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DNA Fingerprinting of Date Palm Pollen Sources and Their Relevance to Yield and Fruit Traits

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The Date Palm Genome, Vol. 1

Part of the book series: Compendium of Plant Genomes ((CPG))

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Abstract

The date palm (Phoenix dactylifera L.), 2n=36, is a dioecious long-lived monocotyledonous plant. It is a dioecious plant with male and female flowers borne on separate palms. In commercial plantations, artificial pollination is essential for economical yield, since the female inflorescences are hand pollinated with selected pollen from males. It is generally known that pollen from different sources affects the yield and fruit quality. The pollen sources influences the size and shape of the seeds which is known as the xenia effect, and directly affects fruit set, yield and fruit physical and chemical characteristics referred to as the metaxenia effect. Many experiments have shown that no constant trend is detected for the genetic relationship between the pollen sources and commercial female cultivars. There is a clear relationship between them due to the ecogenetics (gene-environment interactions) for both the selected pollen source and the commercial female cultivar. The phylogenetic relationships among date palms can be investigated through fingerprinting analysis using RAPD. The RAPD–PCR can detect the genetic variability and the relationships among date palms, and also estimate the expression of similarity using the Dice coefficient. PCR is an enzymatic reaction, so the outcome can be significantly influenced by the quality and concentration of template DNA, PCR component concentrations and PCR cycling conditions.

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References

  • Abbas MF, Abdul-Wahid AH, Abass KI (2014) Metaxenic effect in date palm (Phoenix dactylifera L.) fruit in relation to level of endogenous auxins. AAB Bioflux 6:40–44

    Google Scholar 

  • Al-Dous EK, George B, Al-Mahmoud ME et al (2011) De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera). Nat Biotech 29:521–527

    Article  CAS  Google Scholar 

  • Al-Mssallem IS, Hu S, Zhang X et al (2013) Genome sequence of the date palm (Phoenix dactylifera L.). Nat Com 4:2274

    Google Scholar 

  • Billotte N, Marseillac N, Brottier P et al (2004) Nuclear microsatellite markers for the date palm (Phoenix dactylifera L.): characterization and utility across the genus Phoenix and in other palm genera. Molec Ecol Not 4:256–258

    Article  CAS  Google Scholar 

  • Denney JO (1992) Xenia includes metaxenia. Hort Sci 27:722–728

    Google Scholar 

  • El-Hammady MM, Khalifa AS, El-Hammady AM (1977) The effect of date pollen on some physical and chemical characters of (Hayani variety). Res Bull 773 Fac Agric Ain Shams Univ, Cairo, Egypt

    Google Scholar 

  • El-Salhy AM, El-Bana AA, Abdel-Galil HA, Ahmed EF (2010) Effect of pollen grains suspensions spraying on yield and fruit quality of Saidy date palm cultivar. Acta Hort 882:329–336

    Article  Google Scholar 

  • El-Salhy AM, AbdAlla AY, Mostafa RAA (1997) Evaluation of some date palm male seedling in pollination of Zaghloul and Samani date palms under Assiut conditions. Assiut J Agric Sci 28:79–87

    Google Scholar 

  • Emad FSA (2015) GIS-Mapping aridity and rainfall water deficit of Egypt. J Agric Env Sci Dam Univ, Egypt 14:17–40

    Google Scholar 

  • Gros-Balthazard M (2013) Hybridization in the genus Phoenix: a review. Emir J Food Agric 25:831–842

    Article  Google Scholar 

  • Hamwieh A, Farah J, Moussally S et al (2010) Development of 1000 microsatellite markers across the date palm (Phoenix dactylifera L.) genome. Acta Hort 882:269–277

    Article  CAS  Google Scholar 

  • Hazzouri KM, Flowers JM, Visser HJ et al (2015) Whole genome re-sequencing of date palms yields insights into diversification of a fruit tree crop. Nat Com 6:8824

    Article  CAS  Google Scholar 

  • Leonardo H, Janet BM, Kevin MM (2018) Effect of high temperature on pollen morphology, plant growth and seed yield in quinoa (Chenopodium quinoa Willd.). J Agr Crop Sci 205:33–45

    Google Scholar 

  • Lynch M (1990) The similarity index and DNA fingerprinting. Mol Biol Evol 7:478–484

    CAS  PubMed  Google Scholar 

  • Merwad MA, Mostafa EAM, Ashour NE et al (2020) Evaluation, DNA fingerprinting and productivity of some superior seeded female Egyptian date palms. Inter J Latest Tech Eng Manag Appl Sci 9:68–75

    Google Scholar 

  • Merwad MA, Mostafa EAM, Saleh MMS et al (2015) Yield and fruit quality of Hayany date palm as affected by different pollen grain sources. Inter J ChemTech Res 8:544–549

    Google Scholar 

  • Moghaieb REA, Abdel-Hadi AA, Ahmed MRA, Hassan AGM (2010) Genetic diversity and sex determination in date palms (Phoenix dactylifera L.) based on DNA markers. Arab J Biotech 132:143–156

    Google Scholar 

  • Mohamed A Awad (2010) Pollination of date palm (Phoenix dactylifera L.) cv. Khenazy by pollen grain-water suspension spray. J Food Agric Envir 8 (3&4):313–317

    Google Scholar 

  • Mostafa EAM, Saleh MMS, Ashour NE et al (2016) The effect of metaxenia on fruit yield and the relation between some date palm pollinizers and two female cultivars using RAPD molecular markers. Res J Pharma Bio Chem Sci 7:971–984

    CAS  Google Scholar 

  • Mostafa EAM, Saleh MMS, Ashour NE et al (2018) Productivity, fruit properties and genetic diversity by molecular markers of four Egyptian female date palm cultivars and five different pollinizers. Biosc Res 15:166–175

    Google Scholar 

  • Mothew CA, Al-Rawi H, Al-Zubahidi A et al (1975) The effect of different type of pollination of individual trees with different pollens. Proc 3rd Inter Palm and Date Conf Nov 30-Dec 4 1975 Baghdad, Iraq, pp 1–17

    Google Scholar 

  • Mustafa EAM, Heiba SAA, Saleh MMS et al (2014) Effect of different pollinizer sources on yield, fruit characteristics and phylogenetic relationships with Amhat cv. date palm (Phoenix dactylifera L.) in Egypt using RAPD markers. Inter J Agric Res 9:331–343

    Article  Google Scholar 

  • Munir M, Alhajhoj MR, Sallam AAM et al (2020) Effects of indigenous and foreign pollinizers on the yield and fruit characteristics of date palm cultivar Khalas. Iraqi J Agric Sci 51:356–365

    Google Scholar 

  • Nixon R (1928) Immediate influence of pollen in determining the size and time of ripening of the fruit of the date palm. J Hered 19:241–255

    Article  Google Scholar 

  • Nixon RW (1935) Metaxenia in dates. Proc Amer Soc Hort Sci 32:221–226

    Google Scholar 

  • Olfati JA, Sheykhtaher Z, Qamgosar R et al (2010) Xenia and metaxenia on cucumber fruit and seed characteristics. Inter J Veget Sci 16:243–252

    Article  Google Scholar 

  • Osman AMA, Reuther W, Erickson LC (1974) Xenia and metaxenia studies in the date palm (Phoenix dactylifera L.). Date Grow Inst Rep 51:6–16

    Google Scholar 

  • Price AL, Patterson NJ, Plenge RM et al (2006) Principal components analysis corrects for stratification in genome-wide association studies. Nat Genet 38:904–909

    Article  CAS  PubMed  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genet 155:945–959

    Article  CAS  Google Scholar 

  • Robbertse PJ, Coetzer LA, Johannsmeier MF, Swart DJ (1996) Hass yield and fruit size as influenced by pollination and pollen donor: a joint progress report. South Afr Avocado Grow Assoc Yearb 19:63–67

    Google Scholar 

  • Sambrook J, Fritsch KF, Maniatis T (1989) Molecular cloning. 2nd ed. Cold Spring Harbor, New York

    Google Scholar 

  • Heiba Samy AA, Hoda Ali BM, Mostafa Esam AM et al (2015) The phylogenetic map between three pollinizers and their impact on fruit set, yield and fruit quality of Zaghloul and Samani date palms. Catarina 11:51–58

    Google Scholar 

  • Sarrwy SMA, Haiba AAA, Attia SAA et al (2014) Influence of different pollen grains sources on yield and fruit quality of Siwi date palm, and their phylogenetic relationships using RAPD markers. Mid East J Agric Res 3:330–337

    Google Scholar 

  • Sedgley M, Griffin AR (1989) Sexual reproduction in tree crops. Academic Press, London, UK

    Google Scholar 

  • Sedra H, Lashermes Philippe, Trouslot Pierre et al (1998) Identification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markers. Euphy 103:75–82

    Article  CAS  Google Scholar 

  • Shaheen MA, Bacha MA, Nasr TA (1989a) Effect of male type on fruit chemical properties in some date palm cultivars. Ann Agric Sci 34:265–281

    Google Scholar 

  • Shaheen MA, Bacha MA, Nasr TA (1989b) Effect of male type on fruit setting, yield and fruit physical properties in some date palm cultivars. Ann Agric 34:283–299

    Google Scholar 

  • Soliman SS, Kassem HA, Al-Obeed RS et al (2020) Influence of pollen source and its mineral content on fruit retention and quality of Kadary date palm cultivar (Phoenix dactylifera L.) J Chem Bio Phys Sci 10:437–446

    Google Scholar 

  • Sudhersan C, Jibil Manuel S, Al-Sabah L (2010) Xenic and metaxenic effect of Phoenix pusilla pollen on certain date palm cultivars. Acta Hort 882:297–302

    Article  Google Scholar 

  • Swingle WT (1928) Metaxenia in the date palm: possibly a hormone action by the embryo or endosperm. J Hered 19:257–268

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucl Acid Res 18:7213–7218

    Article  CAS  Google Scholar 

  • Welsh J, Chada K, Dalal SS et al (1992) Arbitrarily primed PCR fingerprinting of RNA. Nucl Acids Res 20:4965–4970

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Weingartner U, Kaeser O, Long M, Stamp P (2002) Combining cytoplasmic male sterility and xenia increases grain yield of maize hybrids. Crop Sci 42:1848–1856

    Article  Google Scholar 

  • White JW, Castillo JA (1990) Association between productivity, root growth and carbon isotope discrimination in Phaseolus vulgaris under water deficit. Austral J Plant Phys 17:189–198

    CAS  Google Scholar 

  • Williams JGK, Kublick AR, Livak KJ et al (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18:6531–6535

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zaid A, De Wet PF (2002) Climatic requirements of date palm In: Zaid A (ed) Date palm cultivation, Plant Prod Prot Paper 156 Rev. 1, FAO, Rome, pp 57–72

    Google Scholar 

  • Zhang H, Gao S, Lercher MJ et al (2012) EvolView, an online tool for visualizing, annotating and managing phylogenetic trees. Nucl Acids Res 40:W569–W572

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Correspondence to Mohamed M. S. Saleh .

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Saleh, M.M.S., Mostafa, E.A.M., Ashour, N.E., Heiba, S.A.A. (2021). DNA Fingerprinting of Date Palm Pollen Sources and Their Relevance to Yield and Fruit Traits. In: Al-Khayri, J.M., Jain, S.M., Johnson, D.V. (eds) The Date Palm Genome, Vol. 1. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-73746-7_6

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