Skip to main content
SpringerLink
Log in

Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells

  • Protocol
  • First Online:
Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

Islet transplantation is a widely accepted and practiced cell replacement therapy for treatment of diabetes. However, scarcity of suitable cadaveric pancreas donors is a major limitation that restricts the availability of this therapy to millions of diabetic individuals worldwide. Research in the field has therefore focused on search for an alternate cell source. Various stem/progenitor cells have been considered to be suitable for replacement therapy in diabetes since they have the potential to proliferate and differentiate. Over last few years, we have specifically focused our attention on understanding the potential of progenitor cells that are derived from in vitro expansion of human islets. Since epigenetic marks that define an “active” insulin promoter region in beta cells are inherited during in vitro expansion, we believe that human islet-derived progenitor cells (hIPCs) represent a lineage-committed population of islet precursor cells. Here, we describe details of the method for isolation, expansion, and characterization of human islet-derived progenitor cells (hIPCs).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Phadnis SM, Ghaskadbi SM, Hardikar AA, Bhonde RR (2009) Mesenchymal stem cells derived from bone marrow of diabetic patients portrait unique markers influenced by the diabetic microenvironment. Rev Diabet Stud 6(4):260–270

    Article  PubMed  Google Scholar 

  2. Phadnis SM, Joglekar MV, Dalvi MP et al (2011) Human bone marrow-derived mesenchymal cells differentiate and mature into endocrine pancreatic lineage in vivo. Cyto-therapy 13(3):279–293

    Article  PubMed  Google Scholar 

  3. Gershengorn MC, Hardikar AA, Wei C, Geras-Raaka E, Marcus-Samuels B, Raaka BM (2004) Epithelial-to-mesenchymal transition generates proliferative human islet precursor cells. Science 306(5705):2261–2264

    Article  PubMed  CAS  Google Scholar 

  4. Joglekar MV, Hardikar AA (2010) Epithelial-to-mesenchymal transition in pancreatic islet beta cells. Cell Cycle 9(20):4077–4079

    Article  PubMed  CAS  Google Scholar 

  5. Joglekar MV, Joglekar VM, Hardikar AA (2009) Expression of islet-specific microRNAs during human pancreatic development. Gene Expr Patterns 9(2):109–113

    Article  PubMed  CAS  Google Scholar 

  6. Joglekar MV, Joglekar VM, Joglekar SV, Hardikar AA (2009) Human fetal pancreatic insulin-producing cells proliferate in vitro. J Endocrinol 201(1):27–36

    Article  PubMed  CAS  Google Scholar 

  7. Hardikar AA, Marcus-Samuels B, Geras-Raaka E, Raaka BM, Gershengorn MC (2003) Human pancreatic precursor cells secrete FGF2 to stimulate clustering into hormone-expressing islet-like cell aggregates. Proc Natl Acad Sci U S A 100(12):7117–7122

    Article  PubMed  CAS  Google Scholar 

  8. Bonner-Weir S, Taneja M, Weir GC et al (2000) In vitro cultivation of human islets from expanded ductal tissue. Proc Natl Acad Sci U S A 97(14):7999–8004

    Article  PubMed  CAS  Google Scholar 

  9. Parekh VS, Joglekar MV, Hardikar AA (2009) Differentiation of human umbilical cord blood-derived mononuclear cells to endocrine pancreatic lineage. Differentiation 78(4):232–240

    Article  PubMed  CAS  Google Scholar 

  10. Sahu S, Tosh D, Hardikar AA (2009) New sources of beta-cells for treating diabetes. J Endocrinol 202(1):13–16

    Article  PubMed  CAS  Google Scholar 

  11. Sahu S, Joglekar MV, Dumbre R, Phadnis SM, Tosh D, Hardikar AA (2009) Islet-like cell clusters occur naturally in human gall bladder and are retained in diabetic conditions. J Cell Mol Med 13(5):999–1000

    Article  PubMed  Google Scholar 

  12. Maehr R, Chen S, Snitow M et al (2009) Generation of pluripotent stem cells from patients with type 1 diabetes. Proc Natl Acad Sci U S A 106(37):15768–15773

    Article  PubMed  CAS  Google Scholar 

  13. Alipio Z, Liao W, Roemer EJ et al (2010) Reversal of hyperglycemia in diabetic mouse models using induced-pluripotent stem (iPS)-derived pancreatic beta-like cells. Proc Natl Acad Sci U S A 107(30):13426–13431

    Article  PubMed  CAS  Google Scholar 

  14. Park IH, Arora N, Huo H et al (2008) Disease-specific induced pluripotent stem cells. Cell 134(5):877–886

    Article  PubMed  CAS  Google Scholar 

  15. Fujikawa T, Oh SH, Pi L, Hatch HM, Shupe T, Petersen BE (2005) Teratoma formation leads to failure of treatment for type I diabetes using embryonic stem cell-derived insulin-producing cells. Am J Pathol 166(6):1781–1791

    Article  PubMed  CAS  Google Scholar 

  16. Russ HA, Bar Y, Ravassard P, Efrat S (2008) In vitro proliferation of cells derived from adult human beta-cells revealed by cell-lineage tracing. Diabetes 57(6):1575–1583

    Article  PubMed  CAS  Google Scholar 

  17. Russ HA, Ravassard P, Kerr-Conte J, Pattou F, Efrat S (2009) Epithelial-mesenchymal transition in cells expanded in vitro from lineage-traced adult human pancreatic beta cells. PLoS One 4(7):e6417

    Article  PubMed  Google Scholar 

  18. Joglekar MV, Hardikar AA (2010) Epithelial-to-mesenchymal transition in pancreatic islet beta cells. Cell Cycle 9(20):4077–4079

    Article  PubMed  CAS  Google Scholar 

  19. Joglekar MV, Patil D, Joglekar VM et al (2009) The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells. Islets 1(2):137–147

    Article  PubMed  Google Scholar 

  20. Teta M, Rankin MM, Long SY, Stein GM, Kushner JA (2007) Growth and regeneration of adult beta cells does not involve specialized progenitors. Dev Cell 12(5):817–826

    Article  PubMed  CAS  Google Scholar 

  21. Hauser C, Schuettengruber B, Bartl S, Lagger G, Seiser C (2002) Activation of the mouse histone deacetylase 1 gene by cooperative histone phosphorylation and acetylation. Mol Cell Biol 22(22):7820–7830

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Council of Scientific and Industrial Research (CSIR) and National Center for Cell Science, Department of Biotechnology (DBT), Government of India, for funding/supporting the work presented in this chapter. The data presented in this chapter was generated through support from the Department of Biotechnology, Government of India microRNA consortium project grant (BT/PR7975/MED/14/1211/2006) to AAH. MVJ is supported by the Juvenile Diabetes Research Foundation postdoctoral award (JDRF 3-2011-352). AAH is an Australian Future Fellow and is supported by Australian Research Council (FT110100254).

Author information

Authors and Affiliations

  1. Stem cells and Diabetes section, National Center for Cell Science, Pune, MH, India

    Mugdha V. Joglekar & Anandwardhan A. Hardikar

  2. Immunology and Diabetes unit, St. Vincent’s Institute of Medical Research, Fitzroy, VIC, Australia

    Mugdha V. Joglekar

  3. Islet Biology and Diabetes Group, NHMRC Clinical Trials Centre, The University of Sydney, Camperdown, Australia

    Anandwardhan A. Hardikar

Authors
  1. Mugdha V. Joglekar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anandwardhan A. Hardikar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anandwardhan A. Hardikar .

Editor information

Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Joglekar, M.V., Hardikar, A.A. (2012). Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_21

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-815-3_21

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation