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  • © 2012

Protein Electrophoresis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB)

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Table of contents (58 protocols)

  1. Front Matter

    Pages i-xiv
  2. Introduction to Protein Electrophoresis

    • Pothur R. Srinivas
    Pages 23-28
  3. Protein Solubilization: Attend to the Choice of Lysis Buffer

    • Mandy Peach, Noelle Marsh, Daniel J. MacPhee
    Pages 37-47
  4. Native Polyacrylamide Gels

    • Claudia Arndt, Stefanie Koristka, Holger Bartsch, Michael Bachmann
    Pages 49-53
  5. Cationic Electrophoresis

    • Engelbert Buxbaum
    Pages 55-63
  6. Tricine-SDS-PAGE

    • Syed R. Haider, Helen J. Reid, Barry L. Sharp
    Pages 81-91
  7. Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis

    • Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen
    Pages 93-109
  8. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis

    • Henghang Tsai, Hon-chiu Eastwood Leung
    Pages 119-134
  9. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples

    • Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, Peter Hoffmann
    Pages 135-146
  10. Microchip Electrophoresis, with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”

    • Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, Jean-Louis Viovy
    Pages 173-184
  11. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

    • Mustafa Çelebier, Clara Ibáñez, Carolina Simó, Alejandro Cifuentes
    Pages 185-195
  12. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE

    • Yassel Ramos, Vladimir Besada, Lila Castellanos-Serra
    Pages 197-204
  13. Tris–Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass

    • Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, Jose Luis Rosa
    Pages 205-213

About this book

Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.

Editors and Affiliations

  • Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, Oklahoma City, USA

    Biji T. Kurien

  • Oklahoma Medical Research Foundation, Oklahoma City, USA

    R. Hal Scofield

Bibliographic Information

  • Book Title: Protein Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Biji T. Kurien, R. Hal Scofield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-821-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-820-7Published: 16 May 2012

  • Softcover ISBN: 978-1-4939-6241-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-821-4Published: 15 May 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 648

  • Number of Illustrations: 120 b/w illustrations, 31 illustrations in colour

  • Topics: Protein Science, Proteomics, Electrophoresis

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access