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Assessment of Inflammasome Activation in Primary Human Immune Cells

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The Inflammasome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1040))

Abstract

Inflammasomes are central regulators of inflammation, responsible for cleavage of the inactive pro-­inflammatory cytokines IL-1β and IL-18 into their biologically active counterparts. Several regulatory stages within the pathways responsible for the production of these cytokines have been identified. In this chapter, methods are described for assessing the degree of activity of these regulatory stages, which include mRNA transcription, caspase-1 activation, and secretion of the bioactive proteins.

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Acknowledgments

This work was supported by a Veni and a Vici grant of the Dutch Organization for Scientific Research (NWO) to Theo S. Plantinga and Mihai G. Netea, respectively.

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Authors and Affiliations

  1. Department of Medicine, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands

    Theo S. Plantinga, Leo A. B. Joosten & Mihai G. Netea

  2. Nijmegen Institute for Infection, Inflammation and Immunity (N4i), Nijmegen, The Netherlands

    Theo S. Plantinga, Leo A. B. Joosten & Mihai G. Netea

Authors
  1. Theo S. Plantinga
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  2. Leo A. B. Joosten
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  3. Mihai G. Netea
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Editor information

Editors and Affiliations

  1. , Institute of Innate Immunity, University of Bonn, Sigmund-Freud-Strasse 25, Bonn, 53105, Germany

    Christine M. De Nardo

  2. , Institute of Innate Immunity, University of Bonn, Sigmund-Freud-Str. 25, Bonn, 53105, Germany

    Eicke Latz

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© 2013 Springer Science+business media, New York

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Plantinga, T.S., Joosten, L.A.B., Netea, M.G. (2013). Assessment of Inflammasome Activation in Primary Human Immune Cells. In: De Nardo, C., Latz, E. (eds) The Inflammasome. Methods in Molecular Biology, vol 1040. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-523-1_4

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  • DOI: https://doi.org/10.1007/978-1-62703-523-1_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-522-4

  • Online ISBN: 978-1-62703-523-1

  • eBook Packages: Springer Protocols

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