Abstract
Nutritional and hormonal requirements of seedlings and shoots of Haloxylon recurvum and Haloxylon salicornicum were analyzed in vitro in present investigation. It occurs naturally in saline and sandy arid areas and belongs to family Amaranthaceae. Dry seeds were pretreated with 70% (v/v) alcohol for 10–20 sec and then sterilized with 0.1% (w/v) HgCl2 for 2–3 min followed by 4–5 washings with autoclaved distilled water and inoculated on hormone-free MS medium for germination. Cultures were maintained at 28 ± 2 °C under 35–40 μmol m−2 s−1 irradiance and 12hd−1 photoperiod by cool white fluorescent tubes. Seventy and 80% seeds of H. recurvum and H. salicornicum were germinated on hormone-free MS medium. Axillary buds of cotyledonary nodes of H. recurvum and H. salicornicum were activated on 6 μM BAP and 8 μM BAP respectively. Five and 3% (w/v) sucrose was found to be more appropriate for the growth and development of cotyledonary nodes of H. recurvum and H. salicornicum. In vitro cultured seedlings can be used as an efficient source of explants for establishment of cell culture and mass multiplication of shoots for industrial production of plant products and micropropagation of these plants.
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Acknowledgments
We thank the University Grants Commission (UGC), Government of India, for providing financial support through Major Research Project [F.No.39-411/2010 (SR)], “Scheme for providing additional assistance to Universities” [F.No.4-1/2011 (SU)], and CAS-I under Special Assistance Program (SAP) [No.F. 5-1/2013 (SAP-II)] to the Department of Botany, J N Vyas University, Jodhpur, India.
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Goswami, D., Dagla, H.R. (2021). In Vitro Culture of Haloxylon recurvum and Haloxylon salicornicum: Valuable Source of Food Additives and Pharmaceutical and Nutritional Components from Extreme Arid Zone. In: Malik, S. (eds) Exploring Plant Cells for the Production of Compounds of Interest. Springer, Cham. https://doi.org/10.1007/978-3-030-58271-5_12
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