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COVID-19 Diagnostic Approaches: An Overview

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CMBEBIH 2021 (CMBEBIH 2021)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 84))

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Abstract

In the midst of the global COVID-19 pandemic, diagnostic approaches have played a critical role in tracking and fighting the virus. In the light of this, the state-of-the-art biosensing devices and procedures being repurposed for COVID-19 diagnosis are critically evaluated. Various COVID-19 diagnostic approaches are constantly being tested and optimized in order to ensure accurate diagnosis and reduce false results. This review spotlights diagnostic strategies such as: (1) Reverse transcription polymerase chain reaction (RT-PCR), (2) Enzyme-linked immunosorbent test (ELISA), (3) Chemiluminescent Immunoassay (CLIA), (4) Lateral flow immunoassay (LFIA), (5) Clustered Regularly Interspersed Palindromic Repeats (CRISPR-Cas13), (6) Clustered Regularly Interspersed Palindromic Repeats (CRISPR-Cas12a), (7) Loop-mediated Isothermal Amplification (LAMP-based colorimetric method), (8) Point-of-care tests (POC- RT-PCR), (9) Point-of-care tests (POC- Isothermal DNA amplification), (10) reactive oxygen species (ROS), (11) Chest imaging using computerized tomography (CT), (12) X-ray radiography (CXR), (13) Field-effect transistor (FET) in order to address different biomarkers correlated with COVID-19. The overview of these diagnostic approaches is given in terms of their strengths, limitations, time per analysis, costs, and availability. Finally, conclusions on diagnostic tests are highlighted.

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, International Burch University, Sarajevo, Bosnia and Herzegovina

    Zejneba Jassin, Amir Heric, Amar Mujkic & Ena Baralic

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  1. Zejneba Jassin
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  2. Amir Heric
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  3. Amar Mujkic
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  4. Ena Baralic
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Correspondence to Zejneba Jassin .

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Editors and Affiliations

  1. Verlab Ltd Sarajevo and Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

    Almir Badnjevic

  2. Verlab Ltd Sarajevo and International Burch University Sarajevo, Sarajevo, Bosnia and Herzegovina

    Lejla Gurbeta Pokvić

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© 2021 The Author(s), under exclusive license to Springer Nature Switzerland AG

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Jassin, Z., Heric, A., Mujkic, A., Baralic, E. (2021). COVID-19 Diagnostic Approaches: An Overview. In: Badnjevic, A., Gurbeta Pokvić, L. (eds) CMBEBIH 2021. CMBEBIH 2021. IFMBE Proceedings, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-030-73909-6_100

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  • DOI: https://doi.org/10.1007/978-3-030-73909-6_100

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-73908-9

  • Online ISBN: 978-3-030-73909-6

  • eBook Packages: EngineeringEngineering (R0)

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