classification on an industrial scale. J Sci Food Agric 100(2):764–774
Saldanha do Carmo C, Silventoinen P, Nordgård CT, Poudroux C, Dessev T, Zobel H, Holtekjølen AK, Draget KI, Holopainen-Mantila U, Knutsen SH, Sahlstrøm S (2020) Is dehulling of peas and faba beans necessary prior to dry fractionation for the production of protein- and starch-rich fractions? Impact on physical properties, chemical composition and techno-functional properties. J Food Eng 278:109937
Schutyser MAI, Pelgrom PJM, van der Goot AJ, Boom RM (2015) Dry fractionation for sustainable production of functional legume protein concentrates. Trends Food Sci Technol 45(2):327–335
Sęczyk Ł, Świeca M, Kapusta I, Gawlik-Dziki U (2019) Protein–phenolic interactions as a factor affecting the physicochemical properties of white bean proteins. Molecules 24(3):408
Su-Yeon K, Bo-Min K, Jung-Bong K, Poovan S, Heon-Woong K, So-Young K, Se-Na K, Young-Sook C, Han-Seok C, Ki-Moon P (2014) Effect of steaming, blanching, and high temperature/high pressure Processing on the amino acid contents of commonly consumed Korean vegetables and pulses. Preventive Nutrition and Food Science 19:220–226
Sun X-H, Zhu K-X, Zhou H-MJJ, o. C. S. (2008) Protein extraction from defatted wheat germ by reverse micelles: optimization of the forward extraction. J Cereal Sci 48(3):829–835
Sun X, Bandara N (2019) Applications of reverse micelles technique in food science: a comprehensive review. Trends Food Sci Technol 91:106–115
Sun X, Zhang W, Zhang L, Tian S, Chen F (2021) Effect of ultrasound-assisted extraction on the structure and emulsifying properties of peanut protein isolate. J Sci Food Agric 101(3):1150–1160
Tang D, Yin G, Li B, Li LJMFS, Technology. (2010) Ultrasound-assisted extraction of protein from brewer's spent grain. Modern Food Science and Technology 26(3):256–284
Tang J (2015) Unlocking potentials of microwaves for food safety and quality. J Food Sci 80(8):E1776–E1793
Tang S, Hettiarachchy NS, Shellhammer TH (2002) Protein extraction from heat-stabilized defatted rice bran. 1. Physical processing and enzyme treatments. J Agric Food Chem 50(25):7444–7448
Tome D (2013) Digestibility issues of vegetable versus animal proteins: protein and amino acid requirements–functional aspects. Food Nutr Bull 34(2):272–274
Trakselyte-Rupsiene K, Juodeikiene G, Cernauskas D, Bartkiene E, Klupsaite D, Zadeike D, Bendoraitiene J, Damasius J, Ignatavicius J, Sikorskaite-Gudziuniene S (2021) Integration of ultrasound into the development of plant-based protein hydrolysate and its bio-stimulatory effect for growth of wheat grain seedlings in vivo. Plan Theory 10(7):1319
Tyler R, Youngs C, Sosulski W (1981) Air classificaiton of legumes. I. Separation efficiency, yield, and compsoition of the starch and protein fractions. Cereal Chem 58:144–148
Vogelsang-O’Dwyer M, Petersen IL, Joehnke MS, Sørensen JC, Bez J, Detzel A, Busch M, Krueger M, O’Mahony JA, Arendt EK, Zannini E (2020) Comparison of Faba bean protein ingredients produced using dry fractionation and isoelectric precipitation: techno-functional nutritional and environmental performance. Foods 9(3):322
Wang F, Zhang Y, Xu L, Ma H (2020) An efficient ultrasound-assisted extraction method of pea protein and its effect on protein functional properties and biological activities. LWT 127:109348
Wang Z, Zhao X, Hu H, Wang M, Zhang X, Liu H (2021) Improved backward extraction of walnut protein using AOT reverse micelles with microwave and its characteristics. J Food Process Preserv 45(6)
Yang H, Gao J, Yang A, Chen H (2015) The ultrasound-treated soybean seeds improve edibility and nutritional quality of soybean sprouts. Food Res Int 77:704–710
Yang K, Xu T-R, Fu Y-H, Cai M, Xia Q-L, Guan R-F, Zou X-G, Sun P-L (2021) Effects of ultrasonic pre-treatment on physicochemical properties of proteins extracted from cold-pressed sesame cake. Food Res Int 139:109907
Zhao X, Zhang X, Liu H, Zhang G, Ao Q (2018) Functional, nutritional and flavor characteristic of soybean proteins obtained through reverse micelles. Food Hydrocoll 74:358–366
Zhao X, Zhu H, Chen JJF, Processing B (2015) Effects of sodium bis (2-ethylhexyl) sulfo succinate (AOT) reverse micelles on physicochemical properties of soy protein. Food Bioprod Process 94:500–506
Zhu K-X, Sun X-H, Chen Z-C, Peng W, Qian H-F, Zhou H-M (2010) Comparison of functional properties and secondary structures of defatted wheat germ proteins separated by reverse micelles and alkaline extraction and isoelectric precipitation. Food Chem 123(4):1163–1169
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Nosworthy, M.G., Marinangeli, C.P.F. (2023). Impact of Green Extraction Technologies on Plant Protein Content and Quality. In: Hernández-Álvarez, A.J., Mondor, M., Nosworthy, M.G. (eds) Green Protein Processing Technologies from Plants. Springer, Cham. https://doi.org/10.1007/978-3-031-16968-7_12
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