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Targeted Breeding in Cotton Using CRISPR/Cas9 Genome Editing

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Cotton Precision Breeding

Abstract

Traditional breeding has made significant progress toward improving cotton adaptation in prevailing environments for increased yield and quality. The conventional breeding systems are handicapped owing to lengthy breeding process, random insertion of genes, and phenotype-based selections which are often misleading. Recently developed CRISPR/Cas9 genome editing technology opens a new era for acquiring precision cotton breeding. CRISPR/Cas9 is a naturally occurred genome editing tool adopted from prokaryotic adoptive immune defense system. Due to its simplicity and robustness, CRISPR/Cas9 has been quickly adopted to use in multiple fields, including gene function study and precise breeding. Since it was adopted in cotton for the first time in 2017, in which Li and colleagues edited an endogenous functional gene MYB-15 like in cotton, CRISPR/Cas9 has been quickly adopted by several cotton research laboratories and biotechnological industries worldwide. Currently, CRISPR/Cas9 has been widely used to study gene function and cotton improvement, including fiber development, resistance to abiotic and biotic stress, modification of cotton morphology, and secondary metabolism. In the next decade, CRISPR/Cas9-based precise breeding will significantly enhance cotton breeding and production.

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Acknowledgments

The CRISPR/Cas9-related research, including establishment of the CRISPR/Cas9 genome system in cotton, in B.Z. lab is supported in part by Cotton Incorporated and the National Science Foundation (award 1658709).

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Authors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

  2. Agricultural Biotechnology Division, National Institute for Biotechnology & Genetic Engineering (NIBGE) College, Faisalabad, Pakistan

    Mehboob-ur- Rahman

  3. Pakistan Institute for Engineering and Applied Science (PIEAS), Islamabad, Pakistan

    Mehboob-ur- Rahman

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  1. Baohong Zhang
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Correspondence to Baohong Zhang .

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Editors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology & Genetic Engineering (NIBGE) College, Faisalabad, Pakistan

    Mehboob-ur- Rahman

  2. Pakistan Agriculture Research Council, Islamabad, Pakistan

    Yusuf Zafar

  3. College of Agriculture and Biotechnology, Zhejiang University, Hanzhou, China

    Tianzhen Zhang

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Zhang, B., Rahman, Mu. (2021). Targeted Breeding in Cotton Using CRISPR/Cas9 Genome Editing. In: Rahman, Mu., Zafar, Y., Zhang, T. (eds) Cotton Precision Breeding. Springer, Cham. https://doi.org/10.1007/978-3-030-64504-5_14

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