Abstract
Biodegradation is a cost-effective tool in the remediation of persistent organic pollutants but can take place only when the concentrations of toxic substances are decreased to levels suitable for microbial growth, i.e., after nZVI application. Assessment of the impact of nZVI on indigenous microbial communities is, therefore, an essential step in designing a successful remediation strategy. Here, we summarize the most common microbiological methods for monitoring the effect of nZVI on microorganisms such as the cultivation of bacteria, fluorescence in situ hybridization (FISH), phospholipid fatty acid (PLFA) analysis, real-time PCR, and sequencing.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
APHA (2005) Standard methods for the examination of water and wasterwater, 21st edn. American Public Health Association, Washington, DC
Baldrian P, Větrovský T, Cajthaml T, Dobiášová P, Petránková M, Šnajdr J, Eichlerová I (2013) Estimation of fungal biomass in forest litter and soil. Fungal Ecol 6(1):1–11. https://doi.org/10.1016/j.funeco.2012.10.002
Balvočiūtė M, Huson DH (2017) SILVA, RDP, Greengenes, NCBI and OTT—how do these taxonomies compare? BMC Genomics 18(2):114. https://doi.org/10.1186/s12864-017-3501-4
Barrero-Canosa J, Moraru C, Zeugner L, Fuchs BM, Amann R (2017) Direct-geneFISH: a simplified protocol for the simultaneous detection and quantification of genes and rRNA in microorganisms. Environ Microbiol 19(1):70–82. https://doi.org/10.1111/1462-2920.13432
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917. https://doi.org/10.1139/y59-099
Dolinová I, Czinnerová M, Dvořák L, Stejskal V, Ševců A, Černík M (2016) Dynamics of organohalide-respiring bacteria and their genes following in-situ chemical oxidation of chlorinated ethenes and biostimulation. Chemosphere 157:276–285. https://doi.org/10.1016/j.chemosphere.2016.05.030
Dolinová I, Štrojsová M, Černík M, Němeček J, Macháčková J, Ševců A (2017) Microbial degradation of chloroethenes: a review. Environ Sci Pollut Res 24(15):13262–13283. https://doi.org/10.1007/s11356-017-8867-y
Emerson D, Fleming EJ, McBeth JM (2010) Iron-oxidizing bacteria: an environmental and genomic perspective. Annu Rev Microbiol 64:561–583. https://doi.org/10.1146/annurev.micro.112408.134208
Kubota K (2013) CARD-FISH for environmental microorganisms: technical advancement and future applications. Microbes Environ 28(1):3–12. https://doi.org/10.1264/jsme2.ME12107
Němeček J, Lhotský O, Cajthaml T (2014) Nanoscale zero-valent iron application for in situ reduction of hexavalent chromium and its effects on indigenous microorganism populations. Sci Total Environ 485–486:739–747. https://doi.org/10.1016/j.scitotenv.2013.11.105
Němeček J, Dolinová I, Macháčková J, Špánek R, Ševců A, Lederer T, Černík M (2017) Stratification of chlorinated ethenes natural attenuation in an alluvial aquifer assessed by hydrochemical and biomolecular tools. Chemosphere 184:1157–1167. https://doi.org/10.1016/j.chemosphere.2017.06.100
Nguyen NHA, Špánek R, Kasalický V, Ribas D, Vlková D, Řeháková H, Kejzlar P, Ševců A (2018) Different effects of nano-scale and micro-scale zero-valent iron particles on planktonic microorganisms from natural reservoir water. Environ Sci Nano 5(5):1117–1129. https://doi.org/10.1039/C7EN01120B
Sampedro I, Giubilei M, Cajthaml T, Federici E, Federici F, Petruccioli M, D’annibale A (2009) Short-term impact of dry olive mill residue addition to soil on the resident microbiota. Bioresour Technol 100(23):6098–6106. https://doi.org/10.1016/j.biortech.2009.06.026
Stella T, Covino S, Burianová E, Filipová A, Křesinová Z, Voříšková J, Větrovský T, Baldrian P, Cajthaml T (2015) Chemical and microbiological characterization of an aged PCB-contaminated soil. Sci Total Environ 533:177–186. https://doi.org/10.1016/j.scitotenv.2015.06.019
Stella T, Covino S, Čvančarová M, Filipová A, Petruccioli M, D’Annibale A, Cajthaml T (2017) Bioremediation of long-term PCB-contaminated soil by white-rot fungi. J Hazard Mater 324:701–710. https://doi.org/10.1016/j.jhazmat.2016.11.044
Vogel M, Nijenhuis I, Lloyd J, Boothman C, Pöritz M, Mackenzie K (2018) Combined chemical and microbiological degradation of tetrachloroethene during the application of Carbo-Iron at a contaminated field site. Sci Total Environ 628–629:1027–1036. https://doi.org/10.1016/j.scitotenv.2018.01.310
Weber KA, Achenbach LA, Coates JD (2006) Microorganisms pumping iron: anaerobic microbial iron oxidation and reduction. Nat Rev Microbiol 4:752–764. https://doi.org/10.1038/nrmicro1490
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Ševců, A., Dolinová, I., Cajthaml, T., Steinová, J., Špánek, R. (2020). Tool V: Microbiological Methods for Monitoring nZVI Performance in Groundwater Conditions. In: Filip, J., Cajthaml, T., Najmanová, P., Černík, M., Zbořil, R. (eds) Advanced Nano-Bio Technologies for Water and Soil Treatment. Applied Environmental Science and Engineering for a Sustainable Future. Springer, Cham. https://doi.org/10.1007/978-3-030-29840-1_34
Download citation
DOI: https://doi.org/10.1007/978-3-030-29840-1_34
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-29839-5
Online ISBN: 978-3-030-29840-1
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)