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Genome Editing advances in Soybean Improvement against Biotic and Abiotic Stresses

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Soybean Improvement

Abstract

New breeding technologies have enabled scientists to integrate, delete, or replace genes for developing new crop varieties with great ease as compared to conventional breeding approaches. Using site-specific genome editing tools, we are able to pinpoint exact locus and modify it according to desired trait. Among all the genome editing tools, clustered regularly interspersed short palindromic repeats and its associated Cas genes (CRISPR/Cas system) emerged as an ultimate revolutionary approach for editing genomes of almost all organisms (plants, animals, and humans). CRISPR system is playing a key role in editing the genomes of numerous crops for yield enhancement, augmented nutritional value, imparting disease resistance and addressing food security problems. During the last decade, cropping system has changed entirely due to modification of crop genomes by CRISPR technology for being employed to functional genomic studies, upgrading agronomic traits and combating abiotic and biotic stresses. This chapter focuses on engineering abiotic and biotic stress tolerance through CRISPR technology in soybean. Multiple Cas effectors such as, Cas9, CasX, Cas12, Cas13, and Cas14, are currently being used for precise genome editing of numerous crops. These Cas effectors are being used in genome modification of soybean to add, delete, or replace gene cassettes. Applications of CRISPR technology in soybean will result in the production of clean modified plants (without antibiotic resistance gene marker), to enhance yield potential under abiotic and biotic stresses.

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Authors and Affiliations

  1. Center of Advanced Studies in Agriculture and Food Security (CASAFS), University of Agriculture Faisalabad, Faisalabad, Pakistan

    Sabin Aslam, Ahmad Munir, Sultan Habibullah Khan & Aftab Ahmad

  2. Departmentof Plant Pathology, University of Agriculture, Faisalabad, Pakistan

    Hafiz Muhammad Usman Aslam

  3. Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan

    Aftab Ahmad

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  1. Sabin Aslam
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  2. Ahmad Munir
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  3. Hafiz Muhammad Usman Aslam
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  4. Sultan Habibullah Khan
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  5. Aftab Ahmad
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Correspondence to Aftab Ahmad .

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Editors and Affiliations

  1. MRCFC, Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India

    Shabir Hussain Wani

  2. MRCFC, Khudwani, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India

    Najeeb ul Rehman Sofi

  3. Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India

    Muhammad Ashraf Bhat

  4. Academic Specialist-Research, Soybean breeding and genetics, Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI, USA

    Feng Lin

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Aslam, S., Munir, A., Aslam, H.M.U., Khan, S.H., Ahmad, A. (2022). Genome Editing advances in Soybean Improvement against Biotic and Abiotic Stresses. In: Wani, S.H., Sofi, N.u.R., Bhat, M.A., Lin, F. (eds) Soybean Improvement. Springer, Cham. https://doi.org/10.1007/978-3-031-12232-3_13

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