Abstract
The development of animal models of HPV infection has given investigators a new set of tools to expand basic knowledge of the early events of infection in vivo. The use of HPV pseudovirions, in which the viral genome has been replaced with a reporter pseudogenome, in combination with advanced imaging techniques has facilitated and simplified studies using these models. Herein we provide details for a murine model of cervicovaginal HPV infection in conjunction with several methods for imaging and quantitating the transduced genes, both ex vivo and in vivo.
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Acknowledgments
Research was supported by the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, NIH. Thanks to John Schiller and Jeff Roberts for their review of the text.
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Kines, R.C., Kobayashi, H., Choyke, P.L., Bernardo, M.L. (2013). Optical Imaging of HPV Infection in a Murine Model. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_7
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DOI: https://doi.org/10.1007/978-1-62703-227-8_7
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-226-1
Online ISBN: 978-1-62703-227-8
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