Abstract
As mucus is highly hydrated, special care has to be taken to preserve this in histological preparations during immunostaining. Here, we describe how to fix tissues in such a way that the mucus is preserved in paraffin-embedded tissue. We also describe how the major macromolecular components in the mucus, the mucins, are immunostained and how bacteria can be localized in preparations with preserved mucus by fluorescent in situ hybridization.
Key words
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Johansson, M. E. V., Holmen Larsson, J. M., and Hansson, G. C. (2010) Proc. Natl. Acad. Sci. USA on-line.
Atuma, C., Strugula, V., Allen, A., and Holm, L. (2001) Am. J. Physiol. Gastrointest. Liver Physiol. 280, G922–G929.
Johansson, M. E., Gustafsson, J. K., Sjoberg, K. E., Petersson, J., Holm, L., Sjovall, H., and Hansson, G. C. (2010) PLoS. ONE. 5.
Johansson, M. E. V., Phillipson, M., Petersson, J., Holm, L., Velcich, A., and Hansson, G. C. (2008) Proc. Natl. Acad. Sci. USA 105, 15064–15069.
Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., and Stahl, D. A. (1990) Appl. Environ. Microbiol. 56, 1919–1925.
Acknowledgments
This work was supported by the Swedish Research Council (No. 7461, 21027, and 342-2004-4434), The Swedish Cancer Foundation, The Knut and Alice Wallenberg Foundation (KAW2007.0118), IngaBritt and Arne Lundberg Foundation, Sahlgren’s University Hospital (LUA-ALF), EU-FP7 IBDase (no. 200931), Wilhelm and Martina Lundgren’s Foundation, Torsten och Ragnar Söderbergs Stiftelser, Swedish CF Foundation, and The Swedish Foundation for Strategic Research—The Mucosal Immunobiology and Vaccine Center (MIVAC) and the Mucus-Bacteria-Colitis Center (MBC) of the Innate Immunity Program (2010–2014).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Johansson, M.E.V., Hansson, G.C. (2012). Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH. In: McGuckin, M., Thornton, D. (eds) Mucins. Methods in Molecular Biology, vol 842. Humana Press. https://doi.org/10.1007/978-1-61779-513-8_13
Download citation
DOI: https://doi.org/10.1007/978-1-61779-513-8_13
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-512-1
Online ISBN: 978-1-61779-513-8
eBook Packages: Springer Protocols