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Laser Capture Microdissection of Candida albicans from Host Tissue

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Host-Fungus Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 845))

Abstract

Laser microdissection is a technique in which specific populations of cells are acquired from sections of complex tissue under direct microscopic visualization. The technique can be used to selectively harvest or ablate host and/or fungal cells from a variety of biological specimens, including human, animal, or plant tissue sections. When coupled with downstream applications such as proteomic and molecular analyses, laser microdissection can address a variety of important biological questions specifically related to the in vivo host-fungus interaction. In this chapter, we describe how laser microdissection enables researchers to selectively isolate Candida albicans cells from host-infected tissue. Detailed protocols are provided for tissue handling and processing, slide preparation, and laser capture microdissection (LCM). Using these methods, we highlight the use of LCM to examine infection-related C. albicans gene expression.

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Acknowledgments

The authors thank Dr. Edward Balish, Dr. Bernhard Hube, Dr. Bart Frank, and Dr. Debra Hazen-Martin for informative discussions. We also thank the Gnotobiotic Animal Research Core at the Medical University of South Carolina. This work was supported by NIH grants R43DE017033, R21AI078098, and R21AI076721.

Author information

Authors and Affiliations

  1. Department of Craniofacial Biology, Medical University of South Carolina, Charleston, SC, USA

    Caroline Westwater

  2. Guild Associates Inc., Charleston, SC, USA

    David A. Schofield

Authors
  1. Caroline Westwater
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  2. David A. Schofield
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Corresponding author

Correspondence to Caroline Westwater .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Alexandra C. Brand

  2. Institute of Medical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Donna M. MacCallum

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Westwater, C., Schofield, D.A. (2012). Laser Capture Microdissection of Candida albicans from Host Tissue. In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_27

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  • DOI: https://doi.org/10.1007/978-1-61779-539-8_27

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-538-1

  • Online ISBN: 978-1-61779-539-8

  • eBook Packages: Springer Protocols

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