Abstract
The ability to identify the donor stem cells following transplantation into injured hearts is critical. This is particularly important in evaluating stem cell survival and lineage differentiation into mature cardiovascular cells. Several approaches have been employed for tracking the donor stem cells, including fluorescent dyes and fluorescent protein gene transfer. Here, we will induce a protocol using lentivirus-mediated green fluorescent protein (GFP) to monitor the fate of donor stem cells following transplantation.
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Acknowledgement
This work was supported by the American Heart Association Beginning Grant-in-Aid 0765094Y (to Y.T.), NIH grant HL086555 (to Y.T.), and NIH grants HL076684 and HL62984 (to N.L.W.).
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Wang, Y., Zhang, L., Pan, Y., Chen, L., Weintraub, N., Tang, Y. (2013). Identification of Stem Cells After Transplantation. In: Kao, R. (eds) Cellular Cardiomyoplasty. Methods in Molecular Biology, vol 1036. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-511-8_8
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DOI: https://doi.org/10.1007/978-1-62703-511-8_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-510-1
Online ISBN: 978-1-62703-511-8
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