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Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis

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Liver Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 826))

Abstract

A severely malfunctioning liver, due to acute liver injury or chronic liver disease, can lead to hepatic failure. The ultimate treatment for hepatic failure is liver transplantation; however, the availability of donors is a critical issue. Therefore, regenerative therapy is an anticipated novel approach for restoring liver function. Mesenchymal stem cells are pluripotent somatic cells that can differentiate into several cell types, including hepatocytes. Moreover, they are obtainable from easily accessible autologous adipose tissue, making them ideal for regenerative therapy. This chapter describes experimental methods for isolating mesenchymal stem cells from murine adipose tissues and expanding them, and also describes murine chronic liver disease, steatohepatitis, for the study of experimental regenerative treatments of chronic liver disease.

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Authors and Affiliations

  1. Institute of Industrial Science, University of Tokyo, Tokyo, Japan

    Yoshio Sakai

  2. Center for Liver Diseases, Kanazawa University Hospital, Kanazawa, Japan

    Shuichi Kaneko

  3. Department of Gastroenterology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan

    Shuichi Kaneko

Authors
  1. Yoshio Sakai
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  2. Shuichi Kaneko
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Corresponding author

Correspondence to Shuichi Kaneko .

Editor information

Editors and Affiliations

  1. Research Inst., National Cancer Center, Tsukiji 5-1-1, Tokyo, 104-0045, Japan

    Takahiro Ochiya

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Sakai, Y., Kaneko, S. (2012). Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis. In: Ochiya, T. (eds) Liver Stem Cells. Methods in Molecular Biology, vol 826. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-468-1_17

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  • DOI: https://doi.org/10.1007/978-1-61779-468-1_17

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-467-4

  • Online ISBN: 978-1-61779-468-1

  • eBook Packages: Springer Protocols

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