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Determination of Antibody Structures

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Monoclonal Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1131))

Abstract

While antibodies share a conserved structural framework, their complementarity-determining region loops are highly variable in size and sequence. Even more variable are the potential ways these loops can be used to interact with antigen. Thus, X-ray crystal structures of antibody Fab fragments and Fab–antigen complexes are critical for a detailed understanding of the antibody–antigen recognition process. This chapter describes the basic procedures necessary for the crystallization and structure determination of antibody Fab fragments by X-ray crystallography.

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Acknowledgements

This is manuscript number 21975 from the Scripps Research Institute.

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Authors and Affiliations

  1. Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA

    Robyn L. Stanfield

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  1. Robyn L. Stanfield
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Editors and Affiliations

  1. Département de Biochimie, University of Geneva, Geneva, Switzerland

    Vincent Ossipow

  2. NovImmune SA, Plan-les-Ouates, Switzerland

    Nicolas Fischer

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Stanfield, R.L. (2014). Determination of Antibody Structures. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_23

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  • DOI: https://doi.org/10.1007/978-1-62703-992-5_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-991-8

  • Online ISBN: 978-1-62703-992-5

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