Abstract
The Chelex method of DNA extraction is suitable for extracting the DNA from a smaller amount of samples. This method is quick and straightforward and does not involve any harmful organic solvents. The basic protocol involves the extraction of DNA by adding samples to hot Chelex suspensions at pH 10–11. The alkalinity of resin suspension and exposure to heat result in disruption of the cell membrane. Heating also causes the double helix of DNA to denature. Resin beads bind to the cellular components, while DNA (and RNA) remains dissolved in the aqueous solution. Chelex resin also inhibits DNA degradation by chelating metal ions. This method is particularly beneficial for forensic applications, but it is not appropriate for large-scale DNA extraction. The resulting single-stranded DNA is less stable, therefore, not suitable for long-term storage and RFLP analysis.
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Gautam, A. (2022). DNA Isolation by Chelex Method. In: DNA and RNA Isolation Techniques for Non-Experts. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-030-94230-4_10
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DOI: https://doi.org/10.1007/978-3-030-94230-4_10
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