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Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis

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Capillary Electrophoresis of Biomolecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 984))

Abstract

Capillary electrophoresis (CE) is one among a number of highly sensitive chemical separation techniques used to characterize single or a small number of cells and to develop assays of enzymatic activity. Other commonly used techniques include mass spectrometry and electrochemistry; however, CE using laser-induced fluorescence detection (LIF) is the most sensitive of these techniques. In CE-LIF, fluorescently labeled proteins or lipids are normally separated based on their size to charge ratio in the interior of a small capillary filled with an electrolyte upon the application of an electric field. In this chapter, we describe the application of CE-LIF for the determination of the bioactivity of fluorescently lipids and sphingosine kinase activity.

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Acknowledgment

This research study was supported by the Kent State University research startup fund and the Farris Innovation award.

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Authors and Affiliations

  1. Department of Chemistry, Kent State University, Kent, OH, USA

    Philip M. Yangyuoru

  2. School of Science & Technology, Georgia Gwinnett College, Lawrenceville, GA, USA

    Latanya Hammonds-Odie

  3. School of Science and Technology, Georgia Gwinnett College, Lawrenceville, GA, USA

    Simon M. Mwongela

Authors
  1. Philip M. Yangyuoru
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  2. Latanya Hammonds-Odie
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  3. Simon M. Mwongela
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Corresponding author

Correspondence to Simon M. Mwongela .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213/D, Modena, 41125, Italy

    Nicola Volpi

  2. Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213/D, Modena, 41125, Italy

    Francesca Maccari

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Yangyuoru, P.M., Hammonds-Odie, L., Mwongela, S.M. (2013). Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_24

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  • DOI: https://doi.org/10.1007/978-1-62703-296-4_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-295-7

  • Online ISBN: 978-1-62703-296-4

  • eBook Packages: Springer Protocols

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