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Legionella pneumophila Typing

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Molecular Typing in Bacterial Infections

Part of the book series: Infectious Disease ((ID))

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Abstract

Molecular typing of Legionella has two principal applications: local epidemiology (e.g., outbreak investigation) and global epidemiology (e.g., population dynamics). In 1986, a European working group on Legionella infection (EWGLI) was created for the surveillance of Legionnaires’ disease (LD) in Europe. This group has been tasked to develop, evaluate, and improve several Legionella typing methods. Most Legionella molecular typing methods are based on comparison of DNA-banding patterns or sequence comparison. One of these methods, based on sequence comparison, the sequence-based typing (SBT) appears to be a powerful tool for global epidemiology (rapidity, ease of results exchange, high inter-laboratory reproducibility). Using it for international surveillance allows observation that particular genotypes (ST1, ST23, ST40, ST47, etc.) could be spread worldwide and responsible for a large part of culture-proven LD cases. For local epidemiological purpose, several methods have demonstrated their efficiency (SBT, PFGE, MLVA), in some cases combining these methods could enhance their discriminatory index (e.g., SBT + MAb subgrouping + PFGE).

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Authors and Affiliations

  1. Laboratoire pathogénie bactérienne et immunité innée, Université Lyon 1, Faculté de médecine Lyon est, INSERM U851, Centre national de référence des légionelles, Hospices civils de Lyon, 7 rue Guillaume Paradin, 69372, Lyon, France

    Christophe Ginevra

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  1. Christophe Ginevra
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Correspondence to Christophe Ginevra .

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Editors and Affiliations

  1. Instituto Nacional de Controle de, Qualidade em Saúde, Fundação Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Rio de Janeiro, 21045-900, Rio de Janeiro, Brazil

    Ivano de Filippis

  2. Microbiology, BioReliance Corporation, Broschart Road 14920, Rockville, 20850, Maryland, USA

    Marian L. McKee

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Ginevra, C. (2013). Legionella pneumophila Typing. In: de Filippis, I., McKee, M. (eds) Molecular Typing in Bacterial Infections. Infectious Disease. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-185-1_15

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  • DOI: https://doi.org/10.1007/978-1-62703-185-1_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-184-4

  • Online ISBN: 978-1-62703-185-1

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