Abstract
We present the development of a compact, easy-to-use device that implements a 3D microfluidic network with sensing sites, based on impedance spectroscopy techniques. The aim is to provide a Lab-on-Chip approach in applications where classification of microparticles is required, as well as morphological and volume studies. A complex colloidal mixture made of cell-resembling agarose microbeads suspended in aqueous medium was arranged to carry out microfluidic and impedance spectroscopy tests on the device. Preliminary impedance measurements show the effectiveness of the counting sub-system, displaying a good sensitivity in detecting the passing of a single bead over a sensing site. These results confirm the effectiveness of the system, and encourage further developments toward an implementation in actual biomedical scenarios. Possible applications can be found in 3D cell-cultures monitoring, blood analysis and diagnosis and blood-related diseases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gabriel, C., Gabriel, S., Corthout, Y.E.: The dielectric properties of biological tissues: I. Literature survey. Phys. Med. Biol. 41(11), 2231 (1996)
Xu, Y., Xie, X., Duan, Y., Wang, L., Cheng, Z., Cheng, J.: A review of impedance measurements of whole cells. Biosens. Bioelectron. 77, 824–836 (2016)
Veroli, A., et al.: High circular dichroism and robust performance in planar plasmonic metamaterial made of nano-comma-shaped resonators. JOSA B 36(11), 3079–3084 (2019)
Grosse, C., Tirado, M.C.: Low-frequency dielectric spectroscopy of colloidal suspensions. J. Non-Cryst. Solids 305(1–3), 386–392 (2002)
Song, H., et al.: A microfluidic impedance flow cytometer for identification of differentiation state of stem cells. Lab Chip 13(12), 2300–2310 (2013)
Diez-Silva, M., Dao, M., Han, J., Lim, C.T., Suresh, S.: Shape and biomechanical characteristics of human red blood cells in health and disease. MRS Bull. 35(5), 382–388 (2010)
Canali, C., Heiskanen, A., Muhammad, P., Pettersen, F.J., Hemmingsen, M., Emnéus, J.: Bioimpedance monitoring of 3D cell culturing - complementary electrode configurations for enhanced spatial sensitivity. Biosens. Bioelectron. 63, 72–79 (2015)
Buzzin, A., Asquini, R., Caputo, D., de Cesare, G.: On-glass integrated SU-8 waveguide and amorphous silicon photosensor for on-chip detection of biomolecules: feasibility study on hemoglobin sensing. Sensors 21(2), 415 (2021)
Costantini, F., et al.: Lab-on-chip system combining a microfluidic-ELISA with an array of amorphous silicon photosensors for the detection of celiac disease epitopes. Sens. Bio-Sens. Res. 6, 51–58 (2015)
Buzzin, A., Veroli, A., Alam, B., Maiolo, L., Marrani, M., Muzi, M.: Polymer nano-sieve for particle filtering in lab-on-chip devices. In: AIP Conference Proceedings, vol. 2145, no. 1, p. 020013. AIP Publishing LLC (2019)
Piedimonte, P., et al.: Silicon nanowires to detect electric signals from living cells. Mater. Res. Exp. 6(8), 084005 (2019)
Buzzin, A., Cupo, S., Giovine, E., de Cesare, G., Belfiore, N.P.: Compliant nano-pliers as a biomedical tool at the nanoscale: design simulation and fabrication. Micromachines 11(12), 1087 (2020)
Pal, N., Sharma, S., Gupta, S.: Sensitive and rapid detection of pathogenic bacteria in small volumes using impedance spectroscopy technique. Biosens. Bioelectron. 77, 270–276 (2016)
Iannascoli, L., et al.: Micro-incubator based on lab-on-glass technology for nanosatellite missions. In: Di Francia, G., et al. (eds.) AISEM 2019. LNEE, vol. 629, pp. 83–89. Springer, Cham (2020). https://doi.org/10.1007/978-3-030-37558-4_13
Stallcop, L.E., et al.: Razor-printed sticker microdevices for cell-based applications. Lab Chip 18(3), 451–462 (2018)
Pecora, A., et al.: Low-temperature polysilicon thin film transistors on polyimide substrates for electronics on plastic. Solid-State Electron. 52(3), 348–352 (2008)
Buzzin, A., et al.: Integrated 3D microfluidic device for impedance spectroscopy in lab-on-chip systems. In: International Workshop on Advances in Sensors and Interfaces, pp. 224–227. IEEE (2019)
Vurchio, F., et al.: Grasping and releasing agarose micro beads in water drops. Micromachines 10(7), 436 (2019)
Du, N., Chou, J., Kulla, E., Floriano, P.N., Christodoulides, N., McDevitt, J.T.: A disposable bio-nano-chip using agarose beads for high performance immunoassays. Biosens. Bioelectron. 28(1), 251–256 (2011)
Nweke, M.C., McCartney, R.G., Bracewell, D.G.: Mechanical characterisation of agarose-based chromatography resins for biopharmaceutical manufacture. J. Chromatogr. A 1530, 129–137 (2017)
Carminati, M.: Advances in high-resolution microscale impedance sensors. J. Sens. 2017, 1–15 (2017)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this paper
Cite this paper
Buzzin, A. et al. (2023). Integrated Hybrid Glass-Plastic Chip for Sorting and Counting of Microparticles in Biomedical Applications. In: Di Francia, G., Di Natale, C. (eds) Sensors and Microsystems. AISEM 2021. Lecture Notes in Electrical Engineering, vol 918. Springer, Cham. https://doi.org/10.1007/978-3-031-08136-1_7
Download citation
DOI: https://doi.org/10.1007/978-3-031-08136-1_7
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-08135-4
Online ISBN: 978-3-031-08136-1
eBook Packages: EngineeringEngineering (R0)