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Enforced Expression of METCAM/MUC18 Decreases In Vitro Motility and Invasiveness and Tumorigenesis and In Vivo Tumorigenesis of Human Ovarian Cancer BG-1 Cells

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Ovarian Cancer: Molecular & Diagnostic Imaging and Treatment Strategies

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1330))

Abstract

Objectives: We tested if METCAM/MUC18 overexpression also plays a suppressor role in another human ovarian cancer cell line, BG-1, in addition to the SK-OV3 cell line.

Methods: Human ovarian cancer BG-1 cells were transfected with METCAM/MUC18 cDNA and G418-resistant clones expressing different levels of METCAM/MUC18 were isolated. These clones were used to test the effects of enforced expression of METCAM/MUC18 on in vitro motility, invasiveness, and anchorage-independent colony formation (in vitro tumorigenesis), and in vivo tumorigenesis after SC injection and after IP injection in female athymic nude mice.

Results: Overexpression of METCAM/MUC18 reduced in vitro motility and invasiveness of BG-1 cells and anchorage-independent colony formation (in vitro tumor formation). Higher expression of METCAM/MUC18 in BG-1 cells significantly reduced in vivo tumor proliferation of the BG-1 cells after IP injection (orthotopic route) of the clones in female nude mice, though it did not significantly affect in vivo tumor proliferation after SC injection (non-orthotopic route).

Conclusion: Similar to SK-OV3 cells, METCAM/MUC18 also plays a suppressor role in the progression of BG-1 cells in a xenograft mouse model.

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Abbreviations

CAM:

Cell adhesion molecule

huMETCAM/MUC18:

Human METCAM/MUC18

IHC:

Immunohistochemistry

IP :

Intraperitoneal

METCAM:

Metastasis-regulating cell adhesion molecule

SC :

Subcutaneous

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Acknowledgments

I thank Eugene Lee Son, Jonathan Geekai Chang, and Guofang Zeng for technical supports. This project was supported by the research funding of Emory University School of Medicine, Atlanta, GA, USA and funds of National Science Council, Taiwan.

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Authors and Affiliations

  1. Department of Microbiology & Immunology and Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, USA

    Guang-Jer Wu

  2. Department of Bioscience Technology and Center for Biomedical Technology, Chung Yuan Christian University, Taoyuan City, Taiwan

    Guang-Jer Wu

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Correspondence to Guang-Jer Wu .

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Editors and Affiliations

  1. Dept. Veterinary Pathobiology, University of Missouri-Columbia Dept. Veterinary Pathobiology, COLUMBIA, MO, USA

    Heide Schatten

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Wu, GJ. (2021). Enforced Expression of METCAM/MUC18 Decreases In Vitro Motility and Invasiveness and Tumorigenesis and In Vivo Tumorigenesis of Human Ovarian Cancer BG-1 Cells. In: Schatten, H. (eds) Ovarian Cancer: Molecular & Diagnostic Imaging and Treatment Strategies. Advances in Experimental Medicine and Biology, vol 1330. Springer, Cham. https://doi.org/10.1007/978-3-030-73359-9_8

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