Abstract
Nanoparticle Tracking Analysis (NTA) is an image analysis-based technique that deduces the particle size from the changing position of scattering diffusing particles in a sequence of images. It is shown that a basic understanding of the underlying physical principles largely helps to prevent artifacts. In fact, an inappropriate selection of both software parameters, such as maximum particle jump or minimum track length, as well as sample preparation aspects (such as degree of dilution) may give rise to inaccurate or even erroneous results.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Buzea C, Pacheco I, Robbie K (2007) Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2(4):MR17–MR71
Gill S, Lobenberg R, Ku T et al (2007) Nanoparticles: characteristics, mechanisms of action, and toxicity in pulmonary drug delivery—a review. J Biomed Nanotechnol 3:107–119
Grassian VH (2008) When size really matters: size-dependent properties and surface chemistry of metal and metal oxide nanoparticles in gas and liquid phase environments. J Phys Chem C 112:18303–18313
Finder C, Wohlgemuth M, Mayer C (2004) Analysis of particle size distribution by particle tracking. Part Part Syst Charact 21:372–378
Xu CS, Cang H, Montiel D, Yang H (2007) Rapid and quantitative sizing of nanoparticles using three-dimensional single-particle tracking. J Phys Chem C 111:32–35
Malloy A, Carr B (2006) Nanoparticle tracking analysis—the halo (TM) system. Part Part Syst Charact 23:197–204
Ryu JH, Bang SY, Yoon JW et al (2007) Pulsed laser induced synthesis of scheelite-type colloidal nanoparticles in liquid and the size distribution by nanoparticle tracking analysis. Appl Surf Sci 253:8408–8414
Kusaka Y, Adachi Y (2007) Determination of hydrodynamic diameter of small flocs by means of direct movie analysis of Brownian motion. Colloids Surf A Physicochem Eng Asp 306:166–170
Hiemenz PC (1986) Principles of colloid and surface chemistry, 2nd edn. Marcel Dekker, New York
Saxton MJ (1997) Single-particle tracking: the distribution of diffusion coefficients. Biophys J 72:1744–1753
Hertz P (1909) Uber den gegenseitigen durchschnittlichen Abstand von Punkten, die mit bekannter mittlerer Dichte im Raume angeordnet sind. Math Ann 67:387–398
Torquato S, Lu B, Rubinstein J (1990) Nearest-neighbour distribution functions in many-body systems. Phys Rev A 41:2059–2075
Saveyn H, De Baets B, Thas O et al (2010) Accurate particle size distribution determination by nanoparticle tracking analysis based on 2-D Brownian dynamics simulation. J Colloid Interface Sci 352:593–600
Acknowledgments
Patrick Hole (Nanosight Ltd, UK) is kindly acknowledged for the stimulating discussions related to the NTA theoretical background.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Van der Meeren, P., Kasinos, M., Saveyn, H. (2012). Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_42
Download citation
DOI: https://doi.org/10.1007/978-1-61779-953-2_42
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-952-5
Online ISBN: 978-1-61779-953-2
eBook Packages: Springer Protocols