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Graphene–PAMAM Dendrimer–Gold Nanoparticle Composite for Electrochemical DNA Hybridization Detection

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Nucleic Acid Detection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1039))

Abstract

Graphene oxide is chemically functionalized using planar structured first generation polyamidoamine dendrimer (G1PAMAM) to form graphene core GG1PAMAM. The monolayer of GG1PAMAM is anchored on the 3-mercapto propionic acid monolayer pre-immobilized onto a gold transducer. The GG1PAMAM is decorated using gold nanoparticles for the covalent attachment of single-stranded DNA through simple gold-thiol chemistry. The single- and double-stranded DNAs are discriminated electrochemically in the presence of redox probe K3[Fe(CN)6]. Double-stranded-specific intercalator methylene blue is used to enhance the lower detection limit. The use of linear and planar G1PAMAM along with the graphene core has enhanced the detection limit 100 times higher than the G1PAMAM with the conventional ethylene core. This chapter presents the details of GG1PAMAM preparation and application to DNA sensing by electrochemical methods.

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Acknowledgments

Dr. V. Dharuman and K. Jayakumar acknowledge the Council of Scientific and Industrial Research, New Delhi, India for the financial support through project (CSIR No.03(1160)/10/EMR-II).

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Authors and Affiliations

  1. Molecular Electronics Lab, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, India

    Kumarasamy Jayakumar & Venkataraman Dharuman

  2. Department of Chemistry, Pondicherry University, Pondicherry, India

    Rajendiran Rajesh & Rangarajan Venkatesan

Authors
  1. Kumarasamy Jayakumar
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  2. Rajendiran Rajesh
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  3. Venkataraman Dharuman
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  4. Rangarajan Venkatesan
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Editors and Affiliations

  1. University of Central Florida Department of Chemistry, Orlando, Florida, USA

    Dmitry M. Kolpashchikov

  2. University of Central Florida Department of Chemistry, Orlando, Florida, USA

    Yulia V. Gerasimova

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Jayakumar, K., Rajesh, R., Dharuman, V., Venkatesan, R. (2013). Graphene–PAMAM Dendrimer–Gold Nanoparticle Composite for Electrochemical DNA Hybridization Detection. In: Kolpashchikov, D., Gerasimova, Y. (eds) Nucleic Acid Detection. Methods in Molecular Biology, vol 1039. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-535-4_17

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  • DOI: https://doi.org/10.1007/978-1-62703-535-4_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-534-7

  • Online ISBN: 978-1-62703-535-4

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