Abstract
There is a clinical need to measure local tissue oxygen saturation (StO2), oxy-, deoxy- and total haemoglobin concentration ([O2Hb], [HHb], [tHb]) in human tissue. The aim was to validate an oximeter called OxyVLS applying visible light spectroscopy (VLS) to determine these parameters without needing to assume a reduced scattering coefficient (μ′s). This problem is solved by appropriate calibrations. Compared to near-infrared spectroscopy (NIRS), OxyVLS determines the oxygenation in a much smaller more superficial volume of tissue, which is useful in many clinical cases. OxyVLS was validated in liquid phantoms with known StO2, [tHb], and μ′s and compared to frequency domain NIRS as a reliable reference. OxyVLS showed a high accuracy for all the mentioned parameters and was even able to measure μ′s. Thus, OxyVLS was successfully tested in vitro.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
McCarthy I (2006) The physiology of bone blood flow: a review. J Bone Joint Surg Am 88(Suppl 3):4–9
Prinzen FW, Bassingthwaighte JB (2000) Blood flow distributions by microsphere deposition methods. Cardiovasc Res 45:13–21
Holzle F, Rau A, Loeffelbein DJ et al (2010) Results of monitoring fasciocutaneous, myocutaneous, osteocutaneous and perforator flaps: 4-year experience with 166 cases. Int J Oral Maxillofac Surg 39:21–28
Disa JJ, Cordeiro PG, Hidalgo DA (1999) Efficacy of conventional monitoring techniques in free tissue transfer: an 11-year experience in 750 consecutive cases. Plast Reconstr Surg 104:97–101
Gardiner MD, Nanchahal J (2010) Strategies to ensure success of microvascular free tissue transfer. J Plast Reconstr Aesthet Surg 63:e665–e673
Keller A (2007) Noninvasive tissue oximetry for flap monitoring: an initial study. J Reconstr Microsurg 23:189–197
Liu H, Chance B, Hielscher AH et al (1995) Influence of blood vessels on the measurement of hemoglobin oxygenation as determined by time-resolved reflectance spectroscopy. Med Phys 22:1209–1217
Benaron DA, Parachikov IH, Cheong WF et al (2005) Design of a visible-light spectroscopy clinical tissue oximeter. J Biomed Opt 10:44005
Ho JK, Liakopoulos OJ, Crowley R et al (2009) In vivo detection of myocardial ischemia in pigs using visible light spectroscopy. Anesth Analg 108:1185–1192
Fox PM, Zeidler K, Carey J et al (2013) White light spectroscopy for free flap monitoring. Microsurgery 33:198–202
Friedland S, Benaron D, Coogan S et al (2007) Diagnosis of chronic mesenteric ischemia by visible light spectroscopy during endoscopy. Gastrointest Endosc 65:294–300
Harrison DK (2003) Optical measurements of tissue oxygen saturation in lower limb wound healing. Adv Exp Med Biol 540:265–269
Harrison DK (2012) Clinical applications of tissue oxygen saturation measurements. Adv Exp Med Biol 737:191–196
Kessler M, Frank K, Hoper J et al (1992) Reflection spectrometry. Adv Exp Med Biol 317:203–212
Wodick R, Lubbers DW (1973) Quantitative analysis of reflection spectra and other spectra of inhomogenous light paths conducted in multicomponent systems with the aid of interval analysis, I. Interval analysis applied to multicomponent systems with unknown, inhomogeneous light paths (author’s transl). Hoppe Seylers Z Physiol Chem 354:903–915
Wodick R, Lubbers DW (1973) Quantitative analysis of reflection spectra and other spectra with inhomogenous light paths conducted in multicomponent systems with the aid of interval analysis, II. Determination wavelength-dependent interferences and their constituent and unknown components in multicomponent systems by interval analysis (author’s transl). Hoppe Seylers Z Physiol Chem 354:916–922
Lubbers DW (1973) Spectrophotometric examination of tissue oxygenation. Adv Exp Med Biol 37A:45–54
Kleiser S, Nasseri N, Andresen B et al (2016) Comparison of tissue oximeters on a liquid phantom with adjustable optical properties. Biomed Opt Express 7:2973–2992
Arri SJ, Muehlemann T, Biallas M et al (2011) Precision of cerebral oxygenation and hemoglobin concentration measurements in neonates measured by near-infrared spectroscopy. J Biomed Opt 16:047005
Wolf U, Wolf M, Choi JH et al (2003) Mapping of hemodynamics on the human calf with near infrared spectroscopy and the influence of the adipose tissue thickness. Adv Exp Med Biol 510:225–230
Ijichi S, Kusaka T, Isobe K et al (2005) Developmental changes of optical properties in neonates determined by near-infrared time-resolved spectroscopy. Pediatr Res 58:568–573
Lubbers DW, Wodick R (1969) The examination of multicomponent systems in biological materials by means of a rapid scanning photometer. Appl Opt 8:1055–1062
Zijlstra WG, Buursma A, van Assendelft OW (2000) Visible and near infrared absorption spectra of human and animal haemoglobin determi- nation and application. VSP, Utrecht
Acknowledgments
MW declares that he is president of the board and co-founder of OxyPrem AG. SK is CTO and co-founder of OxyPrem AG.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Nature Switzerland AG
About this paper
Cite this paper
Nasseri, N., Kleiser, S., Wolf, U., Wolf, M. (2022). In Vitro Validation of a New Tissue Oximeter Using Visible Light. In: Scholkmann, F., LaManna, J., Wolf, U. (eds) Oxygen Transport to Tissue XLIII. Advances in Experimental Medicine and Biology, vol 1395. Springer, Cham. https://doi.org/10.1007/978-3-031-14190-4_36
Download citation
DOI: https://doi.org/10.1007/978-3-031-14190-4_36
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-14189-8
Online ISBN: 978-3-031-14190-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)