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The Biology of mRNA: Structure and Function pp 247–284Cite as

Lessons from (pre-)mRNA Imaging

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1203))

Abstract

Cells are complex assemblies of molecules organized into organelles and membraneless compartments, each playing important roles in ensuring cellular homeostasis. The different steps of the gene expression pathway take place within these various cellular compartments, and studying gene regulation and RNA metabolism requires incorporating the spatial as well as temporal separation and progression of these processes. Microscopy has been a valuable tool to study RNA metabolism, as it allows the study of biomolecules in the context of intact individual cells, embryos or tissues, preserving cellular context often lost in experimental approaches that require the collection and lysis of cells in large numbers to obtain sufficient material for different types of assays. Indeed, from the first detection of RNAs and ribosomes in cells to today’s ability to study the behaviour of single RNA molecules in living cells, or the expression profile and localization of hundreds of mRNA simultaneously in cells, constant effort in developing tools for microscopy has extensively contributed to our understanding of gene regulation. In this chapter, we will describe the role various microscopy approaches have played in shaping our current understanding of mRNA metabolism and outline how continuous development of new approaches might help in finding answers to outstanding questions or help to look at old dogmas through a new lens.

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Acknowledgements

We thank members of the Zenklusen laboratory for discussion and comments on the manuscript. This work has been supported by Canadian Institutes of Health Research (Project Grant-366682), Le Fonds de recherche du Québec—Santé (Chercheur-boursier Junior 2) and Natural Sciences and Engineering Research Council of Canada for DZ.

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  1. Département de Biochimie et médecine moléculaire, Université de Montréal, Montréal, Québec, Canada

    Srivathsan Adivarahan & Daniel Zenklusen

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Correspondence to Daniel Zenklusen .

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  1. Systems biology (IRCM), Biochemistry and Molecular Medicine (UdeM), Experimental Medicine (McGill), Institut de recherches cliniques de Montréal (IRCM), Université de Montréal, McGill University, Montreal, QC, Canada

    Marlene Oeffinger

  2. Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC, Canada

    Daniel Zenklusen

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Adivarahan, S., Zenklusen, D. (2019). Lessons from (pre-)mRNA Imaging. In: Oeffinger, M., Zenklusen, D. (eds) The Biology of mRNA: Structure and Function . Advances in Experimental Medicine and Biology, vol 1203. Springer, Cham. https://doi.org/10.1007/978-3-030-31434-7_9

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